1. 1
Dr. Colin John Ingham
Born May 24th
1963
Nationality UK
Address Verenigingstraat 36, 3515GE Utrecht , Netherlands
Emails colinutrecht@gmail.com
Contact: via eMail or Linked In
Employment History
Apr 2008 – CSO MicroDish BV Industry www.microdish.nl
Cofounder, investor, shareholder in biotechnology SME dedicated to rapid cell culture.
Member of management team. Responsible for scientific team, publications and projects, IP
and funding (below). Support business case and sales. Investment secured >0.5 M Euro.
First authorship paper in PNAS reported in New York Times (28th
Nov 2011).
Aug 2004 - Mar 2008 Senior Scientist: 70% TI Food & Nutrition (TIFN)
10% Wageningen University, 20% Hospital JBZ
Industry Academic
Development of “million well” culture chip and that lead to the creation of MicroDish BV.
 Winner “Zilveren Zandloper” Biannual biotechnology innovation award (2008).
 Semi Finalist Prize Winner: New Ventures Business Competition (2008).
 Winner TIFN publication prize (2008).
Feb 2002 - July 2004 Senior Scientist, PamGene BV, (NL/DK) Industry
Senior technical role in SME Biotechnology Company. Creator cellular arrays.
Jan 1999 - Jan 2002 Senior Scientist, Biogeny plc. (UK) Industry
Biotech company spun out of Imperial College London. Development of therapeutic protein
delivery and allergy diagnostics methods.
Oct 1995 - Dec 1998 HEFC Research Fellow, Nottingham Uni. (UK)
Primary academic investigator – molecular biology of transcription.
Mar 1992 - Sept 1995 Postdoc. Genetics, Glasgow University. (UK)
Jan 1992 - Feb 1992 Consultant to Cyanamid (UK) Industry
Jan 1990 - Dec 1991 Postdoc. University of Stirling (UK)
Jan 1988 - Dec 1989 University Wisconsin-Madison (USA): Damon
Runyon Research Fellow.

2. 2
Education
Oct 1982-Jun 1984 B.Sc. Microbiology – 1st
class honours
University College London; Botany & Microbiology
Oct 1984-Nov 1987 Ph.D. Microbiology - UCL/Oxford University.
Thesis: "Mechanism of Chemotaxis in R. sphaeroides"
Post University Courses
 Cold Spring Harbor Advanced Molecular Genetics, USA (1989)
 Cold Spring Harbor Protein Purification, USA (1997)
 EMBO Advanced Molecular Medicine, Sweden (2000)
 Imperial College London: Scientific Management Course, UK (2000)
 Imperial College London: Basic C++ Programming, UK (2001)
 BBSERC: Perl for Bioinformatics, UK (2002)
 EMBL Bioinformatics Course, UK (2003)
 Biobusiness Masterclass, NL (2005)
 Bioinformatics for Marine Microbiology, DE (2013)
Bioinformatics and IT Skills
 Familiar with Windows/Mac OS X Specialised molecular biology, bioinformatics and cellular software
and servers. Basic scripting. Office. Vassar Statistics.
 Quantitative (ImageJ) and illustrative (PhotoShop, Corel Draw) graphics software.
 Maintain MicroDish web site: www.microdish.nl (2008-2011).
Funding Obtained
 2016 MarPipe Marie Curie ITN (PhD student)
 2015 Friesland Fermentalg Manufacturing Grant (75K)
 2013 NanonextNL Valorization Grant (€ 200K with CCM and KIT, 100% subsidy)
 2012-2015 MaCUMBA (EU FP7 € 490K + 50K extension, 75% subsidy)
 2011-2013 Surfixit (270K 65% subsidy)
 2011-2014 Evotar (EU FP7 € 300K, 80% subsidy)
 2011-2015 MicroNed Pathogen Detection (225K, 60% subsidy)
 2011-2015 HotZyme High Throughput Screening (EU FP7 € 450K, 80% subsidy)
 2010-2013 SaWa Water Sensor Consortium (€ 214 50% subsidy)
 2010-2015 BE-Basic Consortium (€ 375K 50% subsidy)
 2008-2011 NanoNed with KIT and CCM (€ 25K)
 2010-2011 RAAK with ErasmusMC/Hogeschool Leiden and River Diagnostics (€ 88K)
 2010-2014 Biomonar (EU Biosensor Consortium € 106K, 80% subsidy)
 2008-2011 Nano4Vitality (€ 175K, 60% subsidy)
 2007-2008 Food and Nutrition Delta (€ 29K)
 2007-2008 NWO Valorization grant (€ 25K)
 2006 North Brabants Life Science (€ 40K), TIFN Lead Proposal (€ 160K)
 1994 University start-up funds (£ 50K)
 1993-1996 Wellcome Trust Grant (£ 110K)
 1990-1992 MRC Grants (£ 225K)
 1988-1990 Damon Runyon Fellowship (Fellowship for US)
Other Activities
o Involvement in the digital arts (Photoshop CS, microbial movies, macro photography, scientific
movies):
o Editor of papers for non-native English speakers.
o Steering and Exploitation Committee FP7 Project: MaCUMBA.
o Outdoors and active pursuits. 151 peaks above 3,000 feet climbed/walked in Scotland, Outward
Bound Winter Mountain Craft course completed (1991). Mountain walking (North America, Spain,
UK, South America).
o Running.
o Member American Society Microbiology (2014 0nwards)

3. 3
Appendix I – Publications
1. Armitage JP, Ingham CJ and Evans MCW (1985) The interaction of aerotaxis and phototaxis in Rhodobacter sphaeroides J Bacteriol 161:
967-972.
2. Ingham CJ and Armitage JP (1987) Chemotaxis in Rhodobacter sphaeroides J. Bacteriol 169: 5801-5807
3. Ingham CJ, Buechner MB and Adler J (1990) Effect of outer membrane permeability on chemotaxis in Escherichia coli J Bacteriol 172: 3577-
3583.
4. Smith MCM, Ingham CJ, Owen CE and Wood N (1992) Gene expression in the Streptomyces temperate phage øC31 Gene 115: 43-48.
5. Ingham CJ and Smith, MCM (1992) An early transcription map of bacteriophage øC31 Gene 122: 77-84
6. Ingham, CJ, Crombie, HJ, Bruton, CJ Chater, KF, Hartley, NM, Murphy, GJP, and Smith, MCM (1993) Multiple novel promoters from the
early region of the Streptomyces temperate phage øC31 are activated during lytic development Mol Microbiol: 9: 1267-1274.
7. Ingham, CJ, Owen, CE, Wilson, SE, Hunter, IS, Smith, MCM (1994) An operator associated with autoregulation of the repressor gene in
actinophage øC31 is found in highly conserved sites in intergenic regions in the phage genome Nucleic Acids Research 22: 821-827.
8. Ingham, CJ, Hunter IS and Smith MCM (1995) Rho-independent terminators without 3’ poly-U tails from the early region of actinophage
øC31 Nucleic Acids Research 23: 370-376.
9. Wilson, SE, Ingham, CJ Hunter IS, and Smith MCM (1995) Control of lytic development in the Streptomyces temperate phage øC31 Mol
Microbiol 16: 131-143.
10. Ingham, CJ, Hunter, IS and Smith, MCM (1996) Isolation and sequencing of the rho gene from Streptomyces lividans ZX7 and
characterisation of the RNA-dependent ATPase activity of the over-expressed protein J Biol Chem 271, 21803-21807.
11. Ingham, CJ, Dennis, J and Furneaux, PA (1999) Autogenous regulation of Rho expression and the requirement for Nus factors in Bacillus
subtilis Mol Microbiol 31: 651-663.
12. Ingham, CJ (1999) Characterisation of the enzymatic and RNA-binding properties of the R sphaeroides 241 Rho homologue Biochem
Biophys Acta 1446: 115-125.
13. Ingham, CJ and Furneaux, PA (2000) Mutations in the -subunit of the B subtilis RNA polymerase that confer both rifampicin resistance and
hypersensitivity to NusG Microbiology 146:3041-3049.
14. Bacarese-Hamilton T, Mezzasoma, L, Ingham CJ, Ardizoni A, Rossi, R and Crisanti A
(2002) Detection of allergen-specific IgE on microarrays by use of signal amplification techniques Clinical Chemistry 48:1367-1370.
15. Wu, Y, Ingham CJ, et al (2004) Quantitative assessment of a novel flow-through porous microarray for the rapid analysis of gene
expression profiles Nucleic Acids Acid Res 34.
16. Ingham, CJ, van den Ende M, Pijnenburg, PC, Wever, PC and Schneeberger, PM (2005) Growth of microorganisms in a multiplexed format
on a highly porous inorganic membrane (Anopore) App Environ Micro 71:8978-8981.
17. Ingham, CJ, van den Ende M, Wever, PC and Schneeberger, PM (2006) Rapid antibiotic sensitivity testing and trimethoprim-mediated
filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support Journal Medical Microbiology 55:1511-
1519. Cover illustration.
18. Ingham CJ, Sprenkels, A, Bomer JG, Molenaar
,
D, van den Berg, A, van Hycklama Vlieg, JET and de Vos, WM (2007) The million well Petri
Dish: highly subdivided microbial growth chip constructed on a porous ceramic support. PNAS 46:18217-18222. Cover illustration. Top 2
download for publication month Nov 2007.
19. den Besten, HMW, Ingham, CJ, van Hylckama Vlieg JET, Beerthuyzen, M Zwietering, MH, and Abee, T (2007) Use of a direct imaging
method to quantitatively assess the effects of mild and severe salt stress on population heterogeneity of Bacillus cereus ATCC 14579 App
Environ Micro 73:4797-4804.
20. Ingham CJ, and Ben Jacob E (2008) A microscopy study of the role of swarming in pattern formation by Paenibacillus vortex BMC
Microbiology 8:36. Highly Accessed.
21. Ingham CJ, Ben Ayad A, Nolsen K and Mulder, B. (2008) Growth and drug sensitivity testing of Mycobacterium tuberculosis complex on a
novel porous ceramic. International Journal of Tuberculosis Lung Disease 12: 645-650.
22. Ingham, CJ, Beerthuyzen, M and Hycklama Vlieg, JET (2008). Heterogeneity of the stress response of Lactobacillus plantarum WCFS1 to
rapid acidification and during recovery. Appl. Environ. Microbiol. 24:7750-7758. Cover illustration.
23. Ingham CJ and Hycklama Vlieg JET (2008) The MEMS Petri Dish. Lab on a Chip. 8:1604-1617. Cover illustration. Top 2 download for
publication month Sept 2008. F1000 factor 11.
24. Fujii, T, Ingham, CJ et al (2008) Two homologous agr-like quorum sensing systems cooperatively control adherence, cell morphology
and cell viability properties in Lactobacillus plantarum WCFS1. J Bacteriol. 190: 7655-7665.
25. Ingham, CJ, Budding D et al. (2009) The Dienes phenomenon: competition and territoriality in swarming Proteus mirabilis J. Bacteriol.
191:3892-3900. [Joint first authorship]. Cover illustration. F1000 factor 10.
26. Ingham CJ, Sprenkels A, Bomer JG, van den Berg, A, and van Hycklama Vlieg, JET. (2010) High-resolution contact printing and transfer
of massive arrays of microorganisms on porous aluminium oxide. Lab on a Chip 10:1410-1416. F1000 factor 6.
27. Sirota-Madi
,
A, Olender, T, Helman, Y, Ingham CJ et al. (2010) Complete genome of the Paenibacillus vortex reveals grounds for thriving
in complex environments and for advanced social behaviour. BMC Genomics 11:710. Highly Accessed.
28. den Hertog, A., Visser FW, Ingham CJ, FHAG Fey, Klatser P, Anthony RM (2010) Simplified automated image analysis for detection and
phenotyping of Mycobacterium tuberculosis on porous supports by monitoring growing microcolonies. PLoS ONE 5(6).
29. Sieuwerts S. Ingham CJ et al. (2010) Mixed culture transcriptome analysis reveals molecular basis and consequences of co-culture
growth in Streptococcus thermophilus and Lactobacillus bulgaricus. Appl. Environ. Microbiol. 76:7775-84.
31. ter Maat J, Regeling R, Ingham CJ, Weijers CAGM, Giesbers M, de Vos WM, Zuilhof H. (2011) Organic modification of porous anodic
alumina with terminal alkynes and its biofunctionalization. Langmuir 27:13606-17.
32. Ingham CJ, ter Maat J, de Vos WM. (2011) Where bio meets nano: the many uses for nanoporous aluminium oxide in biotechnology.
Biotech Advances: doi:10.1016/j.biotechadv.2011.08.005.
34. Ingham CJ, Kalisman O, Finkelshtein A, Ben Jacob E. (2011) Mutually facilitated dispersal between by swarming Paenibacillus vortex
bacteria and the non-motile fungus Aspergillus fumigatus. Proc Natl Acad Sci 108:19731-6. Reported in NY Times.
34. Ingham CJ, Boonstra S, de Lange M and Schneeberger, P. (2012) Rapid antifungal testing of pathogenic yeast on a porous support.
PLoS ONE 7(3):e33813.
35. Ingham CJ and Schneeberger PM. (2012) Microcolony imaging of Aspergillus fumigatus treated with echinocandins. PLoS ONE e35478.
36. Hesselman MC, Ingham CJ et al. A multi-platform flow device for microbial (co-)cultivation and microscopic analysis. PLoS ONE 7(5):
e36982.
37. Ariel G, Shklash A, Finkelshtein A, Kalisman O, Ingham CJ, Ben Jacob EBJ (2012) Collective navigation of cargo carrying swarms.
Interface Focus. 2:786-798
38. Roth D, Finkelshtein A, Ingham CJ, Helman Y, Sirota-Madi A, Brodsky L, Ben-Jacob E (2013) Identification and characterization of
"explorers", a highly motile, antibiotic refractory subpopulation involved in the expansion of swarming colonies of Paenibacillus vortex.
Environ Microbiol doi: 10.1111/1462-2920.12160. Cover illustration.
39. Ariel G, Shklash A, Kalisman O, Ingham CJ, Ben Jacob EBJ (2013) From organized internal traffic to collective navigation of bacterial
swarms. New J Physics. 15: 125019-125037.
40. Yagur-Kroll S., Schreuder, E., Ingham, CJ, Heideman R, Rosen R., Belkin S. (2014). A miniature porous aluminum oxide-based
flow-cell for online water quality monitoring using bacterial sensor cells. Biosens. Bioelec. 64:625-632.
41. Finkelstein A, Roth, D, Ben Jacob E, Ingham CJ (2015) The swarming bacterium Paenibacillus vortex can disperse in toxic terrain by
carrying antibiotic degrading bacteria. mBio. 10.1128/mBio.00074-15.
42. Sayer, C., Szabo, Z., Isupov, M.N., Ingham, C. and Littlechild, J.A., (2015). The structure of a Novel Thermophilic Esterase from
the Planctomycetes Species, Thermogutta terrifontis reveals an open active site due to a minimal ‘Cap’Domain. Frontiers in
Microbiology, 6.
43. Ben-Jacob, E., Finkelshtein, A., Ariel, G., & Ingham CJ. (2016). Multispecies swarms of social microorganisms as moving
ecosystems. Trends in Microbiology. doi: 10.1016/j.tim.2015.12.008.

4. 4
44. Catón L, Yurkov A, Giesbers M, Dijksterhuis J, Ingham CJ. (2016) Physically triggered morphology changes in a novel
Acremonium isolate by culture within precisely engineered microfabricated environments. Environ. Microbiol. Submitted.
45. Polikovsky M, Ingham CJ, Ben-Jacob E, Finkelshtein, A. (2016)Cooperation by day, predation by night – the light sensitive
relationship between the swarming bacterium Paenibacillus vortex and the algae Chlorella vulgaris. Nature. Microbiol. Submitted.
46. Ingham et al A coordinated genetics and optical analysis of structural colour in the novel Flavobacterium IR1. In preparation.
Book Chapters
1. Ingham CJ and Schneeberger P. Microporous and nanoporous materials in microbial culture. Chapter for eBook "The Role of New
Technologies in Microbiological Research and Diagnostics" published by Bentham Books in 2012.
2. Ingham CJ and W.M. de Vos. Moore’s Law and Microbiology. (Dutch) Chapter for book: Microcanon. Published Veen-Magazines in 2011.
3. Ingham CJ. (2012) Dispersal of cargo microorganisms by swarming bacteria. Forum on microbial threats: The Social Biology of Microbial
Communities. Institute of Medicine. National Acadamy Sciences, USA.
4. Finkelshtein, A., A. Sirota-Madi, D. Roth, C.J. Ingham, E. Ben-Jacob (2014). Paenibacillus vortex guide to the wisdom of the crowd. In:
Bio-Communication. Ed.: R. Gordon & J. Seckbach. Chicago, University of Chicago Press: in press.
5. L’Haridon, S. Markx, GH, Ingham CJ, Duthoit, F. Le Blay G. (2016) New approaches for bringing the uncultured into cultured.
Stahl, L and S. Cretuio (eds). The Marine Microbiome – and untold resource of biodiversity and biotechnological potential. Springer Verlag (in
press)
Appendix II – Intellectual Property (Inventor)
1. Ingham, CJ and Anthony, RM Method and device for integrated nucleic acid integrity assessment and analysis. Owner
PamGene BV. WO2004079005. Granted 2006.
2. Hilhorst, R.M, Ingham, CJ, van Damme, HS, Blok, H. Method for high throughput cell-based assays using versatile living
microarrays. WO03102578. Owner PamGene BV. Granted 2006.
3. Ingham, CJ, Hilhorst, RM, van Damme, HS, Blok, H. Novel methods and apparatus for cell-based microarray assays.
WO2005052582. PamGene BV.
4. Ingham, CJ Device for sensing of motile living organisms and uses thereof. WO2005103691. PamGene BV.
5. Ingham, CJ, Masked solid supports and uses thereof. WO2005103699. PamGene BV. Granted 2008.
6. Ingham, CJ, and van Alewijk, D. Cell permeability assay in a living array of multiple cell types and multiple layers of a porous
substrate. WO2005108983. PamGene B.V. Granted 2009.
7. Ingham, CJ, Hylckama Vlieg, J and de Vos W.M. High throughput screening method for assessing heterogeneity of
microorganisms. EPO nr 05103080.7. 2005. Owner WU and DSM.
8. Bomers., J, Sprenkels, A, Ingham, CJ, van Hylckama Vlieg, JET, de Vos, WM, van den Berg, A. Dry etching for
development of grids on Anapore. Owner: DSM. 2005.
9. Ingham, CJ, Hannick, J, van Damme, HS, de Wijn, R and van Beuningen, R. 2005. Masked solid porous supports
allowing fast and easy reagent exchange to accelerate electrode-based microarrays WO2006037527.
10. Ingham, CJ, Bomers, J, Sprenkels, A, van Hylckama Vlieg, JET, de Vos, WM, van den Berg, A. 2007. Method of taking a
plurality of samples. P6016148EP. Owner DSM/Nizo/WUR/Purac.
11. Den Hertog, A, Ingham, CJ, ter Maat J, Anthony, R. 2011. Method for detecting bacteria. Owner MicroDish BV/KIT
Biomedical Research.
12. Ingham, CJ, Hessing M. 2012. Device for in situ cultivation of bacteria. Owner MicroDish BV.
13. Ingham CJ, 2012. Method for Microbial Growth Detection. Dutch filing. Owner MicroDish BV.
Appendix III – Media/Graphic Images
2005: Cover JBZ Hospital yearbook.
2007: Cura magazine (Dutch Hospital Journal): “The New Petri Dish”
2008: Press releases and interest in PNAS article culminating in scientific press attention and commentary articles in
Trends in Biotechnology, Lab-on-a-chip. Articles in RESOURCE, C2W, Assay trade/university journals. C2W article
Zilveren Zandloper. Press Release: New Ventures Final and NRC Next (Newspaper). Article WUR Alumni Magazine.
Article SenterNovem Innovation “InnovatieKrant”. Covers NVVM (Dutch Microbiology Society) yearly report and Kluyver
Centre yearbook 2008.
2009: Contributor to refereed BMC Microbiology Image Library. Cover JBZ Hospital yearbook. Mentioned in article on
Nanotechnology in Sp!ts (Dutch popular newspaper) Sept 11
th
.
2010: Interview in MicroMegazine (Dutch).
2011: Contributor to TNT (post office) booklet showcasing Dutch stamps related to microbiology. Article in New York
Times on PNAS paper (28
th
November 2011). Discussed in Podcast “This Week in Microbiology 23”
2012: Image design (Synthetic Biology) used by science section of the Volkskrant (Dutch national newspaper) Nov.
Medical research Council (UK) Image of the day (Sept 3
rd
)
2015: Article “The Scientist” Show Me Your Moves. Marissa Fessenden. 1 May.
2016: Supporting slideshow for Trends in Microbiology article.
Appendix IV – Teaching
Practical Course: MaCuMBA SUMMER SCHOOL Sampling, Isolation & Cultivation of Marine Microorganisms 12 – 24 July
2015 Texel, the Netherlands.
12 PhD/MSc/Undergraduate student projects (2006-2016)