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Releasing Incisions Using Upward-Motion
Scissors Technique for Flap Mobilization
for Guided Bone Regeneration or
Periodontal Surgery:
Technical Introduction and a Case Report
Int J Periodontics Restorative Dent 2018
Authors - Mitsuharu Inoko, Satoko Rubin, Yoshihiro, Atsushi Saito
PRESENTATION BY – DR. MD ABDUL HALEEM
• Introduction
• Materials and Methods
– Flap Advancement Using the UMST
– Clinical Case
• Discussion
• Conclusion
• References
INTRODUCTION
• A critical phase for guided bone regeneration or periodontal surgery is
the closure of the grafted areas with a tension-free adaptation of the
mucoperiosteal flap.
• Before making periosteal releasing incisions, it is necessary to
understand the anatomy related to the periosteum and the course of
blood vessels supplying the alveolar mucosa and gingiva.
• If primary closure is not achieved with a tension-free
flap adaptation, impaired healing may result from
flap dehiscence and wound exposure during the
healing process leading to in a suboptimal outcome
in regenerated bone volume.*
• The periosteum consists of an inner layer composed of
osteoblasts surrounded by osteoprogenitor cells and an
outer layer rich in blood vessels and nerves and composed
of collagen fibers and fibroblasts.*
• Blood supply for the gingiva and periosteum comes from
supraperiosteal vessels which run roughly parallel to the long
axis of teeth that branches and subdivide in the lamina
propria of the gingiva and form the vascular network on the
periosteum.*
• When full-thickness flaps are raised, releasing incisions that
extend deep into the underlying periosteal tissue should be
avoided to minimize damage to the microvasculature and
nerves.
• The traditional flap design to attain tension-free primary closure
involves two vertical releasing incisions in combination with a
periosteal releasing incision.*
• This is a straightforward and predictable method that can be
performed by most of the experienced clinicians.*
• However, these releasing incision in shallow areas sometimes
cannot achieve a complete passive closure of the flaps.
• To overcome this, a different approach to releasing incision
is needed.
• The aim of this article is to introduce the “upward-motion scissors technique” (UMST), which
involves creation of multiple shallow superficial releasing incisions in 1-mm intervals and
provides complete passive primary closure for safe and predictable periodontal/bone
regeneration therapy.
MATERIALS AND METHODS
• After local anesthesia is administered, a broad full-
thickness mucoperiosteal flap is elevated.
• When working near the mandibular premolar
region, the mental foramen is marked and the
mental nerve is isolated.
• Following vertical incision, a series of releasing
incisions are made with the UMST.
Flap Advancement Using the UMST:
Upward motion Scissors technique
• With UMST, it is important to always avoid the incision site
where the thickness is very thin.
• Otherwise, necrosis of the flap may result due to compromised
blood supply and chewing forces in the area.
• Flap thickness can be confirmed visually by observing the
longitudinal section of the flap at vertical incision.
• The incision is started by holding the flap straight up with a
hemostat.
• A pair of scissors (preferably Goldman Fox #1) is then inserted
between the periosteum and the underlying soft tissue.
• The periosteum is incised with upward motion using the scissors, lifting the layer of
periosteum to avoid damage to the vessels and nerves around the surgical field.
• After the incision has been made, it is always necessary to go back to the incision line
with the blunt side of the scissors to make sure the thin periosteal fibers are not left
behind. This confirmation will maximize the release of every incision.
• The subsequent incision starts 1mm apical from the first one and the same technique is
repeated here.
• Once adequate release of the flap is obtained by multiple incisions with the UMST, flaps
are gently advanced and sutured with horizontal mattress and interrupted sutures to
secure the closure.
Clinical Case:
• A systemically healthy 42-year-old woman presented with a chief
complaint of masticatory disturbance and deviation of the jaw to
the left after losing the left mandibular molars.
• The bone defect was a combination of horizontal and vertical
atrophy with the horizontal being more significant.
• The patient elected to have implant treatment and the following
treatment plan was proposed and accepted by the patient.
• One hour before the surgery, amfenac sodium (25 mg) and
bacampicillin hydrochloride (750 mg) were administered.
• The patient rinsed her mouth with 0.025% benzalkonium chloride
solution prior to the surgery.
• After administration of local anesthesia with Xylestesin,
incisions including a vertical incision were made and a
full-thickness flap was elevated.
• The mental foramen was marked and the mental nerve
was isolated.
• Releasing incisions were made with the UMST.
• Following confirmation of adequate flap release,
decortication of the surrounding bone was performed.
• Three Osseotite NT implants were placed with an
insertion torque of 50 Ncm.
• While preparing the osteotomy using Quad Shaping
Drills, autogenous bone was harvested.
• An 18 × 25-mm Titanium Mesh was customized to cover
the buccal dehiscence about 8 mm in height with the
expectation that the mesh would allow for 3 mm of
horizontal bone regeneration.
• Harvested autogenous bone was placed to cover the
exposed implant surface and then deproteinized bovine
bone mineral (Bio-Oss) was used to fill the rest of the
space in the mesh.
• The mesh was stabilized with customized cover screws
to stabilize the bone graft material.
• Before the flaps were closed, a collagen membrane
(Ossix 25 × 30 mm) was placed over the mesh.
• The wound was completely closed with passive
flaps achieved by the UMST.
• Horizontal mattress and interrupted sutures with
5-0 Monocryl were used to secure the closure of
the flaps.
• Postoperative antibiotics (Bacampicillin
hydrochloride 250 mg every 8 hours for 7 days) and
analgesics (amfenac sodium 25 mg, as needed)
were given along with postoperative instructions.
• At 9 months after the surgery, the second-stage surgery was performed to expose the
implants.
• Upon removal of the titanium mesh, excellent bone regeneration was noted.
• A large defect initially 8 mm in size was completely covered with 3 mm of horizontal
bone.
• A free gingival graft was performed to augment the zone of keratinized tissue around
the implants.*
• After the healing of the soft tissues,
the final restoration was constructed
and a screw-retained implant-
supported porcelain-fused-to-zirconia
bridge replacing the mandibular left
first and second premolars and first
molar was delivered to the patient
• The 3-year postoperative radiograph
demonstrated the stability of the crestal
bone.
DISCUSSION
• In this report, the authors introduced the UMST for flap advancement and
presented an anatomically challenging case that involved the use of UMST.
• Based on the experiences of more than 100 cases performed over the last 9 years,
the authors recommend this surgical technique, particularly in cases where
potential damage to nerves or blood vessels are of concern.
• Even with an understanding of the critical anatomy, surgical procedures including
releasing incision can be challenging when anatomical abnormalities are present.
• For releasing incision, a single incision can be made with a scalpel instead of
scissors.
• However, it can be difficult to control the depth of the incision with a surgical blade.
• Different approaches for flap advancement to attain tension-free primary closure
have been reported.*
• One example is the use of a buccal periosteal pocket technique.*
• This technique increases soft tissue mobility and elasticity and allows better
support of bone substitute material.
• However, it is necessary to advance a periosteal elevator deep into the
subperiosteal soft tissue area.
• This could still cause injuries to microvasculature and nerves.
• With the UMST, multiple shallow incisions are made using curved scissors with the
tip carefully controlled and facing upward, avoiding potential damage to underlying
tissues.
• Even though most incisions made with the scissors are clean and accurate, some
thin periosteum fibers can be left behind.
• Thus, it is important to go back with the blunt side of the scissors to trace the
incision and identify any tags from the remaining fibers.
• Once the fibers are identified, the scissors should be used to cut them to make sure
the flaps can be fully extended.
• Any curved scissors of appropriate size can be applied to perform the UMST.
• However, based on treatment experience, a preferred choice is Goldman Fox #1.
• Some modifications, such as a slightly longer handle and a duller tip, may greatly
improve its handling and safe operation.
• An area where it is difficult to place a releasing incision is near the maxillary
tuberosity, where the coronoid process is present.
• Due to the limited access to the area, it is extremely difficult to control the incision
with a surgical blade.
• If the incision is too deep in this area, damage to the pterygoid plexus could
occur.*
• Based on the authors’ experience, the UMST can provide easier access to precisely
place 1mm incremental incision lines even in undercut areas of the maxillary
tuberosity.
• With the UMST, the use of a microscope or surgical scope is recommended to
control the angle and depth of the incision and the extension of the flap to further
minimize potential complications.
• Information is still limited on the effects of different incision placements having on
the amount of flap extension.*
• Therefore, clinicians have to predict the amount of flap extension based on their
experiences or assumptions.*
• Further studies are needed to establish safe and predictable releasing incision
design for flap management.
CONCLUSIONS
• Within the limitations of the authors’ experience, the upward-
motion scissors technique (UMST) may prove to be an
effective method to secure flap advancement in areas where a
surgical blade is difficult or unsafe to make releasing incisions.
• More studies are needed to validate the clinical results of the
technique presented here.
REFERENCES
• 01. Greenstein G, Greenstein B, Cavallaro J, Elian N, Tarnow D. Flap advancement: Practical techniques to attain
tension-free primary closure. J Periodontol 2009;80:4–15.
• 2. Machtei EE. The effect of membrane exposure on the outcome of regenerative procedures in humans: A meta-
analysis. J Periodontol 2001;72:512–516.
• 3. Fugazzotto PA. Maintaining primary closure after guided bone regeneration procedures: Introduction of a new flap
design and preliminary results. J Periodontol 2006;77:1452–1457.
• 4. Leong DJ, Oh TJ, Benavides E, Al-Hezaimi K, Misch CE, Wang HL. Comparison between sandwich bone augmentation
and allogenic block graft for vertical ridge augmentation in the posterior mandible. Implant Dent 2015;24:4–12
• 5. Ten Cate AR. Oral Histology: Development, Structure, and Function, ed 2. St Louis; Mosby, 1985.
• 6. Fiorellini JP, Stathopoulou PG. Anatomy of the periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA
(eds). Carranza’s Clinical Periodontology, ed 12. St Louis: Elsevier Saunders, 2015:34.
• 7. Keller GJ, Cohen DW. India ink perfusions of the vascular plexus of oral tissues. Oral Surg Oral Med Oral Pathol
1955;8: 539–542.
• 8. Folke LE, Stallard RE. Periodontal microcirculation as revealed by plastic microspheres. J Periodontal Res 1967;2:53–
63.
• 9. Velvert P, Peters CI. Soft tissue management in endodontic surgery. J Endod 2005;31:4–16.
• 10. Park JC, Kim CS, Choi SH, Cho KS, Chai JK, Jung UW. Flap extension attained by vertical and periosteal-releasing
incisions: A prospective cohort study. Clin Oral Implants Res 2012;23:993–998.
REFERENCES
• 11. Fugazzotto PA. Maintenance of soft tissue closure following guided bone regeneration: Technical
considerations and report of 723 cases. J Periodontol 1999;70: 1085–1097.
• 12. Warrer K, Buser D, Lang NP, Karring T. Plaque-induced peri-implantitis in the presence or
absence of keratinized mucosa. An experimental study in monkeys. Clin Oral Implants Res
1995;6:131–138.
• 13. Schrott AR, Jimenez M, Hwang JW, Fiorellini J, Weber HP. Five-year evaluation of the influence of
keratinized mucosa on peri-implant soft-tissue health and stability around implants supporting
fullarch mandibular fixed prostheses. Clin Oral Implants Res 2009;20:1170–1177.
• 14. Thoma DS, Buranawat B, Hämmerle CH, Held U, Jung RE. Efficacy of soft tissue augmentation
around dental implants and in partially edentulous areas: A systematic review. J Clin Periodontol
2014; 41(Suppl 15):S77–S91.
• 15. Steigmann M, Salama M, Wang HL. Periosteal pocket flap for horizontal bone regeneration: A
case series. Int J Periodontics Restorative Dent 2012;32: 311–320.
• 16. Li J, Xu X, Wang J, Jing X, Guo Q, Qiu Y. Endoscopic study for the pterygopalatine fossa anatomy:
Via the middle nasal meatus-sphenopalatine foramen approach. J Craniofac Surg 2009;20: 944–
947.
Releasing Incisions Using Upward-Motion Scissors Technique for Flap Mobilization for Guided Bone Regeneration or Periodontal Surgery: Technical Introduction and a Case Report.

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Releasing Incisions Using Upward-Motion Scissors Technique for Flap Mobilization for Guided Bone Regeneration or Periodontal Surgery: Technical Introduction and a Case Report.

  • 1.
  • 2. Releasing Incisions Using Upward-Motion Scissors Technique for Flap Mobilization for Guided Bone Regeneration or Periodontal Surgery: Technical Introduction and a Case Report Int J Periodontics Restorative Dent 2018 Authors - Mitsuharu Inoko, Satoko Rubin, Yoshihiro, Atsushi Saito PRESENTATION BY – DR. MD ABDUL HALEEM
  • 3. • Introduction • Materials and Methods – Flap Advancement Using the UMST – Clinical Case • Discussion • Conclusion • References
  • 4. INTRODUCTION • A critical phase for guided bone regeneration or periodontal surgery is the closure of the grafted areas with a tension-free adaptation of the mucoperiosteal flap. • Before making periosteal releasing incisions, it is necessary to understand the anatomy related to the periosteum and the course of blood vessels supplying the alveolar mucosa and gingiva. • If primary closure is not achieved with a tension-free flap adaptation, impaired healing may result from flap dehiscence and wound exposure during the healing process leading to in a suboptimal outcome in regenerated bone volume.*
  • 5. • The periosteum consists of an inner layer composed of osteoblasts surrounded by osteoprogenitor cells and an outer layer rich in blood vessels and nerves and composed of collagen fibers and fibroblasts.* • Blood supply for the gingiva and periosteum comes from supraperiosteal vessels which run roughly parallel to the long axis of teeth that branches and subdivide in the lamina propria of the gingiva and form the vascular network on the periosteum.* • When full-thickness flaps are raised, releasing incisions that extend deep into the underlying periosteal tissue should be avoided to minimize damage to the microvasculature and nerves.
  • 6. • The traditional flap design to attain tension-free primary closure involves two vertical releasing incisions in combination with a periosteal releasing incision.* • This is a straightforward and predictable method that can be performed by most of the experienced clinicians.* • However, these releasing incision in shallow areas sometimes cannot achieve a complete passive closure of the flaps. • To overcome this, a different approach to releasing incision is needed. • The aim of this article is to introduce the “upward-motion scissors technique” (UMST), which involves creation of multiple shallow superficial releasing incisions in 1-mm intervals and provides complete passive primary closure for safe and predictable periodontal/bone regeneration therapy.
  • 7. MATERIALS AND METHODS • After local anesthesia is administered, a broad full- thickness mucoperiosteal flap is elevated. • When working near the mandibular premolar region, the mental foramen is marked and the mental nerve is isolated. • Following vertical incision, a series of releasing incisions are made with the UMST. Flap Advancement Using the UMST: Upward motion Scissors technique
  • 8. • With UMST, it is important to always avoid the incision site where the thickness is very thin. • Otherwise, necrosis of the flap may result due to compromised blood supply and chewing forces in the area. • Flap thickness can be confirmed visually by observing the longitudinal section of the flap at vertical incision. • The incision is started by holding the flap straight up with a hemostat. • A pair of scissors (preferably Goldman Fox #1) is then inserted between the periosteum and the underlying soft tissue.
  • 9. • The periosteum is incised with upward motion using the scissors, lifting the layer of periosteum to avoid damage to the vessels and nerves around the surgical field. • After the incision has been made, it is always necessary to go back to the incision line with the blunt side of the scissors to make sure the thin periosteal fibers are not left behind. This confirmation will maximize the release of every incision.
  • 10. • The subsequent incision starts 1mm apical from the first one and the same technique is repeated here. • Once adequate release of the flap is obtained by multiple incisions with the UMST, flaps are gently advanced and sutured with horizontal mattress and interrupted sutures to secure the closure.
  • 11. Clinical Case: • A systemically healthy 42-year-old woman presented with a chief complaint of masticatory disturbance and deviation of the jaw to the left after losing the left mandibular molars. • The bone defect was a combination of horizontal and vertical atrophy with the horizontal being more significant. • The patient elected to have implant treatment and the following treatment plan was proposed and accepted by the patient. • One hour before the surgery, amfenac sodium (25 mg) and bacampicillin hydrochloride (750 mg) were administered. • The patient rinsed her mouth with 0.025% benzalkonium chloride solution prior to the surgery.
  • 12. • After administration of local anesthesia with Xylestesin, incisions including a vertical incision were made and a full-thickness flap was elevated. • The mental foramen was marked and the mental nerve was isolated. • Releasing incisions were made with the UMST. • Following confirmation of adequate flap release, decortication of the surrounding bone was performed. • Three Osseotite NT implants were placed with an insertion torque of 50 Ncm.
  • 13. • While preparing the osteotomy using Quad Shaping Drills, autogenous bone was harvested. • An 18 × 25-mm Titanium Mesh was customized to cover the buccal dehiscence about 8 mm in height with the expectation that the mesh would allow for 3 mm of horizontal bone regeneration. • Harvested autogenous bone was placed to cover the exposed implant surface and then deproteinized bovine bone mineral (Bio-Oss) was used to fill the rest of the space in the mesh. • The mesh was stabilized with customized cover screws to stabilize the bone graft material.
  • 14. • Before the flaps were closed, a collagen membrane (Ossix 25 × 30 mm) was placed over the mesh. • The wound was completely closed with passive flaps achieved by the UMST. • Horizontal mattress and interrupted sutures with 5-0 Monocryl were used to secure the closure of the flaps. • Postoperative antibiotics (Bacampicillin hydrochloride 250 mg every 8 hours for 7 days) and analgesics (amfenac sodium 25 mg, as needed) were given along with postoperative instructions.
  • 15. • At 9 months after the surgery, the second-stage surgery was performed to expose the implants. • Upon removal of the titanium mesh, excellent bone regeneration was noted. • A large defect initially 8 mm in size was completely covered with 3 mm of horizontal bone. • A free gingival graft was performed to augment the zone of keratinized tissue around the implants.*
  • 16. • After the healing of the soft tissues, the final restoration was constructed and a screw-retained implant- supported porcelain-fused-to-zirconia bridge replacing the mandibular left first and second premolars and first molar was delivered to the patient • The 3-year postoperative radiograph demonstrated the stability of the crestal bone.
  • 17. DISCUSSION • In this report, the authors introduced the UMST for flap advancement and presented an anatomically challenging case that involved the use of UMST. • Based on the experiences of more than 100 cases performed over the last 9 years, the authors recommend this surgical technique, particularly in cases where potential damage to nerves or blood vessels are of concern. • Even with an understanding of the critical anatomy, surgical procedures including releasing incision can be challenging when anatomical abnormalities are present. • For releasing incision, a single incision can be made with a scalpel instead of scissors.
  • 18. • However, it can be difficult to control the depth of the incision with a surgical blade. • Different approaches for flap advancement to attain tension-free primary closure have been reported.* • One example is the use of a buccal periosteal pocket technique.* • This technique increases soft tissue mobility and elasticity and allows better support of bone substitute material. • However, it is necessary to advance a periosteal elevator deep into the subperiosteal soft tissue area. • This could still cause injuries to microvasculature and nerves.
  • 19. • With the UMST, multiple shallow incisions are made using curved scissors with the tip carefully controlled and facing upward, avoiding potential damage to underlying tissues. • Even though most incisions made with the scissors are clean and accurate, some thin periosteum fibers can be left behind. • Thus, it is important to go back with the blunt side of the scissors to trace the incision and identify any tags from the remaining fibers. • Once the fibers are identified, the scissors should be used to cut them to make sure the flaps can be fully extended. • Any curved scissors of appropriate size can be applied to perform the UMST.
  • 20. • However, based on treatment experience, a preferred choice is Goldman Fox #1. • Some modifications, such as a slightly longer handle and a duller tip, may greatly improve its handling and safe operation. • An area where it is difficult to place a releasing incision is near the maxillary tuberosity, where the coronoid process is present. • Due to the limited access to the area, it is extremely difficult to control the incision with a surgical blade. • If the incision is too deep in this area, damage to the pterygoid plexus could occur.*
  • 21. • Based on the authors’ experience, the UMST can provide easier access to precisely place 1mm incremental incision lines even in undercut areas of the maxillary tuberosity. • With the UMST, the use of a microscope or surgical scope is recommended to control the angle and depth of the incision and the extension of the flap to further minimize potential complications. • Information is still limited on the effects of different incision placements having on the amount of flap extension.* • Therefore, clinicians have to predict the amount of flap extension based on their experiences or assumptions.* • Further studies are needed to establish safe and predictable releasing incision design for flap management.
  • 22. CONCLUSIONS • Within the limitations of the authors’ experience, the upward- motion scissors technique (UMST) may prove to be an effective method to secure flap advancement in areas where a surgical blade is difficult or unsafe to make releasing incisions. • More studies are needed to validate the clinical results of the technique presented here.
  • 23.
  • 24. REFERENCES • 01. Greenstein G, Greenstein B, Cavallaro J, Elian N, Tarnow D. Flap advancement: Practical techniques to attain tension-free primary closure. J Periodontol 2009;80:4–15. • 2. Machtei EE. The effect of membrane exposure on the outcome of regenerative procedures in humans: A meta- analysis. J Periodontol 2001;72:512–516. • 3. Fugazzotto PA. Maintaining primary closure after guided bone regeneration procedures: Introduction of a new flap design and preliminary results. J Periodontol 2006;77:1452–1457. • 4. Leong DJ, Oh TJ, Benavides E, Al-Hezaimi K, Misch CE, Wang HL. Comparison between sandwich bone augmentation and allogenic block graft for vertical ridge augmentation in the posterior mandible. Implant Dent 2015;24:4–12 • 5. Ten Cate AR. Oral Histology: Development, Structure, and Function, ed 2. St Louis; Mosby, 1985. • 6. Fiorellini JP, Stathopoulou PG. Anatomy of the periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA (eds). Carranza’s Clinical Periodontology, ed 12. St Louis: Elsevier Saunders, 2015:34. • 7. Keller GJ, Cohen DW. India ink perfusions of the vascular plexus of oral tissues. Oral Surg Oral Med Oral Pathol 1955;8: 539–542. • 8. Folke LE, Stallard RE. Periodontal microcirculation as revealed by plastic microspheres. J Periodontal Res 1967;2:53– 63. • 9. Velvert P, Peters CI. Soft tissue management in endodontic surgery. J Endod 2005;31:4–16. • 10. Park JC, Kim CS, Choi SH, Cho KS, Chai JK, Jung UW. Flap extension attained by vertical and periosteal-releasing incisions: A prospective cohort study. Clin Oral Implants Res 2012;23:993–998.
  • 25. REFERENCES • 11. Fugazzotto PA. Maintenance of soft tissue closure following guided bone regeneration: Technical considerations and report of 723 cases. J Periodontol 1999;70: 1085–1097. • 12. Warrer K, Buser D, Lang NP, Karring T. Plaque-induced peri-implantitis in the presence or absence of keratinized mucosa. An experimental study in monkeys. Clin Oral Implants Res 1995;6:131–138. • 13. Schrott AR, Jimenez M, Hwang JW, Fiorellini J, Weber HP. Five-year evaluation of the influence of keratinized mucosa on peri-implant soft-tissue health and stability around implants supporting fullarch mandibular fixed prostheses. Clin Oral Implants Res 2009;20:1170–1177. • 14. Thoma DS, Buranawat B, Hämmerle CH, Held U, Jung RE. Efficacy of soft tissue augmentation around dental implants and in partially edentulous areas: A systematic review. J Clin Periodontol 2014; 41(Suppl 15):S77–S91. • 15. Steigmann M, Salama M, Wang HL. Periosteal pocket flap for horizontal bone regeneration: A case series. Int J Periodontics Restorative Dent 2012;32: 311–320. • 16. Li J, Xu X, Wang J, Jing X, Guo Q, Qiu Y. Endoscopic study for the pterygopalatine fossa anatomy: Via the middle nasal meatus-sphenopalatine foramen approach. J Craniofac Surg 2009;20: 944– 947.

Notes de l'éditeur

  1. 01. Greenstein G, Greenstein B, Cavallaro J, Elian N, Tarnow D. Flap advancement: Practical techniques to attain tension-free primary closure. J Periodontol 2009;80:4–15. 2. Machtei EE. The effect of membrane exposure on the outcome of regenerative procedures in humans: A meta-analysis. J Periodontol 2001;72:512–516. 3. Fugazzotto PA. Maintaining primary closure after guided bone regeneration procedures: Introduction of a new flap design and preliminary results. J Periodontol 2006;77:1452–1457. 4. Leong DJ, Oh TJ, Benavides E, Al-Hezaimi K, Misch CE, Wang HL. Comparison between sandwich bone augmentation and allogenic block graft for vertical ridge augmentation in the posterior mandible. Implant Dent 2015;24:4–12
  2. 5. Ten Cate AR. Oral Histology: Development, Structure, and Function, ed 2. St Louis; Mosby, 1985. 6. Fiorellini JP, Stathopoulou PG. Anatomy of the periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA (eds). Carranza’s Clinical Periodontology, ed 12. St Louis: Elsevier Saunders, 2015:34. 7. Keller GJ, Cohen DW. India ink perfusions of the vascular plexus of oral tissues. Oral Surg Oral Med Oral Pathol 1955;8: 539–542. 8. Folke LE, Stallard RE. Periodontal microcirculation as revealed by plastic microspheres. J Periodontal Res 1967;2:53–63. 9. Velvert P, Peters CI. Soft tissue management in endodontic surgery. J Endod 2005;31:4–16.
  3. 10. Park JC, Kim CS, Choi SH, Cho KS, Chai JK, Jung UW. Flap extension attained by vertical and periosteal-releasing incisions: A prospective cohort study. Clin Oral Implants Res 2012;23:993–998. 11. Fugazzotto PA. Maintenance of soft tissue closure following guided bone regeneration: Technical considerations and report of 723 cases. J Periodontol 1999;70: 1085–1097.
  4. Xylestesin - Lidocaine hydrochloride/Adrenaline (epinephrine) - 20 mg/ml + 12.5 micrograms/ml
  5. 12. Warrer K, Buser D, Lang NP, Karring T. Plaque-induced peri-implantitis in the presence or absence of keratinized mucosa. An experimental study in monkeys. Clin Oral Implants Res 1995;6:131–138. 13. Schrott AR, Jimenez M, Hwang JW, Fiorellini J, Weber HP. Five-year evaluation of the influence of keratinized mucosa on peri-implant soft-tissue health and stability around implants supporting fullarch mandibular fixed prostheses. Clin Oral Implants Res 2009;20:1170–1177. 14. Thoma DS, Buranawat B, Hämmerle CH, Held U, Jung RE. Efficacy of soft tissue augmentation around dental implants and in partially edentulous areas: A systematic review. J Clin Periodontol 2014; 41(Suppl 15):S77–S91.
  6. Buccal periosteal pocket technique - The flap design results in a periosteal pocket, which allows filling of bone-grafting material while facilitating primary tension-free soft tissue closure by splitting of the mucosa. The flap gives stability to the augmented volume within the pocket. 01. Greenstein G, Greenstein B, Cavallaro J, Elian N, Tarnow D. Flap advancement: Practical techniques to attain tension-free primary closure. J Periodontol 2009;80:4–15. 15. Steigmann M, Salama M, Wang HL. Periosteal pocket flap for horizontal bone regeneration: A case series. Int J Periodontics Restorative Dent 2012;32: 311–320. 15. Steigmann M, Salama M, Wang HL. Periosteal pocket flap for horizontal bone regeneration: A case series. Int J Periodontics Restorative Dent 2012;32: 311–320.
  7. 16. Li J, Xu X, Wang J, Jing X, Guo Q, Qiu Y. Endoscopic study for the pterygopalatine fossa anatomy: Via the middle nasal meatus-sphenopalatine foramen approach. J Craniofac Surg 2009;20: 944–947.
  8. 10. Park JC, Kim CS, Choi SH, Cho KS, Chai JK, Jung UW. Flap extension attained by vertical and periosteal-releasing incisions: A prospective cohort study. Clin Oral Implants Res 2012;23:993–998.