1. STS Conditioner
A Developmental Product from Sustainable Technology Systems
• STS Biomass Conditioner is a chemical solution that reduces into an
environmentally safe product.
• STS Conditioner eliminates/ reduces the use of biofuels industrial antibiotics
that are polluting our waterways
• STS Conditioner softens the lignin into liquid and/or gel depending on the
feedstock thereby exposing more surface area for enzymatic action. This
saves time and money in mashing and shredding biomass.
• STS Conditioner eliminates invasive bacteria that will compete for glucose
after starch reduction. Glucose is the basic sugar that forms alcohol.
• STS Conditioner has proven to produce over 8.5 percent alcohol increase
per batch (even 1% is significant to industry). Greater yields are anticipated
after completing this study.
• STS Conditioner reduces the cost of enzymes which is one of the most costly
chemical purchases for the biofuels producer.

2. STS Conditioner
A Developmental Product from Sustainable Technology Systems
• STS Conditioner eliminates offensive odors. Cattail rhizomes are covered
with a bacterial biofilm which cannot be scrubbed off or killed with low
heat. Once the gross bioburden is removed (dirt or mud) STS conditioner
eliminates the biofilm.
• STS Conditioner is bacteriocidal and it promotes healthy yeast growth.
• STS Conditioner is significantly lower in price than Lactrol and Beta
Hops.
Cara Mullen, MPH Peggy Korth
Sustainable Technology Systems Sustainable Technology Systems
108 W. 22nd 40 Sun Valley Dr.
Hutchinson, KS 67502 Spring Branch TX 78070
www.sustainabletechsys.com FAX 820 885-4827
Cell: 620-960-6114 rpk@gvtc.com

3. Studies of antibacterial effect of STS Conditioner with corn
mash samples from corn ethanol plants.
• The goal was to find the lowest concentration of STS CONDITIONER
that will kill bacteria but not impede the growth or fermentation
efficiency of the yeast.
• We performed studies with the corn mash sample from Prairie Horizon.
– The corn mash sample was taken prior to the prop so we needed to add
yeast.
– Before adding anything we tested for growth and found two populations
that seem to be bacteria and a fungus.
– The latter has a phenotype that is distinctly different from bacteria and
S. cerevisiae.
• We determined values for MIC of STS CONDITIONER for the bacteria and
the other contaminant in this sample to be 0.05 and 0.5, respectively.
• We added yeast and STS nutrients and determined the MIC of STS
CONDITIONER for the yeast.
• The results show that STS CONDITIONER impacts yeast propagation at
levels of 0.5% and above, even in the presence of STS nutrients.

4. Studies of antibacterial effect of STS Conditioner with corn
mash samples from corn ethanol plants.
• Following this series we spiked a sample with 2 x 106 cells of bacteria
(lactobacillus fermentum) and ran yeast (Saccharomyces cerevicea)
propagation trials in the presence of various concentrations of STS
Conditioner, Lactrol, and Isostab.
• The concentrations of Lactrol and Isostab were chosen based on the
recommended values (Lactrol 0.5 ppm, Isostab 50 ppm) in the product
literature.
• Controls involving yeast only and bacteria only were also included. The
concentrations of the test solutions were:
STS 0.5 % 0.1% 0.05%
Conditioner (5,000 ppm) (1,000 ppm) (500 ppm)
Lactrol 1 ppm 0.5 ppm 0.25 ppm
Isostab 100 ppm 50 ppm 25 ppm

5. Studies of antibacterial effect of STS Conditioner with corn
mash samples from corn ethanol plants.
• Total number of baffled flasks - 12
• Final volume in flasks: 50ml = 40ml (w/v) corn mash + 10ml dewater
• pH of the corn mash + yeast samples was measured before and after
addition of the test solutions, and also at 3 subsequent time points for all
samples
• Ave initial pH 5.9 for all samples before the addition of test solutions
• pH for Lactrol and IsoStab decreased on average to pH 5.3
• pH for STS Conditioner @ 0.50% increased 4.58 to 5.3
@ 0.10% increased 5.23 to 5.3
@ 0.05% decreased 5.78 to 5.35

6. Studies of antibacterial effect of STS Conditioner with corn
mash samples from corn ethanol plants.
• For the case of Lactrol addition to samples containing yeast and bacteria,
photos of the plates at the three Lactrol concentrations were shown.
• Figure 1, show a slight decrease in the colonies (presumably a decrease in
bacterial colonies) as a function of Lactrol concentration.
– Little or no variation was observed for Isostab in a similar trial.
• For the case of STS Conditioner addition to samples containing yeast and
bacteria, photos of the plates at the three STS Conditioner concentrations
are shown in Fig 2.
Figure 1 Figure 2

7. Studies of antibacterial effect of STS Conditioner with corn
mash samples from corn ethanol plants.
• Qualitatively, from the growth on the plates it is visually apparent that
STS Conditioner at 0.5% is more effective at killing the bacteria than
Lactrol at 1 ppm under these conditions.
– However, when both the yeast and lactobacillus are present at high numbers so it is
difficult to distinguish them and make quantitatively accurate counts.

8. STS Conditioner softens Lignins
Applied to Cellulosic Scrap: No heat, 1 week exposure

9. STS Conditioner softens Lignins
Applied to Shredded computer paper: No heat, 90 second exposure