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John La Salle - Opening Plenary

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Consortium for the Barcode of Life (CBOL)
Consortium for the Barcode of Life (CBOL)

The document summarizes a "Barcode Blitz" project between the CSIRO Ecosystem Sciences and the Biodiversity Institute of Ontario to rapidly barcode Australian Lepidoptera specimens. Over 10 weeks, they processed 28,000 specimens representing 8,000 species, taking leg samples for DNA barcoding and digitizing collection records. This created a comprehensive barcode reference library that has supported research into taxonomy, biodiversity, and biosecurity applications.

FormationTechnologie
1  sur  30
The “Barcode Blitz”: accelerating the targeted capture of barcode data John La Salle, Beth Mantle CSIRO Ecosystem Sciences, Canberra, Australia Jeff Webb, Rodolphe Rougerie, Paul Hebert Biodiversity Institute of Ontario, Guelph Canada
The “Barcode Blitz” - ANIC Lepidoptera ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The “Barcode Blitz” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The “Barcode Blitz” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Assembly line processing
Assembly line processing
Selecting specimens ,[object Object]
Selecting specimens ,[object Object]
Specimens into array boxes ,[object Object],[object Object]
[object Object]
Specimens databased and geocoded
Specimens into processing boxes ,[object Object],[object Object],[object Object]
Every specimen photographed
Leg removed for barcoding
[object Object]
Specimens back to collection ,[object Object]
The “Barcode Blitz” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The “Barcode Blitz” - what did we achieve Despite an average age of 30 years, COI sequences were recovered from more than 95% of the specimens
The “Barcode Blitz” - what did we achieve Provided the first continent-wide data set for a megadiverse insect group in Australia within a short period of time (about 90% of named Lepidoptera) Provides a model for inclusion in other initiatives aimed at the high-throughput accumulation of priority data sets. Establishes the great value of existing natural history collections as a basis for fast tracking the development of comprehensive DNA barcode libraries.
Despite popular opinion …
The “Barcode Blitz” - Australian benefits ,[object Object],[object Object]
The “Barcode Blitz” - results ,[object Object]
The “Barcode Blitz” - results ,[object Object]
The “Barcode Blitz” - Australian benefits ,[object Object],[object Object]
One example of international benefit ,[object Object],[object Object]
The “Barcode Blitz” - Australian benefits ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Rapid Taxonomy of Australian Xylorictidae ,[object Object],[object Object]
Rapid Taxonomy of Australian Xylorictidae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Accelerated Species Discovery ,[object Object],[object Object],[object Object],[object Object],[object Object]
Thank you Funding: Genome Canada CSIRO Transformational Biology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Contact Us Phone: 1300 363 400 or +61 3 9545 2176 Email: enquiries@csiro.au Web: www.csiro.au

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John La Salle - Opening Plenary

  • 1. The “Barcode Blitz”: accelerating the targeted capture of barcode data John La Salle, Beth Mantle CSIRO Ecosystem Sciences, Canberra, Australia Jeff Webb, Rodolphe Rougerie, Paul Hebert Biodiversity Institute of Ontario, Guelph Canada
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  • 18. The “Barcode Blitz” - what did we achieve Despite an average age of 30 years, COI sequences were recovered from more than 95% of the specimens
  • 19. The “Barcode Blitz” - what did we achieve Provided the first continent-wide data set for a megadiverse insect group in Australia within a short period of time (about 90% of named Lepidoptera) Provides a model for inclusion in other initiatives aimed at the high-throughput accumulation of priority data sets. Establishes the great value of existing natural history collections as a basis for fast tracking the development of comprehensive DNA barcode libraries.
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