methods of culture.pptx

1. Methods of culture

2. Methods of culture
◌Streak culture
◌Lawn or carpet culture
◌Stroke culture
◌Stab culture
◌Pure plate culture
◌Liquid culture.

3. Methods of culture Streak culture
◌Surface plating
◌Routinely employed for the isolation of bacteria in pure culture

4. • https://www.youtube.com/watch?v=_1KP9zOtjXk
• https://www.youtube.com/watch?v=DMz54_YaZyc

5. Methods of culture Streak culture
◌ A platinum loop with 2.5 “ long wire and loop with diameter 2mm
◌ Charged with specimen to be cultured and is placed on the surface of
Dried plate of solid media towards peripheral area
The inoculum is spread thinly over the plate in series of parallel lines in different
segment of the plate.
On incubation we may find confluent growth at the site of primary inoculum
Well separated colonies are obtained over the final series of streaks

6. Methods of culture lawn or carpet culture
◌Lawn cultures are prepared by flooding the surface or plate with
suspension of bacteria
◌It provides uniform surface growth of bacteria
◌It is useful for bacteriophage typing and antibiotic sensitivity

7. Methods of culture lawn or carpet culture
◌https://www.youtube.com/watch?v=oZHIrh8FD9k

8. Methods of culture Stroke culture
◌It is made in tubes containing agar slopes
◌It is used for providing a pure growth of bacterium for slide
agglutination

9. Methods of culture Satb culture
◌It is prepared by puncturing with charged long, straight wire (4” long)
◌Stab culture are employed mainly for demonstration of gelatin
liquefactions and for maintaining stock culture
◌https://www.youtube.com/watch?v=bNvrs2FKYl8
◌

10. Methods of culture Pour Plate culture
◌ 15ml of agar medium is melted and left to cool in water at 45 to 50*C
◌ Appropriate of dilution of inoculum is added in 1ml volume to molten agar and
mixed well
◌ Content of tube is poured in petri dish
◌ It is allowed to set and after incubation colonies will be seen distributed throughout
the depth of medium
◌ This method gives viable bacterial count in a suspension
◌ Recommended method for quantitative urine culture

11. Methods of culture Pour Plate culture
◌https://www.youtube.com/watch?v=lnfcRyr9wYU

12. Methods of culture Liquid culture
◌In a tube , bottle or flask may be inoculated by touching with a
charged loop.
◌https://www.youtube.com/watch?v=hw0IFzXSV0c