Research

1. Kingdom Fungi
Introduction
By Dessalegn A

2. Objectives
 Introduction of fungi
 Biology of fungi
 Cell structure, growth and development
 Physiology of fungi
 Nutrition, Temp, UV light, and water
 Classification of fungi

5. Some terms
 mycology
 study of fungi
 mycologists
 scientists who study fungi
 mycotoxicology
 study of fungal toxins and their effects
 mycoses
 diseases caused by fungi

6. •100,000 species
•Only 100 human pathogens, fungi associated
diseases are rising, due to nosocomial
infections and in immunocompromised patients
(ie. HIV, diabetes, transplant folks)
•Aspergillosis, Blastomycosis—pulmonary
infections and dissemination may be involved.
•5,000 plant pathogens=$1 billion/yr
FUNGI

7. Fungal Characteristics
 Eukaryotic
 Most are Multicellular and some are unicellular.
 Heterotrophic
 Absorb nutrients - may be saprobes (absorb from dead
material), parasites, or mutualistic symbionts (with algae
make lichen).
 Secrete powerful hydrolytic enzymes.
 Cell walls contain chitin, an amino sugar polysaccharide
also found in arthropod exoskeletons
 Lack flagella

8. Characteristics of fungi
A. eukaryotic, non- vascular organisms
B. reproduce by means of spores (conidia), usually wind-disseminated
C. both sexual (meiotic) and asexual (mitotic) spores may be
produced, depending on the species and conditions
D. typically not motile, although a few (e.g. Chytrids) have a motile phase.
E. like plants, may have a stable haploid & diploid states
F. vegetative body may be unicellular (yeasts) or multicellular moulds
composed of microscopic threads called hyphae.
G. cell walls composed of mostly of chitin and glucan.
H. Complex cytoplasm with internal organelles, microfilaments and
microtubules

9. H. fungi are heterotrophic ( “other feeding,” must feed on preformed
organic material), not autotrophic ( “self feeding,” make their own
food by photosynthesis).
- Unlike animals (also heterotrophic), which ingest then digest,
fungi digest then ingest.
-Fungi produce exoenzymes to accomplish this
I. Most fungi store their food as glycogen (like animals). Plants store
food as starch.
K. Fungal cell membranes have a unique sterol, ergosterol, which
replaces cholesterol found in mammalian cell membranes
L. Tubule protein—production of a different type in microtubules
formed during nuclear division.
M. Most fungi have very small nuclei, with little repetitive DNA.
N. Mitosis is generally accomplished without dissolution of the
nuclear envelope

10. Introduction of fungi
 Eukaryotic, Heterotrophic
(chemoheterotrophic) microorganism
 No chlorophyll, non-motile
 Thread of cells (hyphae), transverse cell
walls (septate), hyphal anastomosis
 Storage compound; glycogen

11. Molds
 filamentous fungi
 hyphae (s., hypha)
 the filaments of a mold
 may be coenocytic (no cross walls) or have septa
(cross walls)
 mycelium (pl. mycelia)
 bundles or tangled masses of hyphae

12. Yeasts
 unicellular fungi
 reproduce asexually, often by budding
 reproduce sexually by formation of spores

14. Hyphae
 Hyphae are designed to increase the surface area of fungi
and thus facilitate absorption
 May grow fast, up to 1 km per day, as they spread throughout
a food source
 May be coenocytic, having no septa between cells, or septa
may be present with pores through which cytoplasm can flow
moving nutrients through out the fungus
 Parasitic fungi have modified hyphae called
haustoria, which penetrate the host tissue but
remain outside cell membrane

16. Hyphae

17. Hyphae
Septa
Coenocytic
Pores

21. The Body of a Fungus
 Fungi exist mainly in the form of slender
filaments (hyphae).
 long chains of cells joined end-to-end divided by
cross-walls (septa)
 rarely form complete barrier
 cytoplasm freely streams in hyphae
 mycelium - mass of connected hyphae
 grows through and penetrates substrate

22. MYCELIUM
 Intertwined filamentous mass formed by hyphae,
visible to the unaided eye
 Forms when environmental conditions are right
 Vegetative mycelium: Mycelial portion remaining
INSIDE the substrate to obtain nutrition
 Reproductive mycelium: Mycelial portion extends
into air ,responsible for SPORE reproduction

24. mycelia
hypha

25. Introduction of fungi
Other characteristics of fungi
 the ability to synthesize lysine by the -amino
adipic acid pathway (AAA-pathway)
 possession of a chitinous cell wall
 plasma membranes containing the sterol
ergosterol
 and microtubules composed of tubulin.

26. Structure
 Cell wall
 Plasma membrane
 Microtubules
 Nucleus

27. Fungal wall
 Shape of fungi
 Protect against osmotic lysis
 It the wall contains pigments (melanin) 
protect the cell against ultraviolet radiation or
the lytic enzymes of other organisms
 It can have antigenic properties

28. Cell wall components
 Predominance of polysaccharides, lesser
amounts of proteins and lipids

29. Table 1 Major polysaccharide components of
fungal walls
Division
Chytridiomycota
Zygomycota
Ascomycota/
deuteromycota
Basidiomycota
Fibrillar components
Chitin, glucan
Chitin, chitosan
Chitin, (1,3)-(1,6)-
glucans
Chitin, (1,3)- (1,6)-
glucans
Matrix components
Glucan
Polyglucoronic acid,
glucuronomannoproteins
-(1,3)-Glucan, galacto-
mannoproteins ,,

31. Chitin ( N-Acetyl-1-4-β-D- glucosamine

32. Cell wall components
 The major polysaccharides of cell wall matrix
consist of glucans such as manans, chitosan,
and galactans
 Glucan refers to a group of D-glucose polymers
having glycosidic bonds
 Insoluble -glucans are apparently amorphous in cell
wall
 Mannans, galactomannans, rhamnomannans are
responsible for the immunologic response to the
medically important yeasts and molds

33. Cell Wall Structure In Fungi

35. Cell wall components
 Consisting of chitinous microfibrils embedded in
the matrix of small polysaccharides, proteins,
lipids, inorganic salts, and pigments
 Chitin is a (1-4)-linked polymer of N-acetyl-D-
glucosamine (GlcNAc)
 Produced in cytosol (from UDP GlcNAc into
chains of chitin by chitin synthetase)
 The chitin microfibrils are transported to the
plasmalemma and subsequently integrated into
the new cell wall

36. Monosaccharides with taxon association
D-galactose (Ascomycota)
D-galactosamine ( Ascomycota )
L-fucose (Mucorales & Basidiomycota)
D-glucosamine (Mucorales)
D-xylose (Basidiomycota)
Uronic acids (Mucorales)
D-rhamnose (Ascomycota)*
*somewhat rare

37. Cell wall components
 In addition to chitin, glucan, and mannan, cell
walls may contain lipid, protein, chitosan, acid
phosphatase, amylase, protease, melanin, and
inorganic ions (phosphorus, calcium, and
magnesium)
 The outer cell wall of dermatophytes contains
glycopeptides that may evoke both immediate
and delayed cutaneous hypersensitivity

38. Plasma membrane
 The main role of the plasma membrane
 To regulate the uptake and release of materials
 Integral membrane protein (chitin syntase,
glucan syntase)
 Signal transduction

39. Plasma membrane
 Similar to mammalian plasma membrane,
differing in having the nonpolar sterol
ergosterol, rather than cholesterol
 regulates the passage of materials into and
out of the cell by being selective permeable
 Several antifungal agents interfere with
ergosterol synthesis (i.e., amphotericin B)

41. Microtubules
 Composed of the protein tubulin, which
consists of a dimer composed of two
protein subunits.
 Microtubules are long, hollow cylinders ~
25 nm in diameter
 Involved in the movement of organelles,
chromosomes, nuclei, and Golgi vesicle
containing cell wall precursor

42. Microtubules
 Assist in the movement of chromosomes
during mitosis and meiosis
 the destruction of cytoplasmic microtubules
interferes with the transport of secretory
materials to the cell periphery, which may
inhibit cell wall synthesis

43. Nucleus
 The nucleus is bounded by a double nuclear
envelope and contains chromatin and a
nucleolus
 Fungal nuclei are variable in size, shape, and
number
 The number of chromosomes varies with the
particular fungus
 S.cerevisiae ; 18 (n)
 T.mentagophytes ; 4 (n)

44. The growth of hyphae
 Apical extension
 Balance between wall
synthesis and wall lysis
 The apical vesicles are
produced from Golgi
bodies and then
transported to the tip
Spitzenkörper (apical body)

45.  The vesicles fuse with the plasma membrane at the tip,
and release their contents.
 enzymes involved in wall synthesis, (chitin syntase,
glucan synthase)
 enzymes involved in wall lysis,
 enzyme activators,
 some preformed wall polymers such as mannoproteins

46. Physiology
 Aeration
 Nutrition
 Water
 Temperature
 Hydrogen ion
 Light

47. Aeration
 The fungi include species that are obligately
aerobic (eg. most Zygomycota), obligately
anaerobic (eg. rumen fungi)
 Organisms can obtain energy by oxidative
(respiratory) metabolism or by fermentation
 O2 is used for oxidative metabolism to generate
energy. However it is essential for biosynthesis
of sterols, unsaturated fatty acids and some
vitamins

48. Table 2 Energy metabolism in relation to O2
requirements
Obligately oxidative. Obligate aerobes. Exp. Rhodotorula
Facultatively fermentative. Energy can be obtained by
oxidative and fermentative processed such fungi are
likely to be faculative anaerobes. Oxidative
metabolism, provides much more energy than
fermentative, so higher yields can occur under aerobic
conditions. Exp. Mucor, Saccharomyces
Obiligately fermentative. Oxygen is not needed for energy
production , may be either harmless or toxic. Exp.
Blastocladia, Neocallimastix

49. Diagrammatic
representation of the
mixed-acid fermentation
of the rumen chytrid
Neocallimastix. Part of
the fermentation occurs
in the cytosol and
hydrogenosome
Hydrogenosome:
functionally
equivalent to the
mitochondria of
aerobic organisms

50. Fungal nutrition
A. Absorptive mode
over whole surface or via restricted absorbing regions ,
e.g. a. rhizoids in "lower" fungi
b. substrate hyphae* in "higher" fungi
c. apical tips of hyphae
*The substrate hyphae of molds nourish the
aerial hyphae and reproductive hyphae
B. Extracellular digestion
Fungi secrete enzymes that depolymerize complex
natural products (proteins, carbohydrates, lipids, etc.) so
they can be absorbed as sources of carbon and energy.

54. The nutrient requirement of fungi
 Carbon needs for the synthesis of
carbohydrates, lipids, nucleic acids, and
proteins.
 Simple sugars, polysaccharides, citric acid, glycerol
 Nitrogen for synthesis of amino acids for
proteins, purines and pyrimidines for nucleic
acids, glucosamine for chitin, and various
vitamins
 Amino acid, ammonium, nitrate

55. Nutrition
 C/N ratio (20:1)
 Other elements
 P : energy-rich compound metabolism,
phospholipid in lipid bilayer
 K : coenzyme
 Mg : concer with sporulation
 S : protein component
 Trace elements
 Fe, Cu, Mn, and Zn

56. Nutrition

57. Czapek-Dox medium
widely used for the culture of fungi
Mineral base:
C and energy source:
N source:
Water:
If a solid medium is
required:
KH2PO4
MgSO4.7H2O
KCl
FeSO4.7H2O
Sucrose (Glu,starch)
NaNO3
Agar
1 g
0.5 g
0.5 g
0.01 g
30 g
2 g
1 litre
20 g

58. Water availability
 Most fungi require very high water availability
(relative humidity), and rapidly dry out or
senescence in dry conditions.
 Water activity (aw) = ps/pw (pure water = 1)
 DNA is denatured at aw = 0.55
 Osmophiles 0.85, Xerophiles 0.80, Halophiles 0.75
 The xerotolerant fungi can grow slowly, at water
activity of 0.64.

59. Temperature

60. Hydrogen ion
 Opt. pH 5.0-7.0
 Acid-tolerant (pH 2.0) Aspergillus,
Penicillium, Fusarium, yeast in stomach of
animals
 Strongly alkaline environment (pH 10-11)
 F.oxysporum, P.variabile

61. Light
 Influence on fungal growth in specific cases
 light does not play a major part in growth
and metabolism of fungi
 A common metabolic effect of light is the
induction of carotenoid biosynthesis

62. Morphology
Yeast
 Unicellular, round or oval, size 8-15 x 3-5 µm
 Conidiogenesis (budding, binary fission, sexual
spores)
Budding
yeasts
Binary fission

64. Morphology
Mold
 Multicellular, hyphae, septate & nonseptate,
hyaline & dematiaceous, diameter 4-20 µm
 Sexual and asexual reproduction
Hyaline aseptate hyphae
Dematiaceous septate hyphae
Hyaline septate hyphae

65. Morphology
Dimorphic fungi (thermally dimorphic fungi)
Environment/Routine culture media
(SDA) 25-300C ---Mold form
Tissue/Enriched media (BHI)
35-370C---Yeast form
Sporothrix schenckii

66. Morphology

67. CLASSIFICATION
Fungi are eukaryotic organisms that do
not contain chlorophyll, but have cell walls,
filamentous structures, and produce spores.
These organisms grow as saprophytes and
decompose dead organic matter. There are
between 100,000 to 200,000 species depending on
how they are classified. About 300species are
presently known to be pathogenic for man. There
are five kingdoms of living things. The fungi are
in the Kingdom Fungi.

68. KINGDOM
CHARACTERISTIC
EXAMPLE
Monera
Prokaryocyte
Bacteria
Actinomycetes
Protista
Eukaryocyte
Protozoa
Fungi
Eukaryocyte *
Fungi
Plantae
Eukaryocyte
Plants, Moss
Animalia
Eukaryocyte *
Arthropods
Mammals
Man
*This common characteristic is responsible for the therapeutic dilemma in anti-mycotic therapy.

69. Medically important fungi are in four phyla:
1. Ascomycota - Sexual reproduction in a sack called an ascus
with the production of ascopspores.( Aspergillus, Blastomyces
dermatidis, Histoplasma capsulatus)
2. Basidiomycota -Sexual reproduction in a sack called a
basidium with the production of basidiospores.( Cryptococcus
neoformans)
3. Zygomycota - sexual reproduction by gametes and asexual
reproduction with the formation of zygospores.( Rhizopus,
Mucor)
4. Deuteromycota(Fungi Imperfecti Mitosporic Fungi) - no
recognizable form of sexual reproduction. Includes most
pathogenic fungi ( Sporothrix, Coccidioides immitis, Candida,
Pneumocystis).