1. SOUTHERN BLOT
By: Kelsey Martin

2. WHAT IS SOUTHERN BLOT?
 a method routinely used in molecular biology for
detection of a specific DNA sequence in DNA
samples

3. ABOUT SOUTHERN BLOT
 Named after Edward M. Southern
 Developed at Edinburgh University in the 1970s
 Designed to locate a particular sequence of DNA
within a complex mixture

4. USES FOR SOUTHERN BLOTTING
 Gene discovery and mapping
 Evolution and development studies
 Diagnostics and Forensics
 As a definitive test to ensure that a particular
section of DNA has been successfully incorporated
into to genome of the host organism

5. TECHNIQUE
 DNA is restricted with enzymes
 DNA is loaded into a well of the gel matrix
 An electric current is passed through the gel
 DNA moves away from the negative electrode
 DNA is transferred out of the gel and onto a
membrane
 A labeled probe specific for the gene in question is
incubated with the blot.
 The blot is washed to remove non-specifically
bound probe
 A development step allows visualization of the DNA
that is bound