dr lu8b

1. Not normally isolateable
Usually gram variable

2.  L. pneumophila is the prototype bacterium of
this group
 Fastidious, aerobic and stains poorly with
gram stain
 Basic fuchsin must be used as a counterstain
 Can be grown in buffered charcoal-yeast
extract (BCYE), pH 6.9, 35 degrees & 90%
humidity
 Catalase positive & oxidase positive

3.  Ubiquitous in warm and moist environment
 Can multiply in free living amoebas and
coexist with them in biofilms
 Contamination from inhalation from
airconditioning systems, shower heads
 Causes pneumonia and lesions within
phagocytic cells
 Susceptible to erythromycin and other drugs
such as Rifampin

4.  B. bacilliformis – causes Oroya fever
 B. quintana – causes trench fever
 B. henselae – Cat scratch fever
 Pleaomorphic and slow growing, difficult to
isolate in the laboratory
 Can be seen in infected tissues stained with
Warthin-Starry silver impregnation stain

5.  Caused by Bartonella, is a disease
predominantly of immunosuppressed
individuals, particularly AIDS patients
 Present as an enlarging red ( cranberry like )
papule, often with surrounding scale and
erythma
 Clinical appearance similar with Kaposi’s
sarcoma, form of the disease is called
peliosis hepatis

6.  Serologically distinct organism isolated in the
female genitourinary tract and associated
with vaginosis ( no inflammatory cells )
 In wet smears, the bacterial vaginosis yields
clue cells ( vaginal epithelial cells covered
with many gram variable bacilli
 Vaginal discharge often have a strange fishy
odor with pH over 4.5
 Drug of choice is oral metronidazole

7.  Motile, curved, gram variable, anaerobic
rods isolated from “bacterial vaginosis”
 Possibly part of the vaginal normal anaerobic
flora
 The organisms are most commonly detected
but grow with difficulty in aerobic cultures

8.  Aerobic, highly pleomorphic organism that
forms irregular chains of bacilli interspersed
with fusiform enlargements and large round
bodies
 Mesophilic, in media with serum, protein,
egg yolk, starch & ceases to grow at 22 C
 Normal inhabitants of rat throats, and
humans can be infected with rat bites
characterized by septic fever, petechial
rashes, and a very painful polyarthritis

9.  C. granulomatis, related to Klebsiella and
causes granuloma inguinale, an uncommon
STD characterized by genital ulcers.
 Hard to culture even with media enriched
with egg yolk
 Effective treatment with ampicillin and
tetracycline
 CausesWhipples disease, stain with Periodic
Acid Schiff Stain ( PAS )