THE USE OF CELLULAR TECHNOLOGY IN TREATMENT OF CHILDREN WITH DEEP BURNS Budkevich Ludmila1, Koroleva Tatiana1 1Moscow Scientific Institute of Pediatrics and Children Surgery (Moscow, Russia).

1. Moscow Scientific Institute of Pediatrics and Children
Surgery
Speransky Children Hospital, Moscow, Russia
THE USE OF CELLULAR
TECHNOLOGY IN TREATMENT
OF CHILDREN WITH DEEP
BURNS
Ludmila Budkevich, Tatyana Koroleva

2. Cellular technologies (grown autologous
keratinocytes, allofibroblasts)
 Difficulties with cultivation of cell layers
 High percentage of complications
 Shortcoming of legal using in Russia

3. ReCell®
Device for receipt autologous cells:
 Keratinocytes
 Melanocytes
 Fibroblasts
 Langerhans cells
Suspension time preparation : 20-30 minutes
Area of coverage max 320 cm2
Biopsy size : 1-4 cm2

4. 2011-2012 years
10 children with deep dermal burns with hot liquid
Age from 8 months to 3 years
From 3 to 25% TBSA
Sex
Boys
Girls
6
4

5. Location of deep dermal burns:
Chest 4
Stomach 4
Upper extremities 6
Lower extremities 2
TBSA of deep dermal
burns:
1-10% (78 – 320 cm2)

6. Performed surgical operation:
Tangential necrectomy
+
Skin grafting of a 1:4 meshed
+
Transplantation of autocells with «ReCell®»
technology

7. Results:
 Mean time of full epithelization of burn wounds
9 day after operation
 Total hospital bed-days: 15 days
 No local infectious complications
 On follow-up - minimal scars
 Pigmentation of scars was close to healthy skin

8. A child aged 1-year old with
deep dermal burn 10% TBSA
7 days after
operation
6 months
after
operation
A burn wound Transplantation
autocells

9. A child aged 1-year old with deep
dermal burn 10% TBSA
A burn wound Transplantation
autocells
11 days after
operation
8 months
after
operation