1. Molecular Biology Seminary Evert Jiménez Medicine 24/08/2010 http://formacionbiblioteca.udea.edu.co/moodle/course/view.php?id=143&topic=1

2. Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer Patients. Ma et al. Journal of Experimental & Clinical Cancer Research 2010, 29:85 http://www.jeccr.com/content/29/1/85 http://formacionbiblioteca.udea.edu.co/moodle/course/view.php?id=143&topic=1

3. INTRODUCTION This study seek determinate the expression of bone morphogenetic protein-2 (BMP-2) and its receptors BMPRIA, BMPRIB, and BMPRII in epithelial ovarian cancer (EOC) and to analyze their influence on the prognosis of ovarian cancer patients.

4. CANCER Cancer is a mutation or abnormal activation of genes that control cell growth and cell mitosis which end in excess of malignant cells with growth and division beyond the normal limits. http://www.medicosdeelsalvador.com/Detailed/Im_genes_M_dicas/Coloproctolog_a/C_ncer_de_Ovario_946.html

5. OVARIO Is the Gonad femenine. is responsible for produce female sex hormones and ovulation. There are two with almond form. They connect with the uterus through the fallopian tubes and suspended by the broad ligament of ovary . http://www.ferato.com/wiki/index.php/Ovario

6. BMP-2 BMP is a member of the transforming growth factor-β superfamily. Initially, it was thought to induce bone formation and condrogenesisin vivo, and current evidence suggests that it also participates in some biological processes of cells, such as proliferation, Differentiation and apoptosis. http://www.treslacteospordia.org.uy/alternativas_chicos.php

7. GENERAL OBJETIVE The propose of this study is determinate the expression of BMP-2 and its receptors in epithelial ovarian cancer, benignovariantumors, and normal ovarian tissue and to analyze their influence on the five-year survival rate and average survival time of ovarian cancer patients.

8. MATERIALES Y METODOS RT-PCR Western Blot INMUNOHISTOQUIMICA

9. MATERIALES Y METODOS RT-PCR Western Blot RNA total extraido de 100mg especimenes 1.5% agarosa gel electroforesis con Bromuro de etidio. la proteína fue extraída de 100 mgespecímenes INMUNOHISTOQUIMICA fueron analizadas con el método de Bradford Poliacrilamida gel electroforesis Parafina Filtro de nitrocelulosa Anticuerpos

10. RESULTADO Los niveles de expresión del ARNm de BMP-2, BMPRIB, y BMPRII en los tejidos de cáncer de ovario fue significativamente menor que los de los tumores benignos ováricos o normal tejido. No hubo diferencias significativas en BMPRIA ARNmniveles de expresión que se observaron entre los tres tipos de Tejidos. RT-PCR El contenido relativo de las proteínas BMP-2, BMPRIB, yBMPRII en el tejido de cáncer de ovario fue significativamente menorque los de los tumores benignos ováricos o normaltejido. No hubo diferencias significativas en proteínas BMPRIAnivel de expresión se observaron entre los tres tipos deTejidos. Western Blot INMUNOHISTOQUIMICA

11. Figure 1 The mRNA expression of BMP-2 and its receptors detected by RT-PCR 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue; M: Marker.

12. 1 3 2 B-actin 43KD BMP-2 20KD BMRIA 66KD BMRIB 50KD BMRII 75KD 2 The protein expression of BMP-2 and its receptors detected by western blot 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue.

13. DISCUSsION http://churchwhisperer.com/2009/09/01/after-much-discussion/

15. CONCLUSION

17. THANK`S http://www.valledellili.org/sitio/index.php?option=com_content&view=article&id=446%3Aen-el-aparato-reproductor-tambien-hay-riesgo&catid=38%3Anuestras-noticias&Itemid=112&lang=es