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Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
1Department of Orthopaedic and Trau...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
stem	
  cell	
  
What	
  is	
  a	
 ...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
1	
  stem	
  cell	
  
Self	
  renew...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Where	
  are	
  stem	
  cells	
  fo...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Tissue stem cells
muscles	
  
skin	...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Principles of renewing tissues
Stem...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Stem	
  cell	
  niches	
  	
  
Dire...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Homeostasis	
  	
  
•  The ability ...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Mesenchymal stem cells (MSCs)
MSC	
...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Friedenstein et al. 1968
A.J. Fried...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Pittinger, Science 1999
hMSC
human ...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Sacchetti, Cell 2007
Self renewal o...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Rom...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
MSCs function for tissue regenerati...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Joint and Cartilage
Large bone gap
...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
The Intervertebral disc!
Interverte...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Intervertebral disc degeneration!
D...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Therapeutic hypothesis
Differentiat...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Preliminary study
Evaluation of the...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Mechanisms of interction ?!
Vadalà ...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Clinical translation
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Rom...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Università Campus Bio-Medico di Rom...
Università Campus Bio-Medico di Roma
Via Álvaro del Portillo, 21 - 00128 Roma - Italia
Grazie!
www.unicampus.it
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Gianluca Vadalà, La bellezza delle cellule staminali (Msc and art)

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Nell’ambito della rassegna culturale di arte, scienza e filosofia “Primavera Americana a Decollatura. Sul filo di ordine a caos” organizzata da Pramantha Arte contemporary art gallery
dal 1 al 17 Maggio prossimi presso l’Hotel Cardel di Decollatura, il 10 Maggio l’associazione AstroPramantha aderisce alla giornata nazionale "Occhi su Saturno" con una video proiezione di immagini dal microcosmo del corpo umano nel lavoro del medico ricercatore Gianluca Vadalà dell’Università Campus Bio-Medico di Roma.

Si ringrazia il dott. Gianluca Vadalà per la gentile concessione.

Publié dans : Formation
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Gianluca Vadalà, La bellezza delle cellule staminali (Msc and art)

  1. 1. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia 1Department of Orthopaedic and Trauma Surgery, Campus University Bio-Medico of Rome, Italy 2Cell Factory, Center of Transfusion Medicine, Cellular Therapy and Cryobiology, Department of Regenerative Medicine, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano, Italy La bellezza delle Cellule Staminali Dr Gianluca Vadalà, MD, PhD DIPARTIMENTO DI ORTOPEDIA E TRAUMATOLOGIA DIRETTORE: PROF. VINCENZO DENARO www.cellfactorymilano.com
  2. 2. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia stem  cell   What  is  a  stem  cell?   stem  cell   SELF-­‐RENEWAL   specialized  cell   e.g.  muscle  cell,  nerve  cell   DIFFERENTIATION     http://www.eurostemcell.org
  3. 3. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia 1  stem  cell   Self  renewal  -­‐  maintains   the  stem  cell  pool     4  specialized  cells   DifferenEaEon  -­‐  replaces  dead  or  damaged   cells  throughout  your  life   Why  self-­‐renew  AND  differen8ate?   1  stem  cell   http://www.eurostemcell.org
  4. 4. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Where  are  stem  cells  found?   embryonic  stem  cells   blastocyst  -­‐  a  very  early   embryo   Essue  stem  cells   fetus,  baby  and  throughout  life   http://www.eurostemcell.org
  5. 5. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Tissue stem cells muscles   skin   surface  of  the  eye    brain   breast   intes;nes  (gut)   bone  marrow   tes;cles   http://www.eurostemcell.org
  6. 6. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Principles of renewing tissues Stem  cell   commiIed  progenitors:   -­‐   “transient  amplifying  cells”   -­‐   mul;potent   -­‐   divide  rapidly   -­‐   no  self-­‐renewal   specialized  cells:   -­‐   work   -­‐   no  division   Tissue stem cells http://www.eurostemcell.org
  7. 7. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Stem  cell  niches     Direct contact Soluble factors Intermediate cell stem  cell   niche   Niche   Microenvironment  around  stem  cells  that  provides   support  and  signals  regula;ng  self-­‐renewal  and   differen;a;on   http://www.eurostemcell.org
  8. 8. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Homeostasis     •  The ability to regulate internal conditions, usually by a system of feedback controls Stabilize health and functioning, regardless of the outside changing conditions. •  One piece of homeostasis is the constant or periodic generation of new cells to replace old, damaged, and dying cells •  Adult stem cells fulfill this role through the process of regeneration
  9. 9. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Mesenchymal stem cells (MSCs) MSC   bone  marrow   commiIed  progenitors   Bone  (osteoblasts)   Car;lage  (chondrocytes)   Fat  (adipocytes)   specialized  cells   Tissue stem cells http://www.eurostemcell.org
  10. 10. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Friedenstein et al. 1968 A.J. Friedenstein is generally credited with the discovery of MSC. Mesenchymal Stem Cells! ü  Cells present within the hematopoietic microenvironment of bone marrow ü  spindle-shaped cells (fibroblastic- like) after plastic attachment ü  capacity to form colonies (colony forming unit-fibroblast, CFU-F)
  11. 11. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  12. 12. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Pittinger, Science 1999 hMSC human Skin fibroblast Oil red O Immunostaining for Coll II alkaline phosphatase differentiation capacity of MSCs!
  13. 13. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Sacchetti, Cell 2007 Self renewal of MSC! MSCs have been identified as sinusoidal adventitial reticular cells
  14. 14. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Mesenchymal Stem/Stromal Cells (MSC) osteocyte chondrocyte miocyte tenocyte adipocyteStromal cells Growth factor & Cytokine Differentiation Trophic effect Proliferation Anabolism Reparative activity Anti-inflammation Pittenger 1999 Caplan 2006 Tissue regeneration mechanisms!
  15. 15. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia MSCs function for tissue regeneration Microenvironment   Regenerate   Trophic   BioacEve  Factors   Immuno-­‐   suppressive   Reserve  Cells   Turnover  /   Maintainance   MSCs   Caplan & Dennis, J. of Cellular Biochemistry, 1998
  16. 16. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Joint and Cartilage Large bone gap Tendon lesions Spine Regenerative medicine in Orthopaedic
  17. 17. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia The Intervertebral disc! Intervertebral disc Spinal segment Nucleus Pulposus Anulus Fibrosus Spinal cord Nerve rout
  18. 18. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  19. 19. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  20. 20. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  21. 21. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  22. 22. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  23. 23. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  24. 24. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  25. 25. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  26. 26. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Intervertebral disc degeneration! Degenerative cascade
  27. 27. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Therapeutic hypothesis Differentiation H20 H20 H20 H20 H20 Collagen Proteoglycans Stem cells to supplement disc cell population of a degenerated disc by stem cell transplantation
  28. 28. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Preliminary study Evaluation of the interaction between Mesenchymal Stromal/Stem Cells from bone marrow and Nucleus Pulposus Cells
  29. 29. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Mechanisms of interction ?! Vadalà et al. Spine 2008 MSC NPC Co-coltura - 2 wks Cell sorting Analysis of gene expression profile Double labeling cell system Alginate beads
  30. 30. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  31. 31. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  32. 32. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  33. 33. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  34. 34. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  35. 35. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia
  36. 36. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Clinical translation
  37. 37. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Università Campus Bio-Medico Unità di Ortopedia e Traumatologia Vincenzo Denaro, MD Gianluca Vadalà, MD, PhD Maria Musumeci,PhD Fabrizio Russo, MD Antonino Giacalone, MS Caterina Cattani, MS University of Pittsburgh Stem Cell Research Center Johnny Huard, PhD Spine Research Laboratory James D. Kang, MD Nam Vo, PhD Gwen Sowa, MD, PhD Kevin Bell, PhD AO Research Institute Davos Musculoskeletal Regeneration Program Mauro Alini, PhD Marianna Peroglio, PhD Matteo D’Este, PhD Università di Padova Department of Veterinary Surgery Roberto Busetto, DVM Marco Bernardini, DVM Francesca De Strobel, DVM Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico Milano Cell Factory Rosaria Giordano, MD Lorenza Lazzari, PhD Acknowledgments
  38. 38. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Acknowledgments Finanziamento PRIN Stem Cell Research Group Finanziamento Giovani Ricercatori Research Traveling fellowship AOSpine Europe Young Researcher Award
  39. 39. Università Campus Bio-Medico di Roma Via Álvaro del Portillo, 21 - 00128 Roma - Italia Grazie! www.unicampus.it

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