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Everything you ever  needed to know  about everything
Animal Cells
Plant Cells
Carbohydrates Starch, Glycogen & Cellulose Sucrose, Lactose Glucose, Fructose
Types of Lipids Triglycerides Phospholipids
The nucleotide monomer PHOSPHATE SUGAR Nucleic acids contain C, H & O in addition to N & P
Proteins 3 groups: Amino group, Carboxyl group & R-group When joined together they form peptide bonds Aside from the C, H, O & N in the base molecule, the R group may also contain S & P
Protein structure Primary structure The linear sequence of amino acids Secondary structure The type of peptide bond determines how sections of the protein fold – spiral helix / pleated sheet / random coils Shape reinforced by additional H bonds Tertiary structure Eventual 3D shape formed by folding Shape reinforced by additional H bonds Quanternary structure When a protein is formed by the interaction of 2 or more polypeptide chains
Summary
Elements of a plasma membrane carbohydrate  chains glycolipid glycoprotein phospholipid bilayer protein channel cholesterol
How does it regulate molecular transport? Passive Transport Diffusion Osmosis Facilitated diffusion (channel, receptor & carrier mediated) Active Transport Primary (uses chemical energy) Secondary (uses electrochemical gradient)
Endocytosis
Exocytosis
[object Object]
Hypotonic: (hypo - lower) surrounding solution has a lower concentration than the cells.Water will diffuse through the membrane into the cells ,[object Object],(hyper - higher) surrounding solution has a higher concentration than the cells
Mitochondrion Energy supplying organelle Produces chemical energy in the form of adenosine triphosphate (ATP) through the process of cellular respiration Outer & inner membrane The inner membrane contains many folds to provide a larger surface area for energy production ATP produced by reactions on inner membrane Only in eukaryotes
Chloroplasts
Exergonic and Endergonic Reactions
Enzyme Structure Enzymes have an active site and a regulatory region The active site (formed by folds in the protein) is where substrate binds to the enzyme The regulatory region is where cofactors coenzymes or enzyme inhibitors can alter the function of an enzyme Substrate Products Active site Enzyme inhibitor Regulatory region
Factors Affecting Enzyme Activity pH Temperature Pressure Enzyme Concentration Substrate concentration Cofactors / coenzymes Inhibition
A simplified version of photosynthesis
Light  Dependent Reaction
Light  Independent Reaction
Light-dependent reaction Occurs in the grana Light energy is used to split water in to two H+ ions and O2 gas The O2 is released as waste With the power of the two free electrons One H+ ion fuses ADP to Pi to form ATP One H+ ion fuses to NADP to form NADPH
Light-independent stage Occurs in Stroma Does not need light, but NADPH and ATP from previous stage Needs CO2 and H+ions  Sugar molecules are synthesised from CO2 		CO2 = oxidised state (low E compound) 		C(H2O)n = reduced state (high E compound) NADPH (carrier H+) is the reducing agent ATP is the energy source
C3 PLANTS C4 PLANTS
Putting Photosynthesis together 2 x PGAL = fructose fructose = glucose fructose + glucose = sucrose glucose x ∞= starch
Cellular Respiration
Glycolysis Occurs in cytosol – uses enzymes and vitamins as coenzymes 1 glucose (6C) converted to 2 pyruvate (3C) Forms 2 ATP & 2 NADH
Krebs Cycle Occurs in mitochondria Pyruvate initially broken down in to CO2 and Acetyl-coA Joins with 4C molecule to form 6 C molecule CO2 to form 5 C molecule, then again to form 4 C molecule Further oxidation takes place to reform original 4C Throughout cycle, constant oxidation is fusing hydrogen to carrier molecules NAD -> NADH and FAD -> FADH2
Electron Transport Occurs in inner membrane of mitochondria Produces 2-3 ATP per loaded receptor Electrons passed from one cytochrome to next until accepted by O2- to form water Return of released protons through ATP synthase carrier provides energy to produce ATP from ADP & Pi (phosphorylation)
Anaerobic respiration (in humans) Occurs in muscles where oxygen supply exceeds demand The only stage that can occur is glycolysis So 1 glucose produces 2 ATP 2 NADH convert pyruvate to lactate (lactic acid) Lactate build up causes pH to fall and pain & muscle fatigue When activity returns to normal and oxygen becomes available, lactate converted back to pyruvate to enter the Krebs Cycle.

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Chapter 1 to 3 summary

  • 1. Everything you ever needed to know about everything
  • 4.
  • 5. Carbohydrates Starch, Glycogen & Cellulose Sucrose, Lactose Glucose, Fructose
  • 6. Types of Lipids Triglycerides Phospholipids
  • 7. The nucleotide monomer PHOSPHATE SUGAR Nucleic acids contain C, H & O in addition to N & P
  • 8. Proteins 3 groups: Amino group, Carboxyl group & R-group When joined together they form peptide bonds Aside from the C, H, O & N in the base molecule, the R group may also contain S & P
  • 9. Protein structure Primary structure The linear sequence of amino acids Secondary structure The type of peptide bond determines how sections of the protein fold – spiral helix / pleated sheet / random coils Shape reinforced by additional H bonds Tertiary structure Eventual 3D shape formed by folding Shape reinforced by additional H bonds Quanternary structure When a protein is formed by the interaction of 2 or more polypeptide chains
  • 11. Elements of a plasma membrane carbohydrate chains glycolipid glycoprotein phospholipid bilayer protein channel cholesterol
  • 12. How does it regulate molecular transport? Passive Transport Diffusion Osmosis Facilitated diffusion (channel, receptor & carrier mediated) Active Transport Primary (uses chemical energy) Secondary (uses electrochemical gradient)
  • 15.
  • 16.
  • 17. Mitochondrion Energy supplying organelle Produces chemical energy in the form of adenosine triphosphate (ATP) through the process of cellular respiration Outer & inner membrane The inner membrane contains many folds to provide a larger surface area for energy production ATP produced by reactions on inner membrane Only in eukaryotes
  • 20. Enzyme Structure Enzymes have an active site and a regulatory region The active site (formed by folds in the protein) is where substrate binds to the enzyme The regulatory region is where cofactors coenzymes or enzyme inhibitors can alter the function of an enzyme Substrate Products Active site Enzyme inhibitor Regulatory region
  • 21. Factors Affecting Enzyme Activity pH Temperature Pressure Enzyme Concentration Substrate concentration Cofactors / coenzymes Inhibition
  • 22. A simplified version of photosynthesis
  • 23. Light Dependent Reaction
  • 24. Light Independent Reaction
  • 25. Light-dependent reaction Occurs in the grana Light energy is used to split water in to two H+ ions and O2 gas The O2 is released as waste With the power of the two free electrons One H+ ion fuses ADP to Pi to form ATP One H+ ion fuses to NADP to form NADPH
  • 26. Light-independent stage Occurs in Stroma Does not need light, but NADPH and ATP from previous stage Needs CO2 and H+ions Sugar molecules are synthesised from CO2 CO2 = oxidised state (low E compound) C(H2O)n = reduced state (high E compound) NADPH (carrier H+) is the reducing agent ATP is the energy source
  • 27. C3 PLANTS C4 PLANTS
  • 28. Putting Photosynthesis together 2 x PGAL = fructose fructose = glucose fructose + glucose = sucrose glucose x ∞= starch
  • 30. Glycolysis Occurs in cytosol – uses enzymes and vitamins as coenzymes 1 glucose (6C) converted to 2 pyruvate (3C) Forms 2 ATP & 2 NADH
  • 31. Krebs Cycle Occurs in mitochondria Pyruvate initially broken down in to CO2 and Acetyl-coA Joins with 4C molecule to form 6 C molecule CO2 to form 5 C molecule, then again to form 4 C molecule Further oxidation takes place to reform original 4C Throughout cycle, constant oxidation is fusing hydrogen to carrier molecules NAD -> NADH and FAD -> FADH2
  • 32. Electron Transport Occurs in inner membrane of mitochondria Produces 2-3 ATP per loaded receptor Electrons passed from one cytochrome to next until accepted by O2- to form water Return of released protons through ATP synthase carrier provides energy to produce ATP from ADP & Pi (phosphorylation)
  • 33.
  • 34. Anaerobic respiration (in humans) Occurs in muscles where oxygen supply exceeds demand The only stage that can occur is glycolysis So 1 glucose produces 2 ATP 2 NADH convert pyruvate to lactate (lactic acid) Lactate build up causes pH to fall and pain & muscle fatigue When activity returns to normal and oxygen becomes available, lactate converted back to pyruvate to enter the Krebs Cycle.
  • 35. Anaerobic respiration (in yeast) Anaerobic respiration in yeast is called fermentation Pyruvate is broken down in to CO2 and ethanol (alcohol)