1. Summary:
Outgoing individual with a passion for science and medicine. Has a special interest in neurodegenerative diseases and
neuropharmacology with an expertise in molecular mechanisms of Huntingtons Disease. Two years’ experience working in a
Huntington’s Disease focused research laboratory at Western Washington University preparing tissue and conducting various staining
procedures. I’m ultimately looking to more fully develop my laboratory experience in preparation for graduate school opportunities.
Relevant Experience:
Research Assistant, Western Washington University November 2012 - March 2015
ᵒ Proficient at transcardial perfusions and can prepare formaldehyde fixed and frozen animal tissue and section using a
cryostat. Conduct staining using immunohistochemistry, immunocytochemistry, Oil Red O, Hematoxylin and Eosin Y, and Cresyl
Violet. Ability to delicately mount 25 micron free floating sections onto microscope slides or dry mount 5 micron sections directly
from a cryostat. Competent in quantification and analysis of data using R statistical programming language. Recognize aberrant
neuronal and hepatic anatomy indicative of disease. Through this experience, I have developed an excellent understanding of
appropriate laboratory maintenance, safety precautions, and general conduct.
Education
Whatcom Community College September 2009 - June 2011
• Associates in Arts and Sciences
Western Washington University September 2011 - March 2015
• Bachelor of Science with an emphasis in neuroscience and molecular biology.
• Scored in the 73rd
percentile on the Biochemistry Graduate Record Examination.
Coursea & edX certified online coursework March 2015 - Current
• HarvardX: PH525.5x Case Study: RNA-seq data analysis • HarvardX: PH525.1x Statistics and R for the Life Sciences •
HarvardX: PH525.8x Case study: DNA methylation data analysis • HarvardX: PH525.7x Case Study: ChIP-seq data analysis •
UQx: BIOIMG101x Introduction to Biomedical Imaging • University Of Toronto: Bioinformatics Methods I • University Of Toronto:
Bioinformatics II
Operation Experience:
ᵒ Peristaltic Perfusion Pump
ᵒ Cryostat
ᵒ Micropippeting
ᵒ Staining racks
ᵒ Proficient with the following microscopes (in order of experience): Epi-fluorescent confocal microscopy and the related
Olympus Fluoview software (Olympus IX81), conventional microscopy, electron microscopy (Vega TS 5136MM), and stereology
using Neurolucida Neuron Tracing Software (Olympus BX51).
Previous Projects (Jeff Carroll’s Lab)
My work with Dr. Carroll was primarily focused on immunohistochemical staining of mouse tissue for the purpose of
establishing end-point pathology markers in our mouse model of Huntington’s Disease. I have been introduced to a wealth of
genomic and molecular techniques and received a well-rounded introduction to the laboratory atmosphere. I was fortunate enough to
receive two paid summer internships to continue my work in lab while between academic quarters. In addition, my work was
summarized in poster format at Western’s Scholars Week and Psych Fest where I was awarded Best Poster by the dean of the college.
Andrew Keefe
237 Wells Ave North • Renton, Washington • 98057
(360)599-4705
Aj.keefe007612@Gmail.com

2. References
Contact by email is preferred
Jeff Carroll, Ph.D.
Assistant Professor of Neuroscience
Department of Behavioral Neuroscience
Western Washington University
Academic Instructional Center, Room 592
516 High St
Bellingham, WA 98225
(360) 650 - 2928
Jeff.Carroll@wwu.edu
Relation: Principle Investigator
Mike Mana, Ph.D.
Associate Professor
Department of Behavioral Neuroscience
Western Washington University
Academic Instructional Center, Room 598
516 High St
Bellingham, WA 98225
(360) 650-7311
Mike.Mana@wwu.edu
Relation: Academic advisor at WWU
Blair Duncan, Ph.D
Behavioral Neuroscience Technician
Department of Behavioral Neuroscience
Western Washington University
Academic Instructional center, Room 556
516 High St
Bellingham, WA 98225
(360) 650 - 2194
Blair.Duncan@wwu.edu
Relation: Provided lab equipment training and support
Sydney Coffey, BS
Mouse Research Technologist I
Western Washington University
(720) 938 - 7809
Coffeysydney@gmail.com
Relation: Coworker in Dr. Carroll’s lab