Practical Approach For Predicting Retention Time Shift Due To Pressure And Temperature Gradient In UPLC

1. A seminar on
Practical Approach For Predicting Retention
Time Shift Due To Pressure And
Temperature Gradient In UPLC
Master Of Pharmacy
In
Quality Assurance Techniques
Presented By
Mr.khandare B.S.
(Roll no.523)
Sinhgad college of pharmacy,vadgaon pune-41

2. 1
3
2
4
5
VERY SMALL PARTICLE size(<2um)
High speed separation
Improved resolution
Short analysis time
Higher sensitivity

3. INCREASE IN PRESSURE
DECREASE IN PARTICLE SIZE
10K -15k
psi
<2um
30mm
UPLC-COLUMN
Short column length

4. High Pressure(10k-15k )psi
Mobile Phase
Stationary Phase
Column

6. INCREASE IN FLOW

8. EXPERINENT
1
• LOW FLOW
RATE.
• LOW
PRESSURE.
• COLUMN
TEMPERATURE IS
OPTIMUM
• FLOW RATE IS
LOW.
• OVEN
TEMPERATURE
IS 200C above
than 1st
experiment
EXPERINENT
2
EXPERINENT
3
• FLOW RATE
IS HIGH
• LOW
TEMPERATURE
GRADIENT
EXPERINENT
4
• FLOW RATE IS
HIGH
• COLUMN
TEMPERATURE
IS
OPTIMUM

9. EXPERINENT
1
• LOW FLOW
RATE.
• LOW PRESSURE.
• COLUMN
TEMPERATURE IS
OPTIMUM
It Is Used To Define
Reference Condition
2 3 4

10. • FLOW RATE IS
LOW.
• OVEN
TEMPERATURE
IS 200C above
than 1st
experiment
EXPERINENT
2
It is used to determine how
retention factor depends on
temperature
1 2 3

11. 1 2 3 4
EXPERINENT
3
• FLOW RATE IS
HIGH
• LOW
TEMPERATURE
GRADIENT
To determine how retention
factor depends on pressure

12. EXPERINENT
4
• FLOW RATE IS
HIGH
• COLUMN
TEMPERATURE
IS
OPTIMUM
It Estimates The Pressure And The
Average Column Temperatuere As A
function Of Flow Rate With The
Column
1 2 3