Presentación llevada a cabo por Víctor Flors Herrero de la Universitat Jaume I en el FORO INIA TEMÁTICO DE COLABORACIÓN PÚBLICO-PRIVADA No 18: LA SECA celebrado el 3 de Julio de 2014 en la sede de CICYTEX en Mérida.

1. ASSOCIATED UNIT EEZ(CSIC)-UJIASSOCIATED UNIT EEZ(CSIC)-UJI
RESEARHRESEARH
http://nitropriming.wordpress.com/http://nitropriming.wordpress.com/
Metabolic integration and Cell Signaling Group.Metabolic integration and Cell Signaling Group.
Departamento de Ciencias AgrariasDepartamento de Ciencias Agrarias
y del Medio Natural (CAMN).y del Medio Natural (CAMN).
Universitat Jaume IUniversitat Jaume I

2. Research Lines:Research Lines:

Induced resistance against pathogens

Development of resistance inducers and priming agents. Chromatography
applied to plant defense.

Nitrogen transport

Role of systemin in the establishment of mycorrhizas and induction of systemic
resistance in tomato

Study of Plant-arthropod interactions

Reactive oxygen species and hormonal metabolism in defense priming

3. Balances metabólicos yBalances metabólicos y
hormonales implicados enhormonales implicados en
la resistencia inducidala resistencia inducida
frente a patógenosfrente a patógenos

4. ACCUMULATION
OF TF->FASTER
GENE
TRANSCRIPTION
OR PR
ACCUMULATION
EPIGENETIC
CHANGES
HISTONE
ACETYLATION or
DNA
METHYLATION
PHOSPHORILATION
CONTROL
MAPK CASCADES
2ND BIOTIC STRESS STRONG DEFENCESTRONG DEFENCE
STRESS MEMORY
BASAL DEFENCEBASAL DEFENCE
(WEAK AND SLOW)(WEAK AND SLOW)
DISEASED
PLANT
DISEASED
PLANT
HEALTHYPLANT
HEALTHYPLANT
STRESS
PRE-EXPOSURE
PRIMING AGENT
METABOLITEMETABOLITE
CONJUGATIONCONJUGATION
HORMONALHORMONAL
REGULATIONREGULATION
ROSROS
homeostasishomeostasis

5. 5
Search of hormone relatives using PRECURSOR IONSearch of hormone relatives using PRECURSOR ION
SCANNING and targeted MRMSCANNING and targeted MRM
PIS-PIS-TargetedMetabolomics-UntargetedmetabolomicsTargetedMetabolomics-Untargetedmetabolomics Desarrollo de técnicas cromatográficasDesarrollo de técnicas cromatográficas
para el estudio de la resistencia inducidapara el estudio de la resistencia inducida

6. 3 different priming stimuli
CommonCommon
metabolomicmetabolomic
fingerprint offingerprint of
primingpriming
Non targeted Metabolomic ApproachesNon targeted Metabolomic Approaches

7. PROPUESTA DE COLABORACIÓNPROPUESTA DE COLABORACIÓN
INVESTIGACIÓN A CORTO PLAZO EN FUNCIÓN DEL
CONOCIMIENTO DE PARTIDA
POOL OF
METABOLITE WITH
PRIMING OR INDUCED
RESISTANCE POTENTIAL
Stimuli: resistant genotypes from the germplasm bank; treatments; other related
plant species with enhanced resistance
Stimulus 1
Stimulus 2
Stimulus 3

8. Current Group Members

Dr Víctor Flors; TU

Dr Miguel Cerezo; TU

Dra Paloma Sánchez Bel, RyC

Jordi Gamir, PhD student

Blas Agut, PhD student
http://nitropriming.wordpress.com/http://nitropriming.wordpress.com/
New coming members:
Dra. Victoria Pastor

10. Plants can increase their level of resistance is by intensifying the responsiveness of their
immune system upon recognition of selected signals from their environment. This so-
called priming of defence can provide a memory of resistance, which is based on a faster
and/or stronger defence reaction upon pathogen or pest attack.

11. Priming can target various layers of induced defence.
Priming ranges from early responses controlled by adjustments in
hormone-dependent signalling pathways and ROS to long lasting events
Pastor, V., Luna, E., Mauch-Mani, B., Ton, J., Flors, V. Primed plants do not forget (2012)
Environmental and Experimental Botany.

12. SA conjugates
Indolic metabolites