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Cellular Respiration Stage 4:   Electron Transport Chain 2006-2007
Cellular respiration
2006-2007 What’s the point? The point is to make ATP ! ATP
ATP accounting so far… ,[object Object],[object Object],[object Object],A working muscle recycles over  10 million ATPs per second There’s got to be a better way! I need a lot more ATP !
There  is  a better way! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],That sounds more like it !   O 2
Mitochondria  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Oooooh ! Form fits  function !
Electron Transport Chain Intermembrane space Mitochondrial matrix Q C NADH  dehydrogenase cytochrome bc  complex cytochrome c oxidase complex Inner mitochondrial membrane
Remember the Electron Carriers?  G3P Glycolysis Krebs cycle 8 NADH 2 FADH 2 2 NADH Time to break open the piggybank ! glucose
Electron Transport Chain intermembrane space mitochondrial matrix inner mitochondrial membrane NAD + Q C NADH  H 2 O H + e – 2H +  +  O 2 H + H + e – FADH 2 NADH  dehydrogenase cytochrome bc  complex cytochrome c oxidase complex FAD e – H H    e- + H + NADH    NAD +  + H H Building proton gradient ! What powers the proton (H + ) pumps?… 1 2 p e
Stripping H from Electron Carriers ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],ATP ADP + P i TA-DA !! Moving electrons do the work ! H + H + H + H + H + H + H + H + H + H + H + H + NAD + Q C NADH  H 2 O H + e – 2H +  +  O 2 H + H + e – FADH 2 1 2 NADH  dehydrogenase cytochrome   bc  complex cytochrome c oxidase complex FAD e –
But what “pulls” the  electrons down the ETC? electrons flow downhill  to O 2 oxidative phosphorylation O 2 H 2 O
Electrons flow downhill ,[object Object],[object Object],[object Object],[object Object],make ATP instead of fire !
We did it! ,[object Object],[object Object],[object Object],[object Object],ATP Are we there yet? “ proton-motive” force H + ADP + P i H + H + H + H + H + H + H + H +
[object Object],[object Object],Chemiosmosis Chemiosmosis  links the Electron Transport Chain to ATP synthesis So that’s the point !
Peter Mitchell ,[object Object],[object Object],1961  |  1978 1920-1992 proton motive force
H + H + O 2 + Q C ATP Pyruvate from cytoplasm Electron transport system ATP synthase H 2 O CO 2 Krebs cycle Intermembrane space Inner mitochondrial membrane 1.  Electrons  are harvested and carried to the transport system. 2.  Electrons  provide energy  to pump  protons  across the membrane. 3.  Oxygen  joins  with  protons  to form  water . 2H + NADH NADH Acetyl-CoA FADH 2 ATP 4.  Protons  diffuse back in down their concentration gradient, driving the  synthesis of  ATP . Mitochondrial  matrix 2 1 H + H + O 2 H + e - e - e - e - ATP
Cellular respiration 2 ATP 2 ATP ~36 ATP + + ~40 ATP
Summary of cellular respiration ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],C 6 H 12 O 6 6O 2 6CO 2 6H 2 O ~40 ATP  + + +
Taking it beyond… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],O 2 ,[object Object],NAD + Q C NADH  H 2 O H + e – 2H +  +  O 2 H + H + e – FADH 2 1 2 NADH  dehydrogenase cytochrome   bc  complex cytochrome c oxidase complex FAD e –
 
2006-2007 What’s the point? The point is to make ATP ! ATP
Respiratory substrates ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
THE RESPIRATORY QUOTIENT  ,[object Object],[object Object],[object Object]
Questions ,[object Object],[object Object],[object Object]
THE RESPIRATORY QUOTIENT  ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
RQ ,[object Object],[object Object],[object Object]
RQ ,[object Object],[object Object],[object Object],[object Object],[object Object]
RQ ,[object Object],[object Object]
RQ ,[object Object],[object Object],[object Object],[object Object]
RQ ,[object Object]

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Chapter 15 lecture 4

  • 1. Cellular Respiration Stage 4: Electron Transport Chain 2006-2007
  • 3. 2006-2007 What’s the point? The point is to make ATP ! ATP
  • 4.
  • 5.
  • 6.
  • 7. Electron Transport Chain Intermembrane space Mitochondrial matrix Q C NADH dehydrogenase cytochrome bc complex cytochrome c oxidase complex Inner mitochondrial membrane
  • 8. Remember the Electron Carriers? G3P Glycolysis Krebs cycle 8 NADH 2 FADH 2 2 NADH Time to break open the piggybank ! glucose
  • 9. Electron Transport Chain intermembrane space mitochondrial matrix inner mitochondrial membrane NAD + Q C NADH H 2 O H + e – 2H + + O 2 H + H + e – FADH 2 NADH dehydrogenase cytochrome bc complex cytochrome c oxidase complex FAD e – H H  e- + H + NADH  NAD + + H H Building proton gradient ! What powers the proton (H + ) pumps?… 1 2 p e
  • 10.
  • 11. But what “pulls” the electrons down the ETC? electrons flow downhill to O 2 oxidative phosphorylation O 2 H 2 O
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. H + H + O 2 + Q C ATP Pyruvate from cytoplasm Electron transport system ATP synthase H 2 O CO 2 Krebs cycle Intermembrane space Inner mitochondrial membrane 1. Electrons are harvested and carried to the transport system. 2. Electrons provide energy to pump protons across the membrane. 3. Oxygen joins with protons to form water . 2H + NADH NADH Acetyl-CoA FADH 2 ATP 4. Protons diffuse back in down their concentration gradient, driving the synthesis of ATP . Mitochondrial matrix 2 1 H + H + O 2 H + e - e - e - e - ATP
  • 17. Cellular respiration 2 ATP 2 ATP ~36 ATP + + ~40 ATP
  • 18.
  • 19.
  • 20.  
  • 21. 2006-2007 What’s the point? The point is to make ATP ! ATP
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27.
  • 28.
  • 29.
  • 30.
  • 31.

Notes de l'éditeur

  1. Oxidation refers to the loss of electrons to any electron acceptor, not just to oxygen. Uses exergonic flow of electrons through ETC to pump H+ across membrane.
  2. Pumping H+ across membrane … what is energy to fuel that? Can’t be ATP! that would cost you what you want to make! Its like cutting off your leg to buy a new pair of shoes. :-( Flow of electrons powers pumping of H + O 2 is 2 oxygen atoms both looking for electrons
  3. Electrons move from molecule to molecule until they combine with O & H ions to form H 2 O It’s like pumping water behind a dam -- if released, it can do work
  4. Chemiosmosis is the diffusion of ions across a membrane. More specifically, it relates to the generation of ATP by the movement of hydrogen ions across a membrane. Hydrogen ions (protons) will diffuse from an area of high proton concentration to an area of lower proton concentration. Peter Mitchell proposed that an electrochemical concentration gradient of protons across a membrane could be harnessed to make ATP. He likened this process to osmosis, the diffusion of water across a membrane, which is why it is called chemiosmosis.
  5. Where did the glucose come from? from food eaten Where did the O 2 come from? breathed in Where did the CO 2 come from? oxidized carbons cleaved off of the sugars (Krebs Cycle) Where did the CO 2 go? exhaled Where did the H 2 O come from? from O 2 after it accepts electrons in ETC Where did the ATP come from? mostly from ETC What else is produced that is not listed in this equation? NAD, FAD, heat!
  6. What if you have a chemical that punches holes in the inner mitochondrial membrane?