Influence of grape genotype, plant growth stage, and geographic location

1. Biological control of Pierce’s disease: Influence of grape genotype, plant growth stage, and geographic location D. L. Hopkins Mid-Florida REC University of Florida Phil Brannen Plant Pathology Dept. University of Georgia

3. Current tests on new grape plantings of various grape genotypes Field injections

4. Greenhouse injection

6. Effect of strain EB92-1 on PD in various cultivars in Central Florida

8. Inoculated approximately 10 7 cells EB92-1 per plant into 2 nd and 3 rd internodes. Mature Plant Treatments

9. Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a high incidence of PD

10. Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in North Georgia vineyard 29 36 10 6

11. Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a low incidence of PD

12. Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in a North Georgia vineyard 3 4 (2)

13. Planned Mature Plant Study for 2009 Inject EB92-1 into the main trunk of the vines to get more systemic affect

14. North Georgia Central Florida North Carolina South Carolina Different Locations (Climates)

15. Effect of EB92-1 on development of PD in new plantings in Florida (4 years - Cabernet Franc, Chardonnay, Chenin Blanc, Golden Muscat, Ruby Cabernet), Georgia (1 year – Cabernet Sauvignon, Cab. Franc, Vidal Blanc), S. Carolina (1 year – Neptune)

16. Comparison of treatment method with EB92-1 on control of Pierce’s disease in rooted cuttings in the greenhouse. b b a b a a EB92-1 Treatment Methodology

18. 10 5 PD92-9 cells Colonization of host plant by EB92-1 and pathogen (PD92-9 10 5 PD92-9 cells

20. Thanks!