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Ampoules steralization
1.
2.
3.
4.
5. 1-Filling
Solutions sterilized by filtration are to be
filled under aseptic conditions. The
containers and closures must be properly
cleaned, sterilized and made available
for use at this point in the process.
the product at this stage should be
adequately protected so that it does not
pick up any particulate or microbial
contamination from the environment.
6. 2-Sealing :
Sealing of the filled container should be
done as soon as possible to prevent
the contents from being
contaminated. Sealing represents the
final aseptic procedure. Ampoules are
sealed by melting a portion of the
glass neck with a fine jet of flame.
7. 3-Sterilization
Sterilization is process of complete
destruction or removal of all forms of life.
Except in case of thermolabile
substances, parenteral products are
commonly sterilized after filling and
sealing in the final containers and the
process is called terminal sterilization.
8.
9. Most useful sterilization method is
Autoclaving which employs steam
under pressure. It is probably the
most effective method for
sterilization of aqueous liquids or
substances that can be reached or
penetrated by steam.
Most useful sterilization method is
Autoclaving which employs steam
under pressure. It is probably the
most effective method for
sterilization of aqueous liquids or
substances that can be reached or
penetrated by steam.