Sanitation Disinfection and fumigation in Hatchery by Dr M. Ashiq

1. Prepared by
Dr Muhammad Ashiq Toor
DVM , B s c (Zoology)
Islamia University of Bahawal Pur (IUB) (2010 – 2015)
+92-344-499-7375
+92-300-364-2402
Sanitation Disinfection and fumigation in Hatchery

3. Sanitation Disinfection and
fumigation in Hatchery

4. Sanitation
The process of keeping places free from
ØDirt
ØInfection
ØDisease
ØRemoving wastes
The hatcher is
the main source
of
contamination
for the entire
hatchery.
Debris, dead chicks, and chick fluff are
ideal replication sites for bacteria and
fungi
Øby cleaning

5. Cleaning and Disinfection
Completely free of Microbs
Impossible
Regular cleaning and
disinfection control microbs
multiplication
Remove organic materials
A good cleaning removes up
to 85% of microbs

6. Cleaning & Disinfection
Good design
for
Cleaning
Disinfection
Effective
Disease
control in
Hatchery

7. Cleaning Sanitation is always 90% Cleaning
Empty refuse
Soak in
detergents
Wash with hot
water
Rinse
Disinfect
Mach C Solutions
Netherland

8. Disinfectants and detergents for
Equipment
Effective disinfectant
property
Safe for Equipment
Compatible with water
ØSanitizer reduces to “safe levels”
ØDisinfectant kills 100% bacteria
selection

9. Walls, Floors, Ceilings,
Windows, Fans, Ducting, and
Machines Remove dust and debris
 Where necessary, scrub walls and
floors
 Switch on setter machine to warm up
 Broad-spectrum disinfectant detergent
solution to the setter machine
 Use pressure washing and leave them
for drying


Cleaning

11. USA

12. Canada

15. Fumigation ( KmNO4 + Formalin) 1:2
Formaldehyde is effective biocide
Reacting with carboxyl, amino,
hydroxyl and sulfhydryl groups of
proteins
Effective at room Temp and RH 70%

16. Procedure
Fumigation ( KmNO4 + Formalin (3x)
• Sett the eggs in
setter trays
Move them to
fumigation room
• KmNO4 60gram
• Formalin 120ml
• That is 3X concentration
Per 100 cubic
feet
• Circulate for 20 minutes
• Expel gas after that by exhaust fan
• At room tem.

17. Fumigation of eggs in setters (1X)

Eggs should be fumigated
within 12 h after setting
Eggs which have been
incubated for 24-96 h
should not be fumigated

19. Fumigation of hatchers (1x)
•Removal of all chicks
•The cleaning and disinfection of the empty
machine.
•The disinfected egg trays are replaced
•The machine is prepared for the next
batch of incubating eggs.
•The doors and vents should be closed
•Fumigation time should be at least three
hours, or preferably overnight
Procedure

21. Neutralisation of formaldehyde gas
Formaldehyde gas may be neutralised in 10-
15 min using ammonium hydroxide at an
amount equal to half the volume of formalin
used

22. Egg Washing
Easier , Faster
Clean residuals on eggs
Avoid contamination to
other eggs by residuals
Some managers prefer washing on fumigation

24. Egg Washing effectively sanitize the
hatching eggs if
Egg Washing
Proper equipment's
Proper temperature of solution
Washing material, detergents

27. Disinfectants and detergents for
egg washing
Quaternary Ammonium
ROCCAL-D®
10% Quaternary
Ammonium
disinfectant
4.2 %
Sodium
Carbonate
0.4 %
EDTA
Egg Washing
Chlorine based disinfectants
widely used for table eggs and
also safe for hatching eggs
Quaternary Ammonium an
excellent sanitizer

28. Hatchery Waste Disposal
Infertile
eggs
Non-
hatched
eggs
Dead
weak or
unsalable
chicks
Egg
shells
These wastes should be properly disposed of, otherwise they can be a source
of disease infection in the hatchery.

29. Evaluating your total Sanitation
program

Hatchability
percentages
Embryo
mortality
rates
Number
(percentage) of
rotten eggs in
setter
First 4 day
post hatching
mortality rates
of chicks
Results from
Bacterial and
Fungal
cultures

30. MICROBIOLOGICAL MONITORING
OF HATCHERIES

Simplest way is to use agar plates.
For Bacterial cultures - put them in zip lock
bags and put them in Setters for 2 days.
For Fungal cultures put them in zip lock bags
and put them out at room temp for 4-5 days.
Procedure in the hatchery
Put them out in different parts of hatchery
building (label them) for 10 minutes.
The test in the hatchery is performed
routinely once a month
Preparation of media
Agar sausages Agar broth

31. Evaluation of results
Setter & Egg Rooms (after cleaning):
Number of colonies per air plate
Bacteria Mold Rating
0-10 0 Clean
11-20 1-3 Slightly contaminated
21-30 4-6 Moderately contaminated
31 or
more
7 or
more
Heavily contaminated
After incubation of samples at
37°C for 16-20 h, the number of
bacterial colonies growing on
each sample
Setter & Egg Rooms (after cleaning):
Number of colonies per air plate
Heavily contaminated7 or
more
31 or
more
Moderately contaminated4-621-30
Slightly contaminated1-311-20
Clean00-10
RatingMoldBacteria