The 2nd Gulf Andrology Conference Riyadh Military Hospital, Ministry of Defense Riyadh, Saudi Arabia, March 3-4, 2012 Lectures: Current and Future Treatments for Azoospermia

8. Sperm!
Detection!
Rate!
FNA Sites/Testicle!

10. Finding Sperm in Nonobstructive Azoospermia
Microdissection FNA Mapping

14. “Directed”
TESE
Sperm Present
<2 sites >2 FNA All FNA
sperm/map sites/map sites/map
TESE TESA
(n=29) (n=38)

18. What Exactly is Metabolomics? !!

19. In the testis, somatic cells
are metabolically
“quiet” compared to
rapidly dividing germ
cells!
Analogy: A pile of bricks
along a desert road-
indicates something is
being built.!

20. The Metabolomic Signature of a Testicle
Phosphocholine (PC)!
Glycerophosphocholine (GPC)! Total Choline!
Choline!
Normal/Fertile !
Aaronson et al. !
Creatine! Hum Reprod. 2010!
Alanine! Lactate!
Azoospermic Men
with Sertoli Cell
Only !

21. Aaronson et al. Hum Reprod.
2010

28. Leydig cells!
Stromal cells!
Sertoli cells!
Fibronectin!