Project: AFM test towards investigation of neurons and neuronal microtubules.

1. Project: AFM test
towards investigation Home Page
of neurons and Title Page
neuronal microtubules
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Presented in PosLab (C368) on May 30th, 2007, at 15:15
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by Mikael O. Bonnier
for the course Experimental Biophysics, TNF030 Quit
with advisor Lars Henrik Dæhli Skjolding

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Chip with interdigitated electrodes, but these are pictures of the Go Back
chip connections in the micrometer range in optical and AFM
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Three contact mode cantilevers, a single intermittent contact
mode cantilever, and three intermittent contact mode can- Close
tilevers, respectively on one chip each.
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Adjusting laser beam and detector. Full Screen
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AFM image using worn tip. Note the missing data. Quit

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The advanced optical microscope (Nikon TE2000-U) with the
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AFM mounted.

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Active vibration damper (Halcyonics Micro 40). Close
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Images of the marker pen cross in water using optical Go Back
microscope and AFM, respectively. Note the missing data.
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BioCellTM . Close
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Stem cells using x40 microscope lens. Close
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Invading bacteria using x10 microscope lens. Close
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More differentiated stem cells in optical directly overlayed with
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AFM height images (by Dr Rachel Owen at JPK Intruments
demonstrating the AFM in our lab). Quit

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Conclusions
• Study the manuals and control theory Contents
• Better if prism were made so that it could only fit one way
into the AFM
• Many interesting experiments, that may require stem cells ge-
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netically modified so that they have different microtubule hair
lengths, can be made using our new AFM Go Back
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Acknowledgements Contents
• Lars Henrik Dæhli Skjolding
• Jan Tønnesen
• Jonas Tegenfeldt Page 14 of 15
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Happy Summer! Quit