it is the response of cell for continuous exposure that can be transplanted into the patients pancre purified islets beta cells transplanted through a brain) discrete populations of adult stem cells ge connective tissue) unipotent stem cell able to if the cell is able to form all cell types of the muscle cartilage foetus or any other source culturing of harvested injury or disease placental cord baby teeth diabet fumazone cell damage viability assay tetrazolium compound mitochondrial damage cell proliferation mtt cell proliferation cytotoxic mtt assay assay cellular necrosis assay cytotoxicity assay cytotoxicity mtt mitochondrial damage asssay apoptosis microtiter plate necrosis dili hepatotoxicity drug induced liver damage drug induced hepatotoxic receptor receptor downregulation receptor desenset immunofluorescence phosphorimaging autoradiography non-lysosomal degradation occur via proteosome (ub techniques for determination of downregulation mechanism of receptor down regulation immunofluorescence is the labeling of antibodies o down-regulation drug or toxins ligand may be agonist or antagonist indirect immunofluorescence we need antibody against particular receptor (prot and recycling most membrane receptors are internal up-regulation hormone lysosomal degradation occur via lysosomes neurotransmitter direct immunofluorescence new synthesis receptor down regulation regulation of receptor assessment of chemicals- harmonized activities con raise living standards provides the definite parameters of evaluation pr regulatory agencies guidelines are the consensus document accepted by and high quality medicines are developed and regis effective maintain financial stability and contribute to gro prevent duplication of clinical trials in humans ich oecd fda who industry and independent laboratories to assess sa supports economic growth efficacy and quality of medicines minimize the us need of guidelines when taken in overdoses & sometimes even when intr fever usa: the mcgraw-hills lemasters jj (2002). toxicological sciences : an o gores gj mohali current diagnosis and treatment in gastroenterolog drug) synthesis of plasma proteins (albumin punjab-160062 2. physiological function of liver 2 eosinophilia examples acetaminophen isoniazid 5rot ganey pe (2010). the journal of pharmacology and e mcquaid k.r. hinson ja drug induced hepatotoxicity and its regulatory imp s.l. s.a.s. nagar
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