1. GEL ELECTROPHORESIS
JESSICA PARTIN

2. WHAT IS GEL ELECTROPHORESIS?
o A method used in biology to separate DNA strands
to create a DNA fingerprint
o Separates the DNA by size lengths
o Uses an electric current to separate DNA
FINAL
PRODUCT 

3. STEP 1
Extract DNA from

organism and place in
tubes

4. STEP 2
Polymerase Chain Reaction
*this is used
to make
millions of
copies of the
DNA
(this is before the process of gel
electrophoresis)

5. PCR STEPS
 Denature= rips apart DNA
 Lasts about 1 minute
 At about 94˚F
 Annealing= starts the coping DNA
 Lasts about 45 seconds
 At about 54˚F
 Extension= finishes copying DNA
 Lasts about 3-5 minutes
 At about 72˚F
 Repeat 30-40 times

6. STEP 3
Place restriction
enzymes in DNA
(This cuts the DNA into different lengths)

7. STEP 4
Dye the DNA and
place it into gel
(the gel is usually agarose gel)

8. STEP 5
Run electrical current
though gel
(negative to positive)

9. STEP 6
Place in stain, then under
UV lighting.
The smallest DNA
fragments move the
fastest and farthest

10. USES
 Evidence in criminal cases
 To determine paternity
 To diagnose genetic diseases
 Help to determine kinship in animals
 Compare similarities and differences
between species

11. PROS VS. CONS
Small amount of Hazardous
starting material material
(DNA) needed
Expensive
DNA can be detected
regardless of size of Very time
consuming
DNA
*The Macgyver Project used gel electrophoresis

12. FUNNIES;)

13. GO TO THIS WEBSITE:
This is a very helpful interactive site that I got
most of my information from. If you would
like to know more about gel electrophoresis
in more detail, I highly suggest this site!
learn.genetics.utah.edu/content/labs/gel