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The Cell Membrane and
  Transport Systems
The Cell Membrane
   The boundary of all living cells is a cell
    membrane which controls the entry of
    dissolved substances into and out of the
    cell.
   A cell membrane consists of both lipid and
    protein.
   The membrane is formed of a double –
    layer of lipids.
   Proteins are embedded in this layer
    forming channels that allow water –
    soluble substances pass through
The Cell Membrane
   Phospholipid bi-layer
The Cell Membrane
Crossing The Membrane
    All cells must be able to take in &
     expel various substances in order to
     survive, grow & reproduce.

    As a cell membrane only allows
     some dissolved substances to cross
     it, it is described as being semi –
     permeable OR partially –
     permeable.
Main Types of Transport
   Passive Transport: diffusion & osmosis
   Active Transport
   Endocytosis and Exocytosis

Passive – movement across membranes
  that does not require any energy

Active – movement across membranes
 that does require energy
Diffusion
   Net movement of particles from a
    region of high concentration to a
    region of low concentration.
Diffusion
   Difference in concentration between
    two regions = concentration gradient
   Diffusion occurs when a gradient
    exists and continues until equilibrium
   Factors can effect the rate of diffusion
     when the concentration gradient is large
     when heat is applied
     when molecules are smaller
     when movement occurs through a
      gaseous membrane
Diffusion
   Water, carbon dioxide and other
    small uncharged particles
Osmosis
   Net movement of a
    solvent, (usually water)
    across a differentially
    permeable membrane from
    a region of low solute
    concentration to high
    solute concentration.

   That is, water molecules
    move from a region of high
    solvent concentration to
    low concentration.
Osmosis
Osmosis in Animal Cells
   Isotonic – when the extracellular fluid
    and intracellular fluids are at equal
    concentrations.

   Hypotonic – when the extracellular
    fluid has a higher water concentration
    than the intracellular fluid.

   Hypertonic – when the intracellular
    fluid has a higher water concentration
    than the extracellular fluid.
Osmosis
   High Water                                                            Low water
 Concentration                                                      concentration means
means the solution                                                     the solution is
  is Hypotonic                                                          Hypertonic
 Loses water by                                                      Gains water by
    osmosis                                                             osmosis



                                                                     Solute molecules cannot
Water molecules
                                                                     pass through the
                                                                     partially permeable
                                     Partially                       membrane
                                    permeable
                                    membrane




                     There is a net movement of water molecules
                     towards the less concentrated side (in terms
                            of water molecules) until water
                               concentrations equalize.
Osmosis in Animal Cells
Osmosis in Animal Cells
Isotonic solution
 No net movement of solvent =
 equilibrium



                            Red blood cell =
                            0.9% saline


                            Solution = 0.9%
                            saline
Osmosis in Animal Cells
Hypotonic solution
 Solvent moves into the cell



                               Red blood cell =
                                 0.9% saline

                               Solution = 0.4%
                                   saline
Osmosis in Animal Cells
Hypertonic solution
 Solvent moves out of the cell into the solution




                                     Red blood cell =
                                       0.9% saline

                                     Solution = 1.2%
                                          saline
Osmosis in Plant Cells
    Different to animal cells because of the cell wall
Osmosis in Plant Cells
Isotonic solution
 No net movement of solvent =
 equilibrium
                       Cell placed in solution
                       that has the same
                       concentration as the cell
                       sap
Osmosis in Plant Cells
Hypotonic solution
 Plant cell placed in solution that has a lower
 concentration than the cell sap

                                 •Water enters the vacuole
                                 •The vacuole expands
                                 •Cytoplasm is pushed
                                 outwards
                                 •‘Turgid’ cell
Osmosis in Plant Cells
Hypertonic solution
 Plant cell placed in solution that has a higher
 concentration than the cell sap


                             •Water exits the vacuole
                             •Vacuole shrinks
                             •Cytoplasm moves inwards
                             •‘Plasmolysis’
Active Transport
   Diffusion is very slow & it moves substances
    down a concentration gradient.

   Many substances are needed by organisms
    in much greater amounts than what can be
    provided by diffusion alone. Additionally,
    some substances need to be accumulated in
    cells against the concentration gradient.

   In order for this to occur, energy must be
    used to move the needed substances across
    the cell membrane.
Active Transport
Active   transport occurs through protein channels.

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Cell membrane

  • 1. The Cell Membrane and Transport Systems
  • 2. The Cell Membrane  The boundary of all living cells is a cell membrane which controls the entry of dissolved substances into and out of the cell.  A cell membrane consists of both lipid and protein.  The membrane is formed of a double – layer of lipids.  Proteins are embedded in this layer forming channels that allow water – soluble substances pass through
  • 3. The Cell Membrane  Phospholipid bi-layer
  • 5. Crossing The Membrane  All cells must be able to take in & expel various substances in order to survive, grow & reproduce.  As a cell membrane only allows some dissolved substances to cross it, it is described as being semi – permeable OR partially – permeable.
  • 6. Main Types of Transport  Passive Transport: diffusion & osmosis  Active Transport  Endocytosis and Exocytosis Passive – movement across membranes that does not require any energy Active – movement across membranes that does require energy
  • 7. Diffusion  Net movement of particles from a region of high concentration to a region of low concentration.
  • 8. Diffusion  Difference in concentration between two regions = concentration gradient  Diffusion occurs when a gradient exists and continues until equilibrium  Factors can effect the rate of diffusion  when the concentration gradient is large  when heat is applied  when molecules are smaller  when movement occurs through a gaseous membrane
  • 9. Diffusion  Water, carbon dioxide and other small uncharged particles
  • 10. Osmosis  Net movement of a solvent, (usually water) across a differentially permeable membrane from a region of low solute concentration to high solute concentration.  That is, water molecules move from a region of high solvent concentration to low concentration.
  • 12. Osmosis in Animal Cells  Isotonic – when the extracellular fluid and intracellular fluids are at equal concentrations.  Hypotonic – when the extracellular fluid has a higher water concentration than the intracellular fluid.  Hypertonic – when the intracellular fluid has a higher water concentration than the extracellular fluid.
  • 13. Osmosis High Water Low water Concentration concentration means means the solution the solution is is Hypotonic Hypertonic Loses water by Gains water by osmosis osmosis Solute molecules cannot Water molecules pass through the partially permeable Partially membrane permeable membrane There is a net movement of water molecules towards the less concentrated side (in terms of water molecules) until water concentrations equalize.
  • 15. Osmosis in Animal Cells Isotonic solution No net movement of solvent = equilibrium Red blood cell = 0.9% saline Solution = 0.9% saline
  • 16. Osmosis in Animal Cells Hypotonic solution Solvent moves into the cell Red blood cell = 0.9% saline Solution = 0.4% saline
  • 17. Osmosis in Animal Cells Hypertonic solution Solvent moves out of the cell into the solution Red blood cell = 0.9% saline Solution = 1.2% saline
  • 18. Osmosis in Plant Cells  Different to animal cells because of the cell wall
  • 19. Osmosis in Plant Cells Isotonic solution No net movement of solvent = equilibrium Cell placed in solution that has the same concentration as the cell sap
  • 20. Osmosis in Plant Cells Hypotonic solution Plant cell placed in solution that has a lower concentration than the cell sap •Water enters the vacuole •The vacuole expands •Cytoplasm is pushed outwards •‘Turgid’ cell
  • 21. Osmosis in Plant Cells Hypertonic solution Plant cell placed in solution that has a higher concentration than the cell sap •Water exits the vacuole •Vacuole shrinks •Cytoplasm moves inwards •‘Plasmolysis’
  • 22. Active Transport  Diffusion is very slow & it moves substances down a concentration gradient.  Many substances are needed by organisms in much greater amounts than what can be provided by diffusion alone. Additionally, some substances need to be accumulated in cells against the concentration gradient.  In order for this to occur, energy must be used to move the needed substances across the cell membrane.
  • 23. Active Transport Active transport occurs through protein channels.