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poster
1. Bioceramics from shark teeth with potential applications in bone tissue engineering
E. Balboa, P. Coladas, C. Rodríguez-Valencia, M. López-Álvarez, J. Serra, P. González
Grupo Nuevos Materiales, Dpto. de Física Aplicada, Instituto de Investigación Biomédica de Vigo (IBIV), Universidade de Vigo
Campus Lagoas-Marcosende 36310 Vigo, Spain
jserra@uvigo.es
BONE TISSUE
ENGINEERING
BIOINSPIRED
MATERIALS OBTAINING OF SHARK
TEETH AS A FISHING
BYPRODUCT
SHARK TEETH
SEM
TEM
MICRO-X RAY SCANNING (µ-CT) + MECHANICAL TESTS
Enameloid
Dentine
Enameloid (FA) is much
more crystalline than
dentine (HA) XRD
NATURAL SHARK TEETH
CHARACTERIZATION
Ball Milling
Pyrolyzation: 950ºC, 10h
Sieving
20 to 63 μm
Chemical composition is retained
FT-Raman
Pyrolyzation sharply increases
crystallinity
XRD
Optimal cell attachment and healthy proliferation
PROCESSING METHOD
BIOCOMPATIBILITY
PYROLYZED GRANULATE
ACKNOWLEDGEMENTS
BIOLOGICAL
APATITE
NANOCRYSTALS
GRANULES
• Biological apatites have been obtained by the
valorisation of a fishing by-product.
• Apatite granulated and isolated faceted fluorapatite
nanocrystals were obtained by a pyrolysis process.
• Optimal cell attachment and healthy proliferation
have been demonstrated by a preliminary
biocompatibility test up to 21 days.
CONCLUSIONS
0
0,5
1
1,5
2
7 21
Absorbance(A595-A655)
Incubation time (days)
TCP
HA shark
Ti
*
*