internship

1. SPRING TERM
2015 AMELIA PAYNE’S EXIBITION

2. INTRODUCTION
• Weiss Lab
• Classes
 Visualizing Algebra
 Chemistry
 Italian
• Crash Course World History
• Q & A

3. THIS IS NOT A GATEWAY EXHIBITION

4. WEISS LAB
2015

6. AMAZING PEOPLE
Thank you to all the amazing people who gave me this opportunity, and helped me learn

7. PROJECTS
• HARVESTING MOUSE LUNGS
• PHOTOGRAPHING MICROSCOPY SLIDES
• PH TESTING
• SODIUM DEOXYCHOLATE MYSTERY
• STORAGE SOLUTIONS

8. HARVESTING MOUSE LUNGS
To be used in experiments involving the
decellularization and recellularization of
lungs
Lung
Cannula in
tracia
Lung being ventilated during recelurazation
WHY
Reacherch involving the removal of
cells(decelurazation) and re-growing
cells(recellurazation) may eventually help
us to grow human lungs for transplant

9. PHOTOGRAPHING SLIDES
Of Decellurised and Recellurised lung
and assembling PowerPoints of the slides
Recellurised mouse lung slide
ventilated with 150ml of airThe microscope used to photograph slides
Native pig lung slide

10. PH TESTING
• WHY: Ph. tested washes to insure that the PH would not effect the
decal process unduly
• HOW: a Ph. testing system.
• Small amounts of each weak acids or bases and added to neutralize
the Ph.
• The small amounts( > 35 μl in most cases demonstrated) that the Ph
was close to neutral
• The Ph. was close to neutral and will not impact the decell process

11. DATA
Average Ph. Of washes
Di Triton NaCl DNase
6.11 6.42 4.9 6.28
DI Triton NaCl Dnase
7.35 7.39 7.35 7.79
DI Triton NaCl Dnase
20.5 29.4 25.4 1.25
Average Ph. ReachedAverage NaOH to neutral UL

12. CHEMISTRY CONNECTION
I learned about chemical reactions and
writing formulas in chemistry class
When doing the PH neutralizing
experiment a interesting reaction
occurred. Adding the soluble hydrochloric
acid to the soluble Sodium deoxycholate
solution caused a insoluble participate to
form, I set out to find out why
The Plot Thickens

13. THEORY'S
• I suspected a chemical reaction like the ones I
had been learning about in chemistry class
• I wrote a formula like I had and researched the
solubility of possible products
• All the possible outcomes were soluble but a
precipitate had formed. Why?
The formula
HCl(aq) + C24H39NaO4(aq) → x(aq) + y(s)

14. LIKE ANYONE EVER GOT A GOOD ANSWER ON YAHOO

15. CASE CLOSED
• It was my former mentor Darcy who eventually
solved the riddle
• It wasn't a chemical reaction at all
• Sodium Dexoycolate is soluble in water under a
certain set of conditions
• It is soluble when the PH is above 5
• The added hydrochloric acid locally dropped the
Ph. below 5 causing the Sodium Dexoycolate to
leave solution
• Product information sheets contain useful data,
like solubility conditions.
• Always check the product information sheet, and
the material safety data sheet for any chemical
you are using
HCl(aq) + C24H39NaO4(aq) → HCl(aq) + C24H39NaO4 (s)
The formula

16. STORING LUNGS
We can not store decellurised lungs for
longer then 3 months
Enzymes may digest proteins, making
the lungs unviable for recellurization
Water crystals are sharp and can
damage cells and matrix. Water
also expands and bursts cells
when it freezes.
Cryogenic chemicals that lower
the freezing point are often
dangerous to cells
I researched methods used to
store skin grafts

17. SOLUTIONS
Freezing at a controlled rate in
a controlled rate freezer in a
cryogenic solution is another
In the future I will help to
create a protocol for
preserving the decellurised
lung matrix
Deep freezing in liquid
nitrogen may be a
possible method
propylene glycol a cryogenic solution

18. WHAT I LEARNED
• To work hard
• How to take clear miscopy photos
• How tissue is stored
• How to test for Ph.
• How to interact with coworkers
• How to win sparkle quizzes when
One person cant spell and the
other only knows the answers in
germen

19. Q & A