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Development of platform techniques to produce cheap, ecofriendly and easy-to-use diagnostic assays for livestock diseases
...
Development of platform techniques to produce cheap, eco-friendly
and easy-to-use diagnostic assays for livestock diseases...
The problem: its significance
• What is the state of the problem?
 No or limited diagnostic services, logistic networks a...
Methods/Results-Cheap and eco-friendly
•

Cheap and eco-friendly paperbased diagnostic ELISA (for CBPP)

•

Print 96-well ...
Methods/Results-Cheap and easy-to-use
• The “Diagnostic-Chip” an
easy-to-use point-of-care
diagnostics tests
• Simultaneou...
Methods/Results-Cheap and easy-to-use
•

Silicon-cartridge loaded
with primers specific to the
different pathogens

•

Rec...
Methods/Results-Cheap and easy-to-use

The electronic manifold contains electronics for fluorescent detection
and temperat...
Methods/Results-Cheap and easy-to-use

8
Conclusions and discussion points
• What have you discovered?
We are in the process of establishing a diagnostic technolo...
Where to from now?
• How do you see the next step in this project?
 Proceed with the development of the diagnostic platfo...
Where to from now, continued?
• With whom (at ILRI and in the world, university, research
center, etc) do you intend to wo...
General discussion-JJ
• We need a credit card linked to our grants to make
our orders, since we loose constantly many mont...
General discussion-JJ
•Get the PIs involved in making decisions

•Create a system rewarding scientists that are
performing...
better lives through livestock
ilri.org

The presentation has a Creative Commons licence. You are free to re-use or distri...
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Development of platform techniques to produce cheap, eco-friendly and easy-to-use diagnostic assays for livestock diseases

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Presented by Anne Liljander, Nimmo Gicheru, Cecilia Muriuki and Joerg Jores at the ILRI BioSciences Day, Nairobi, 27 November 2013



Publié dans : Technologie, Santé & Médecine
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Development of platform techniques to produce cheap, eco-friendly and easy-to-use diagnostic assays for livestock diseases

  1. 1. Development of platform techniques to produce cheap, ecofriendly and easy-to-use diagnostic assays for livestock diseases Anne Liljander, Nimmo Gicheru, Cecilia Muriuki and Joerg Jores ILRI BioSciences day, Nairobi, 27 November 2013
  2. 2. Development of platform techniques to produce cheap, eco-friendly and easy-to-use diagnostic assays for livestock diseases • Introduction • Why is this research important for ILRI  Setup of a diagnostic platform technology  Cutting edge research in bioscience • Who are the recipients of the outcomes  Stakeholders in the livestock sector i.e. livestock keepers, traders, national veterinary services, veterinarians and animal health workers • How will it change their lives  Improved nutrition and health  Increased profit for livestock keepers 2
  3. 3. The problem: its significance • What is the state of the problem?  No or limited diagnostic services, logistic networks and infrastructure available in most parts of the country  There are no cheap POC diagnostics available • How will this project change the outcomes?  Enable diagnosis of diseases in areas with no or limited infrastructure  Enable the implementation of control measures, reduce disease burden and increase productivity of the livestock sector • What are the links to ILRI’s strategy (SLOs and Strategic Outcomes)?  Reduced rural poverty (SLO1)  Improved food security (SLO2)  Improved nutrition and health (SLO3) 3
  4. 4. Methods/Results-Cheap and eco-friendly • Cheap and eco-friendly paperbased diagnostic ELISA (for CBPP) • Print 96-well paper “plate” using regular printer and wax-based ink • Use in “regular” ELISA experimental setup • Production process 1. Print the plate 2. Melt the wax Saves money, can be produced anywhere and shipped by regular post 3. Use in assay 4
  5. 5. Methods/Results-Cheap and easy-to-use • The “Diagnostic-Chip” an easy-to-use point-of-care diagnostics tests • Simultaneous identification of various pathogens • Portable silicon-cartridge, plugs into a smartphone for use in resource-limited environments 5
  6. 6. Methods/Results-Cheap and easy-to-use • Silicon-cartridge loaded with primers specific to the different pathogens • Recombinase polymerase amplification (RPA) of DNA at 37◦C in 10-15 min • Sample is loaded, the heated reaction and the fluorescence detection are powered by a smartphone 6
  7. 7. Methods/Results-Cheap and easy-to-use The electronic manifold contains electronics for fluorescent detection and temperature control 7
  8. 8. Methods/Results-Cheap and easy-to-use 8
  9. 9. Conclusions and discussion points • What have you discovered? We are in the process of establishing a diagnostic technology platform at ILRI which will substantially accelerate the diagnosis of diseases and epidemiological studies • How will you use it? Better diagnostics are key instruments for improved control and eradication programs (vaccination campaigns) and setup of policies • What are the next challenge? Roll out the technology with the help of integrated sciences
  10. 10. Where to from now? • How do you see the next step in this project?  Proceed with the development of the diagnostic platform technology  Find appropriate partners for the roll out the technology • How will it contribute to the four CGIAR System Level Outcomes (Reduce poverty, improve nutrition, improve food security and enhance environment) and link with CRP/value chains?     Reduced rural poverty (SLO1) Improved food security (SLO2) Improved nutrition and health (SLO3) CRP 3.7 and 4.3, Dairy value chain in Tanzania, small ruminants in Mali and Ethiopia
  11. 11. Where to from now, continued? • With whom (at ILRI and in the world, university, research center, etc) do you intend to work with? Do you have grant applications about it? Partners: Steve Kemp/Yumi (ILRI), UoW-Madison, FLI, ICIPE, UoB  Funded by Centrum fur Internationale Migration and Entwicklung (CIM) from 1/1/2014-31/12/2015  New GIZ/BMZ grant  Old GIZ/BMZ grant  Swiss Agency for Development and Cooperation (SDC)-One Health – pending  Swiss National Science Foundation (NSF)-pending  I would like to submit a BMC small grant proposal on user acceptance of novel POC tests next year
  12. 12. General discussion-JJ • We need a credit card linked to our grants to make our orders, since we loose constantly many months of productive working time because of excessive delays in orders • Make sure that ILRI services are pro-active and feedback to scientists • Do not make budgetary decisions without involving the PIs (per diem adjustments without consultations of PIs) 12
  13. 13. General discussion-JJ •Get the PIs involved in making decisions •Create a system rewarding scientists that are performing well in resource mobilization and publications (bioscience specific) •Reduce ILRI costs to enable growth outside the CRPs 13
  14. 14. better lives through livestock ilri.org The presentation has a Creative Commons licence. You are free to re-use or distribute this work, provided credit is given to ILRI.

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