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An Sda page gel was run to find the molecular weight of protein X- In.docx

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An Sda page gel was run to find the molecular weight of protein X- In.docx

An Sda page gel was run to find the molecular weight of protein X. In a typical gel which has 10lanes

just to let you know the botton picture is number and and first picture is number 2. please answer in alphabetical order

Name: d) What is the molecular weight volue of protein bonds in lane 3 of the gel 4 points) Based on the volue of molecular weights you obtained in part d, what can you say about the oligomeric (monomer/dimer/trimer/tetromer) state of protein X on the gel? e) (2 points) ) Based on the molecular weight for the monomer in part d, how many picomoles of protein was loaded (2 points) in lane 37 g) When protein X was crystallized it was proven that it exists a homopentamer. What will be the molecular weight of this pentameric protein? (2 points) What is the optimal (minimum) amount (ug) of protein needed to run protein X on a gel? Explain your reasoning for picking this amount? h) (2 points)
Solution
(a) Rf = distance migrated/gel length
(b) Rf value in 3 lane will be
Rf = 4.45cm / 6.38 cm
Rf =0.6
(c) ?g of lane 2= 15000
?g of lane 3 = 75000
?g of lane 4 = 5000
?g of lane 5 - 2500
.

An Sda page gel was run to find the molecular weight of protein X. In a typical gel which has 10lanes

just to let you know the botton picture is number and and first picture is number 2. please answer in alphabetical order

Name: d) What is the molecular weight volue of protein bonds in lane 3 of the gel 4 points) Based on the volue of molecular weights you obtained in part d, what can you say about the oligomeric (monomer/dimer/trimer/tetromer) state of protein X on the gel? e) (2 points) ) Based on the molecular weight for the monomer in part d, how many picomoles of protein was loaded (2 points) in lane 37 g) When protein X was crystallized it was proven that it exists a homopentamer. What will be the molecular weight of this pentameric protein? (2 points) What is the optimal (minimum) amount (ug) of protein needed to run protein X on a gel? Explain your reasoning for picking this amount? h) (2 points)
Solution
(a) Rf = distance migrated/gel length
(b) Rf value in 3 lane will be
Rf = 4.45cm / 6.38 cm
Rf =0.6
(c) ?g of lane 2= 15000
?g of lane 3 = 75000
?g of lane 4 = 5000
?g of lane 5 - 2500
.

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An Sda page gel was run to find the molecular weight of protein X- In.docx

1. 1. An Sda page gel was run to find the molecular weight of protein X. In a typical gel which has 10lanes just to let you know the botton picture is number and and first picture is number 2. please answer in alphabetical order Name: d) What is the molecular weight volue of protein bonds in lane 3 of the gel 4 points) Based on the volue of molecular weights you obtained in part d, what can you say about the oligomeric (monomer/dimer/trimer/tetromer) state of protein X on the gel? e) (2 points) ) Based on the molecular weight for the monomer in part d, how many picomoles of protein was loaded (2 points) in lane 37 g) When protein X was crystallized it was proven that it exists a homopentamer. What will be the molecular weight of this pentameric protein? (2 points) What is the optimal (minimum) amount (ug) of protein needed to run protein X on a gel? Explain your reasoning for picking this amount? h) (2 points) Solution (a) Rf = distance migrated/gel length (b) Rf value in 3 lane will be Rf = 4.45cm / 6.38 cm Rf =0.6 (c) ?g of lane 2= 15000 ?g of lane 3 = 75000 ?g of lane 4 = 5000 ?g of lane 5 - 2500