67. DynamicImagingofgenomicloci
Chen et al., Cell, 2013, Dynamic imaging of genomic loci in living human cells
by an optimized CRISPR/Cas system
AttachedaGFPtoanuclease-deficient Cas9(dCas9)
21
68. DynamicImagingofgenomicloci
Chen et al., Cell, 2013, Dynamic imaging of genomic loci in living human cells
by an optimized CRISPR/Cas system
AttachedaGFPtoanuclease-deficient Cas9(dCas9)
21
69. DynamicImagingofgenomicloci
Chen et al., Cell, 2013, Dynamic imaging of genomic loci in living human cells
by an optimized CRISPR/Cas system
AttachedaGFPtoanuclease-deficient Cas9(dCas9)
21
77. Geneticallymodifyhumanembryos
Liang et al., Protein Cell, 2015, CRISPR/Cas9-mediated
gene editing in human tripronuclear zygotes
Triedtomutatethehumanβ-globin(HBB)genein‘non-viable’
embryos(β-thalassaemia)
23
78. Geneticallymodifyhumanembryos
Liang et al., Protein Cell, 2015, CRISPR/Cas9-mediated
gene editing in human tripronuclear zygotes
Triedtomutatethehumanβ-globin(HBB)genein‘non-viable’
embryos(β-thalassaemia)
7of86embryosweresuccessfullymutated
Muchmorehigherratesofoff-targeting
23
79. Geneticallymodifyhumanembryos
Liang et al., Protein Cell, 2015, CRISPR/Cas9-mediated
gene editing in human tripronuclear zygotes
Triedtomutatethehumanβ-globin(HBB)genein‘non-viable’
embryos(β-thalassaemia)
7of86embryosweresuccessfullymutated
Muchmorehigherratesofoff-targeting
Raisehugeethicalconcerns…
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