Histology Lab Slides

1. Epithelial Tissue
• Characteristics:

2. Simple (= single layer)
Cuboidal
• Description: Single
layer of cube-
shaped cells.
• Location: small
ducts of
glands, kidney Cell is as wide as it is high. One
central nucleus
tubules, surface of
ovary.
• Function: secretion
& absorption

3. Simple
Cuboidal Lumen:
Inside space of
• Cells closely packed Tubular structure
together and touching
one another
• Has a basal surface Nucleus
Apical
Surface
and apical surface Basal
Surface
• Avascular
• Sits on a basement
membrane (difficult to
see on slide)

4. Non- Ciliated Simple Columnar
• Description: Single
layer of non-ciliated
rectangular cells;
contain goblet cells
and sometimes
microvilli.
• Location: GI
tract, ducts, gallbladd
er
• Function: secretion
and absorption

5. • Nuclei lie near base of cell
Simple Columnar Cells
• Goblets secrete mucous at
apical surface to lubricate Goblet Cell
lining of Nucleus
GI, respiratory, reproductiv
e and urinary systems.
• Microvilli increase surface
area for absorption.
Goblet Cell

6. Pseudostratified Ciliated Columnar
Epithelium
• Appears stratified
because nuclei are at
varying levels.
• Ciliated lines airways
of upper respiratory
tract.
• Mucus from Goblets Goblet Cells
traps foreign
particles and are
swept away by cilia.

7. Goblet Cells secreting mucus
Cilia
Nuclei at varying levels

8. Epithelial and
1
Connective
• 1 – pseudostratified
columnar epithelial 2
• 2- simple cuboidal
epithelial 3
• 3- hyaline cartilage
connective tissue

9. Collagen Fiber
(thicker pink ones)
Loose Areolar GROUND SUBSTANCE
Connective Tissue
FIBER
MATRIX
Fibroblast CELLS
Elastic Fiber

10. Teased Dense Regular Connective Tissue
• Forms tendons and
ligaments and
aponeuroses.
• Matrix looks white
and shiny.
• Mostly collagen
fibers with
fibroblasts between
rows.
• Strong attachment!

11. Teased Dense
Regular CT
(magnified)
Fibroblasts
Fibers are
mostly
Collagen.

12. Reticular
Connective Tissue Reticular Cells
• Network of interlacing
reticular fibers & Blue staining
reticular cells Reticular fibers
• Found in stroma
(framework) of
liver, spleen, lymph
nodes, red bone
marrow
• Acts as a filter in spleen
and lymph nodes.

13. Reticular Connective Tissue
• Remember that
connective tissue is
vascular. (Avascular
epithelial tissue
depends on diffusion
of nutrients and
waste through blood Blood vessel:
Single layer of
vessels in CT. Epithelial cells
forms vessel tube.

14. Reticular 1
CT 3
• 1- Reticular 2
cells
• 2- Blood
vessel
• 3- Reticular
fibers

15. Hyaline
Cartilage
• Often called gristle
• Bluish white smokey chondrocytes
looking ground
substance
• Numerous chondrocytes
• Location: ends of long Ground S
bones, ribs, nose, trache
a, larynx, bronchi
• For flexibility and
support
• Most abundant type of
cartilage in body

16. Elastic Cartilage
• Chondrocytes are
located within
threadlike network
of elastic fibers
• Location: Epiglottis
of larynx, external
ear, Eustachian
tubes
• For support and
shape

17. Elastic vs Hyaline Cartilage
Lacuna
(hollow space
holding chondrocyte)
chondrocyte
Smoother
Appearance
Of ground
Elastic fibers throughout
substance
Ground substance

18. Adipose
Tissue
• Adipocytes, “signet
ring” cells with
peripheral nuclei.
• For storage of
triglycerides, to
reduce heat loss, and
serve as an energy nucleus
reserve.
• Location: under
skin, around heart &
kidneys, yellow “signet ring”
marrow, behind adipocyte
eyeball

19. Osseous Tissue (Bone)
• Compact bone consists of
osteons (haversion
systems) that contain:
– Lamellae
– Lacuna
– Osteocytes
– Canaliculi
– Central (haversion)canals
• Spongy bone consists of
thin plates called
trabeculae filled with red
marrow

20. Lamellae
Central(Haversion) Haversion
Canal System
Lacunae

21. Lacunae (where osteocytes reside)
Canaliculi: small
Tunnels where osteocyte
processes run for diffusion
of nutrients and wastes

22. Skeletal Muscle
Tissue
• Striated
– Proteins
• Actin
Striations
• Myosin
• Multinucleated
• Voluntary
• Attach to bones
• For
movement, communicatio
n, posture, heat
production

23. Skeletal muscle fiber
with multiple nuclei

25. Smooth (Visceral) Muscle Tissue
• No striations
• Spindle-shaped
• Single centrally located
nucleus
• Nucleus is long and cigar
shaped
• Involuntary
• Viscera
• Walls of hollow structures
– Blood vessels
– Airways to lungs
– Stomach
– Intestines
– Gallbladder
– Urinary bladder

26. Cigar shaped nuclei

27. Smooth Muscle

28. Cardiac Muscle
Tissue
• Striated, mono-
nucleated, branching
• Self excitatory
• Intercalated discs Intercalated disc
attach fibers together
and contain gap
junctions (help with
nucleus
conduction of
electrical signals)

29. Name the tissues and structures you see.

30. Simple Squamous Epithelial

31. Stratified Squamous Epithelial
These keratinized epithelial cells will
Be sloughed off.
Apical Layer
Basal Layer