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Transferring research
findings into industry
     application

  Dr. Craig F. Morris, Director
          USDA-ARS
Western Wheat Quality Laboratory
 Pullman, Washington, U. S. of A.
Need for new raw materials, consumer foods,
   and solutions to processing problems
                Consumer

            Food Manufacturer

                  Miller

               Merchandizer

                 Farmer

                 Breeding

                 Research
Research, new traits and new varieties –
 new and more consistent raw materials
                Research

                Breeding

                 Farmer

              Merchandizer

                 Miller

           Food Manufacturer

               Consumer
Wheat is used in a nearly limitless
      variety of foods and is the
leading cereal for human consumption
Humans have „interacted‟ with wheat
       for thousands of years

   We have influenced wheat evolution
      and wheat has influenced us

    We have learned to exploit genetic
variation in wheat to make different foods

We now manipulate wheat to expand the
    ways in which we can utilize it
Milling and end-use
quality of wheat results
from the composition
of the kernel, and
especially the
endosperm, but the
bran is becoming more
important
Grain composition and physical
    features also result from an
interaction between the „genotype‟
  and the environment in which
          the plant grows
We should think of milling and
end-use quality as „phenotypes‟ that
  are genetically based, and that
      we can understand and
  manipulate in the same way as,
       say, disease resistance

Breeding for quality should be no
more mysterious than breeding for
         rust resistance
There is no absolute
     definition of wheat or flour quality

     End-use quality of wheat is defined
by milling performance, processing efficiency,
             and specific end-uses
   for example, bread vs. noodles vs. cake
We often want to measure wheat „quality‟
       -- to quantify the physical
        and chemical differences

        Measurement of quality
       always involves some error
 and depends on the instrument/method
               being used
Our aim is to predict
     commercial processing and
        end-product quality
        through small-scale
          laboratory tests

These tests may target one constituent/
trait or aim to sum a number of traits
Major end-use quality traits of wheat

* kernel texture - „hardness‟
  also kernel color, size, shape
* milling performance - particle size,
   starch damage, flour yield, etc.
* gluten strength & amount
* water absorption
* arabinoxylans - „pentosans‟
* starch pasting / amylose content
* pigments / color
* enzymes - PPO, α-amylase, etc.
Genetic „cascade‟ for quality

  example: puroindoline gene expression

puroindoline haplotype (Pina-D1 / Pinb-D1)
→ puroindoline a and b expression
→ kernel texture differences
→ flour starch damage/granularity
→ dough water relations
→ processing and baking performance
→ end-product quality
Two examples of transferring research
     findings into industry application

       (and applying an understanding
  of industry needs into research activities)

       Both involve challenging some
pre-conceptions of kernel texture and quality

* An end-use quality model for soft wheat
* Development of soft durum
Why soft wheat?
             Why hard wheat?

  When hexaploid wheat was first formed,
   it was soft, and soft wheats reached
      Europe, China, Japan, Canada,
        the U.S., and Australia first

            So why hard wheat?

 The answer has almost nothing to do with
quality, and yet everything to do with quality
Historical context:

    When people migrate, they usually take
        their possessions with them,
            including their crops

Raising something is better than raising nothing

     Disease resistance, and adaptation to
     other biotic and abiotic stresses is key
                to growing food
Neolithic wheat movement (soft)
Early colonial wheat movement (soft)
Late 1800‟s wheat movement (hard)
Hard wheats ascended because:

   1) they were disease resistant and well
        adapted to heat and drought
        (not because they were hard)

2) they also happened to have stronger gluten
           and made superior bread

 3) invention of the purifier, “high milling”
  and then the steel roller mill facilitated
        their technological adoption
The current model for soft wheat quality:

              Soft kernel
        High break flour yield
     Low pentosans (arabinoxylans)

These tend to produce fine-textured flours
       with low starch damage and
           low water absorption
   (cf. large AACCI cookie diameters)

        What else would we like?
Gluten strength?
     Sometimes weak, but not always

              Good cake quality?
difficult to define/predict –must bake a cake

               White bran?
    Generally preferred, but preferred
           in hard wheats, too

               Low PPO?
      Good for soft and hard wheats
Partial waxy/reduced amylose?
       Depends on the use --
For udon soft bite noodles, absolutely

               Pigments?
        Consumer preference
         (cf. pasta/semolina)
What else?
   How about price?!

Aug. 2012 local delivery,
 Pullman, Washington:

Soft white $327/metric ton
  Hard red winter $351
  Hard red spring $379
What are you paying for?
            Kernel hardness?

No. First, wheat that will process on your mill,
    but more importantly, gluten strength

          Local Washington prices:
Hard red spring wheat –target of 14% protein
        For each 1% above, +$3/ton
        For each 1% below, -$10/ton
Bakery flour $660/mt

Vital wheat gluten $1,000/mt
So the baker should be thinking:

“What is the cheapest way to buy gluten strength?”

     “How much strength do I really need?”
Gluten strength has little if anything to do
           with kernel texture
Evolving model for soft wheat quality

Are soft wheats going to get stronger?
                 Yes.

Do we need some weak-gluten wheats?
               Yes.

      The question will be,
“How will we segregate the market?”
Second example of transferring
          research findings into
           industry application

       Development of soft durum

Acknowledgements: Leonard Joppa, Marco
     Simeone, Domenico Lafiandra,
       Jeff Casper, Jodi Engleson
Durum is characterized by its very hard kernel texture
Kernel texture influences damaged starch
               and flour granularity




Soft hexaploid   Hard hexaploid     Durum semolina
Conventional thinking:

“Durum wheat is used to make pasta because of
 its hard nature, which produces a firm cooked
 product. Semolina is coarsely ground durum
with a texture somewhat like sugar. It is the best
               product for pasta.”

 - U.S. National Association of Wheat Growers
Current situation:


Most durum is milled into semolina
  Semolina has 4-5% damaged starch
  Semolina particle size is about 3-4x that of
  regular flour
  Semolina is expensive
Issues with standard durum:

* Larger particles require longer hydration times
* Reducing particle size creates higher starch
   damage
* Higher starch damage requires increased water
* High water absorption increases production
   costs, mixing and drying times, and energy
   requirements
* Non-uniform particle size results in uneven
   hydration and pasta defects
So why is durum hard?
       By design?
            No.

It was an accident of nature

Can we make durum soft?
      Absolutely!
Soft durum milling properties
             are similar to soft wheat

* Commercial milling trial has been conducted
* Soft durum mills like soft wheat
* SDF has the particle size distribution of soft wheat
  flour with similar low starch damage
* Ash content equivalent to soft wheat flour
* Power required to mill is significantly reduced
* No need for complex milling flow required to
  produce semolina
Durum flour       Soft Durum Flour




500 μm                 500 µm




100 µm                 100 µm
Pilot Scale Pasta Tests
* Wheat SKCS = 24.0
* 17% wheat protein; 15.5% flour protein; ash 0.57%
* Median flour particle size 57 microns
* The SDF had starch and gluten that was similar to
  semolina based on testing in the Farinograph,
  Alveograph, and Rapid Visco Analyzer
* Optimum paste water absorption 28-29%
  (14% m.b.) vs. 32% for semolina
This shows defects of normal commercial
semolina at the very low 28-29% water absorption …
note the white strands with uneven water absorption.
This defect was not observed in the SDF
Checking observed in semolina pasta but not SDF
Soft durum makes high quality pasta

* Pasta can be produced with 10-15% less water

* Drying rates are similar; lower absorption translates
  into shorter drying times and energy savings

* Pasta trials: SDF is as good as or better than semolina
* Lower cooking loss
* Firmer cooked product over longer cook times
  (cooking tolerance)
* Reduced propensity for checking (packaging)
Soft durum can make novel bakery products

Soft durum flour can be used to make bakery
products with novel yellow color, richer flavor
and chewier texture than traditional baking flours

Gluten strength of SDF is the same as durum
wheat in general (no D-genome)
HRS bread (L), Soft durum pan bread (R)
Key benefits of Soft Durum:

To millers:
   - Do not need dedicated durum assets;
         higher throughput, higher yields(?)
To pasta manufacturers:
   - Reduced energy consumption
To bakers:
   - New and appealing artisan breads,
         pizza products , etc.
Thank you!

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Transferring research findings into industry application

  • 1. Transferring research findings into industry application Dr. Craig F. Morris, Director USDA-ARS Western Wheat Quality Laboratory Pullman, Washington, U. S. of A.
  • 2. Need for new raw materials, consumer foods, and solutions to processing problems Consumer Food Manufacturer Miller Merchandizer Farmer Breeding Research
  • 3. Research, new traits and new varieties – new and more consistent raw materials Research Breeding Farmer Merchandizer Miller Food Manufacturer Consumer
  • 4. Wheat is used in a nearly limitless variety of foods and is the leading cereal for human consumption
  • 5.
  • 6.
  • 7.
  • 8. Humans have „interacted‟ with wheat for thousands of years We have influenced wheat evolution and wheat has influenced us We have learned to exploit genetic variation in wheat to make different foods We now manipulate wheat to expand the ways in which we can utilize it
  • 9. Milling and end-use quality of wheat results from the composition of the kernel, and especially the endosperm, but the bran is becoming more important
  • 10. Grain composition and physical features also result from an interaction between the „genotype‟ and the environment in which the plant grows
  • 11. We should think of milling and end-use quality as „phenotypes‟ that are genetically based, and that we can understand and manipulate in the same way as, say, disease resistance Breeding for quality should be no more mysterious than breeding for rust resistance
  • 12. There is no absolute definition of wheat or flour quality End-use quality of wheat is defined by milling performance, processing efficiency, and specific end-uses for example, bread vs. noodles vs. cake
  • 13. We often want to measure wheat „quality‟ -- to quantify the physical and chemical differences Measurement of quality always involves some error and depends on the instrument/method being used
  • 14. Our aim is to predict commercial processing and end-product quality through small-scale laboratory tests These tests may target one constituent/ trait or aim to sum a number of traits
  • 15. Major end-use quality traits of wheat * kernel texture - „hardness‟ also kernel color, size, shape * milling performance - particle size, starch damage, flour yield, etc. * gluten strength & amount * water absorption * arabinoxylans - „pentosans‟ * starch pasting / amylose content * pigments / color * enzymes - PPO, α-amylase, etc.
  • 16. Genetic „cascade‟ for quality example: puroindoline gene expression puroindoline haplotype (Pina-D1 / Pinb-D1) → puroindoline a and b expression → kernel texture differences → flour starch damage/granularity → dough water relations → processing and baking performance → end-product quality
  • 17. Two examples of transferring research findings into industry application (and applying an understanding of industry needs into research activities) Both involve challenging some pre-conceptions of kernel texture and quality * An end-use quality model for soft wheat * Development of soft durum
  • 18. Why soft wheat? Why hard wheat? When hexaploid wheat was first formed, it was soft, and soft wheats reached Europe, China, Japan, Canada, the U.S., and Australia first So why hard wheat? The answer has almost nothing to do with quality, and yet everything to do with quality
  • 19. Historical context: When people migrate, they usually take their possessions with them, including their crops Raising something is better than raising nothing Disease resistance, and adaptation to other biotic and abiotic stresses is key to growing food
  • 21. Early colonial wheat movement (soft)
  • 22. Late 1800‟s wheat movement (hard)
  • 23. Hard wheats ascended because: 1) they were disease resistant and well adapted to heat and drought (not because they were hard) 2) they also happened to have stronger gluten and made superior bread 3) invention of the purifier, “high milling” and then the steel roller mill facilitated their technological adoption
  • 24. The current model for soft wheat quality: Soft kernel High break flour yield Low pentosans (arabinoxylans) These tend to produce fine-textured flours with low starch damage and low water absorption (cf. large AACCI cookie diameters) What else would we like?
  • 25. Gluten strength? Sometimes weak, but not always Good cake quality? difficult to define/predict –must bake a cake White bran? Generally preferred, but preferred in hard wheats, too Low PPO? Good for soft and hard wheats
  • 26. Partial waxy/reduced amylose? Depends on the use -- For udon soft bite noodles, absolutely Pigments? Consumer preference (cf. pasta/semolina)
  • 27. What else? How about price?! Aug. 2012 local delivery, Pullman, Washington: Soft white $327/metric ton Hard red winter $351 Hard red spring $379
  • 28. What are you paying for? Kernel hardness? No. First, wheat that will process on your mill, but more importantly, gluten strength Local Washington prices: Hard red spring wheat –target of 14% protein For each 1% above, +$3/ton For each 1% below, -$10/ton
  • 29. Bakery flour $660/mt Vital wheat gluten $1,000/mt
  • 30. So the baker should be thinking: “What is the cheapest way to buy gluten strength?” “How much strength do I really need?”
  • 31. Gluten strength has little if anything to do with kernel texture
  • 32. Evolving model for soft wheat quality Are soft wheats going to get stronger? Yes. Do we need some weak-gluten wheats? Yes. The question will be, “How will we segregate the market?”
  • 33.
  • 34. Second example of transferring research findings into industry application Development of soft durum Acknowledgements: Leonard Joppa, Marco Simeone, Domenico Lafiandra, Jeff Casper, Jodi Engleson
  • 35. Durum is characterized by its very hard kernel texture
  • 36. Kernel texture influences damaged starch and flour granularity Soft hexaploid Hard hexaploid Durum semolina
  • 37. Conventional thinking: “Durum wheat is used to make pasta because of its hard nature, which produces a firm cooked product. Semolina is coarsely ground durum with a texture somewhat like sugar. It is the best product for pasta.” - U.S. National Association of Wheat Growers
  • 38. Current situation: Most durum is milled into semolina Semolina has 4-5% damaged starch Semolina particle size is about 3-4x that of regular flour Semolina is expensive
  • 39. Issues with standard durum: * Larger particles require longer hydration times * Reducing particle size creates higher starch damage * Higher starch damage requires increased water * High water absorption increases production costs, mixing and drying times, and energy requirements * Non-uniform particle size results in uneven hydration and pasta defects
  • 40. So why is durum hard? By design? No. It was an accident of nature Can we make durum soft? Absolutely!
  • 41. Soft durum milling properties are similar to soft wheat * Commercial milling trial has been conducted * Soft durum mills like soft wheat * SDF has the particle size distribution of soft wheat flour with similar low starch damage * Ash content equivalent to soft wheat flour * Power required to mill is significantly reduced * No need for complex milling flow required to produce semolina
  • 42. Durum flour Soft Durum Flour 500 μm 500 µm 100 µm 100 µm
  • 43. Pilot Scale Pasta Tests * Wheat SKCS = 24.0 * 17% wheat protein; 15.5% flour protein; ash 0.57% * Median flour particle size 57 microns * The SDF had starch and gluten that was similar to semolina based on testing in the Farinograph, Alveograph, and Rapid Visco Analyzer * Optimum paste water absorption 28-29% (14% m.b.) vs. 32% for semolina
  • 44. This shows defects of normal commercial semolina at the very low 28-29% water absorption … note the white strands with uneven water absorption. This defect was not observed in the SDF
  • 45. Checking observed in semolina pasta but not SDF
  • 46. Soft durum makes high quality pasta * Pasta can be produced with 10-15% less water * Drying rates are similar; lower absorption translates into shorter drying times and energy savings * Pasta trials: SDF is as good as or better than semolina * Lower cooking loss * Firmer cooked product over longer cook times (cooking tolerance) * Reduced propensity for checking (packaging)
  • 47. Soft durum can make novel bakery products Soft durum flour can be used to make bakery products with novel yellow color, richer flavor and chewier texture than traditional baking flours Gluten strength of SDF is the same as durum wheat in general (no D-genome)
  • 48.
  • 49. HRS bread (L), Soft durum pan bread (R)
  • 50. Key benefits of Soft Durum: To millers: - Do not need dedicated durum assets; higher throughput, higher yields(?) To pasta manufacturers: - Reduced energy consumption To bakers: - New and appealing artisan breads, pizza products , etc.