Biology I lab about cellular respiration

1. All organisms require energy
• Energy molecule of the cell is ATP
– Nucleic acid
Energy released
ATP ADP

2. Two main energy releasing
pathwaysAerobic
• Requires oxygen
• Occurs in cytoplasm and
mitochondria
• Creates 36 ATP
Anaerobic
• Doesn’t require oxygen
• Uses another molecule like
nitrate or sulfate
• Occurs completely in
cytoplasm
• Fermentation is an
example
• Two types

3. Aerobic Respiration: An Overview
C6H1206 + 6O2 6CO2 + 6H20 + 36 ATP
1. Glycolysis
• Enzymes break glucose into two 3 carbon pyruvate
molecules
1. Krebs Cycle
• Enzymes break pyruvate into CO2
• Electrons and H+
released
• Electron Transport Chain
• Many ATP made because of H+
and electron gradient
• Oxygen joins with H+
forming the product water

4. 2
2
32

5. Anaerobic Respiration:
Fermentation
• Does not require oxygen
• Does not require a mitochondria
• Only produces 2 ATP
• 2 options

6. 1. Lactic Acid Fermentation
– Used by bacteria and human muscle
1. Alcoholic Fermentation
– Used by yeasts

7. Today’s Experiment
• Track aerobic respiration in insects
• What are some reasons why insects require
energy?
• Compare bean beetles to pill bugs

8. Carbon dioxide Sensor
• What do you expect to observe with carbon
dioxide levels in the insects?
• Will one insect have higher aerobic
respiration than another insect?
• Although we are not measuring oxygen
what is happening to oxygen levels?
• Which insect do you think will undergo
more respiration?