1. Jonathon
N.
Winnay,
Ph.D.
(617)
202-­‐8015/
jonathon.winnay@joslin.harvard.edu
h?p://www.linkedin.com/pub/jonathon-­‐winnay/15/842/63
Professional
Overview
• Accomplished
scienJst
with
an
established
record
performing
innovaJve
research
in
mulJple
areas
including
diabetes,
metabolism,
obesity.
hormone
acJon
and
proteostasis.
• EffecJvely
apply
experJse
in
cell
biology,
molecular
biology,
biochemistry
and
physiology
to
perform
and
complete
detailed
mechanisJc
studies
using
both
in
vitro
and
in
vivo
models.
• Proficiency
in
the
design,
implementaJon
and
management
of
mulJple
studies
and
collaboraJons
requiring
effecJve
communicaJon
and
the
coordinaJon
of
staff
and
resources.
• ProacJvely
pursued
enJrely
novel
areas
of
research
that
have
become
the
focus
of
research
within
the
laboratory.
Professional
Experience
Joslin
Diabetes
Center
&
Harvard
Medical
School,
Boston,
MA.
2006-­‐Present
Post-­‐doctoral
Fellow,
Laboratory
of
C.
Ronald
Kahn.
University
Of
Michigan
Medical
School
&
Pfizer,
Ann
Arbor,
MI
1998-­‐1999
Research
Technician,
Laboratory
of
Alan
Sal?el,
Ph.D.
Joslin
Diabetes
Center
&
Harvard
Medical
School,
Boston,
MA.
1994-­‐1998
Research
Technician,
Laboratory
of
C.
Ronald
Kahn.
Laboratory
Skills
Cell
Biology:
• GeneraJon
of
Stable
Cell
Lines
• Primary
Cell
Culture
• Over-­‐expression
and
knock-­‐down
Cell
Models
• Microscopy/Immunocytochemistry
• Reporter
Assays
• Flow
Cytometry
• Apoptosis
and
ProliferaJon
Biochemistry:
• Recombinant
Protein
ProducJon
• EnzymaJc
Assays
(kinase/phosphatase)
• Affinity
PurificaJon
• Protein
Binding
KineJcs
• InteracJon
Mapping
• Thin
Layer
Chromatography
Molecular
Biology:
• CRISPR/Cas9
Gene
EdiJng
• Recombinant
DNA
ManipulaJon
• Mutagenesis
• Gene
Expression
Analysis
• Immunoblot
Analysis
• ImmunoprecipitaJon
• Transgenic/targeJng
vector
design
• proximity
ligaJon
assay
• Adenovirus,
Retrovirus
&
LenJvirus
ProducJon
Physiology/In
Vivo
• CreaJon
and
characterizaJon
of
animal
models
• EvaluaJon
of
endocrine
pathways
(glucose
metabolism/HPA
axis)
• Immunohistochemistry
• General
Necropsy
• Histology
• Small
Animal
Surgery
• Measurement
of
serum
hormone
and
lipids
• AdministraJon/dosing
(i.p./i.m.)
EducaNon
University
Of
Michigan
Medical
School,
Ann
Arbor,
MI.
(2006)
Ph.D.
in
Integra?ve
Physiology
and
Metabolism,
Laboratory
of
Gary
D.
Hammer,
M.D.,
Ph.D.
Dickinson
College.
Carlisle,
PA.
(1994)
Bachelor
of
Science
(B.S.),
Biology
Management
Experience
Marie
Holm
Solheim,
Doctoral
Candidate,
University
Of
Bergen,
Norway.
(2014-­‐Present)

2. Kishan
Chudasama,
Doctoral
Candidate,
University
Of
Bergen,
Norway.
(2011-­‐2012)
Geoffrey
Rintel;
Undergraduate,
Boston
University.
(2007)
Megan
R.
Hall;
Undergraduate
Research
OpportuniJes
Program,
University
of
Michigan.
(2002-­‐2003)
Grants
and
Awards
Travel
Grant
Recipient,
Keystone
Symposia
(2013)
Mary
K.
Iacocca
Fellowship
(2010-­‐2011)
Pre-­‐doctoral
Fellowship;
Tissue
Engineering
Training
Grant;
University
of
Michigan
Medical
School
(2004-­‐2006)
Departmental
Nominee;
Excellence
in
Teaching
Award;
University
of
Michigan
Medical
School
(2003)
Pre-­‐doctoral
Fellowship;
Cellular
&
Molecular
Approaches
to
IntegraJve
Biology
Training
Grant
(2001-­‐2003)
Honorary
Fellowship;
Joslin
Diabetes
Center/Harvard
Medical
School
(1998)
PublicaNons
1. Cai,
W.,
Sakaguchi,
M.,
Kleinridders,
A.,
Gonzalez-­‐Del
Pino,
G.,
Dreyfuss,
J.,
O’Neill,
B.T.,
Ramirez,
A.K.,
Winnay,
J.N.,
Boucher,
J.,
Eck,
M.J.
and
Kahn,
C.R.
(Accepted)
Domain-­‐dependent
Effects
of
Insulin
and
IGF-­‐1
Receptors
for
Signaling
and
Gene
expression.
Nat
Commun.
2. Thomou,
T.,
Mori,
M.,
Dreyfuss,
J.,
Rao,
T.,
Winnay,
J.N.,
Grinspoon,
S.,
Gorden,
P.,
Kahn,
C.R.
(Accepted)
Adipose
Tissue
is
a
Major
Source
of
CirculaJng
microRNAs
That
Can
Regulate
Gene
Expression
in
Other
Tissues.
Nature
3. Solic,
S.,
Boucher,
J.,
Solheim,
M.H.,
Fujisaka,
S.,
Haering,
M.,
Homan,
E.P.,
Winnay,
J.N.,
Perez-­‐Atayde,
P.,
Kahn,
C.R.
Lipodystrophy
Due
to
Adipose
Tissue
Specific
Insulin
Receptor
Knockout
Results
in
Progressive
NAFLD.
Diabetes.
2016
Aug;65(8):2187-­‐200..
4. Winnay,
J.N.,
Solheim,
M.,
Dirice,
E.,
Molven,
A.,
Njolstad,
P.R.,
Kahn,
C.R.
(2016)
PI3-­‐kinase
mutaJon
linked
to
insulin
and
growth
factor
resistance
in
vivo.
J
Clin
Invest.2016
Apr
1;126(4):1401-­‐12.
5. Zhang
H.,
Guan
M.,
Huang
T.L.,
Townsend
K.L.,
An
D.,
Yan
X.,
Schultz
T.J.,
Winnay
J.N.,
Mori
M.,
Hirshman
M.F.,
KrisJansen
K.,
Tsang
J.,
Goodyear
L.J.,
and
Tseng
Y.
(2015)
MicroRNA-­‐455
Regulates
Brown
Adipogenesis
via
a
Novel
Signaling
Network.
EMBO
Rep.
Oct;
16(10):1378-­‐93.
6. Winnay,
J.N.,
Dirice,
E.,
Kulkarni,
R.N.,
Kahn,
C.R.
(2014)
p85α
Deficiency
Protects
β-­‐cells
from
ER
Stress
Dependent
Apoptosis.
Proc
Natl
Acad
Sci.,
Jan
21;111(3):1192-­‐7.
7. Lee
KY,
Yamamoto
Y,
Boucher
J,
Winnay,
J.N.,
Gesta
S,
Cobb
J,
Bluher
M,
Kahn
CR.
(2013)
Shox2
is
a
molecular
determinant
of
depot-­‐specific
adipocyte
funcJon.
Proc
Natl
Acad
Sci.,
Jul
9;110(28):11409-­‐14.
8. Chudusama,
K.K.*,
Winnay,
J.N.*,
Aarskog,
D.,
Claudi,
T.,
Haldorsen,
I.,
Johansson,
S.,
Konig,
R.,
Molven,
A.,
Sagen,
J.,
Woo,
J.,
Shoelson,
S.,
Kahn,
C.R.,
Njolstad,
P.R.
(2013)
SHORT
Syndrome
with
ParJal
Lipodystrophy
Due
to
Impaired
PI
3-­‐kinase
Signaling.
Am.
J.
Hum.
Genet.,
Jul
11;93(1):150-­‐7.
9. Park
K,
Li
Q,
Rask-­‐Madsen
C,
Mima
A,
Mizutani
K,
Winnay
J.N.,
Maeda
Y,
D'Aquino
K,
White
MF,
Feener
EP,
King
GL
(2013)
Serine
PhosphorylaJon
Sites
on
IRS2
by
Angiotensin
II
and
PKC
AcJvated
to
Induce
SelecJve
Insulin
Resistance
in
Endothelial
Cells.
Mol
Cell
Biol.
Aug;
33(16):3227-­‐41.
10. Palsgaard,
J.,
Emanuelli,
B.,
Winnay,
J.N.,
Sumara,
G.,
Karsenty,
G.,
Kahn,
C.R.
(2012)
Crosstalk
between
insulin
and
Wnt
signaling
in
preadipocytes:
Role
of
Wnt
Co-­‐receptor
LDL
receptor
related
protein-­‐5
(LRP5)
J.
Biol.
Chem.
287(15):
12016-­‐26.
11. Bezy,
O.,
Tran,
T.,
Suzuki,
R.,
Emanuelli,
B.,
Winnay,
J.N.,
Mori,
M.,
Haas,
J.,
Leitges,
M.,
King,
G.L.,
Kahn,
C.R.
(2011)
PKCδ
Serves
as
a
GeneJc
Regulator
of
HepaJc
Insulin
SensiJvity
and
Risk
of
Hepatosteatosis
Between
Mouse
Strains.
J
Clin
Invest
121:2504-­‐17
12. Taniguchi,
C.
M.,
Winnay,
J.N.,
Kondo,
T.,
Bronson,
RT.,
Guimaraes,
A.R.,
Aleman,
J.O.,
Luo,
J.,
Stephanopoulos,
G.,
Weissleder,
R.,
Cantley,
L.C.,
and
C.
R.
Kahn.
(2010)
The
PhosphoinosiJde
3-­‐Kinase
Regulatory
Subunit
p85α
Can
Exert
Tumor
Suppressor
ProperJes
through
NegaJve
RegulaJon
of
Growth
Factor
Signaling.
Cancer
Res.
70(13):
5305-­‐15.
13. Winnay,
J.N.,
Boucher,
J.,
Mori,
M.A.,
Ueki,
K.,
Kahn,
C.R.
(2010)
A
Regulatory
Subunit
of
PhosphoinosiJde
3-­‐kinase
Increases
the
Nuclear
AccumulaJon
of
X-­‐box–binding
protein-­‐1
to
Modulate
the
Unfolded
Protein
Response.
Nat.
Med.
16(4):
438-­‐45.
14. Sopasakis,
V.
R.,
Liu,
P.,
Suzuki,
R.,
Kondo,
T.,
Winnay,
J.,
Tran,
T.T.,
Asano,
T.,
Smyth,
G.,
Sajan,
M.P.,
Farese,
R.V.,
Kahn,
C.R.,
and
Zhao,
J.J.
(2010)
Specific
Roles
of
the
p110alpha
Isoform
of
PhosphaJdylinsositol
3-­‐Kinase
in
HepaJc
Insulin
Signaling
and
Metabolic
RegulaJon.
Cell
Metab.
11:220-­‐230.

3. 15. Assmann,
A.,
Ueki,
K.,
Winnay,
J.N.,
Kulkarni,
R.N.
(2009)
Glucose
effects
on
beta-­‐cell
growth
and
survival
require
acJvaJon
of
insulin
receptors
and
insulin
receptor
substrate-­‐2.
Mol
Cell
Biol.
29
(11):
3219-­‐28.
16. Tseng
YH,
Kokkotou
E,
Schulz
TJ,
Huang
TL,
Winnay
J.N.,
Taniguchi
CM,
Tran
TT,
Suzuki
R,
Espinoza
DO,
Yamamoto
Y,
Ahrens
MJ,
Dudley
AT,
Norris
AW,
Kulkarni
RN,
Kahn
CR.
(2008)
New
role
of
bone
morphogeneJc
protein
7
in
brown
adipogenesis
and
energy
expenditure.
Nature
Aug
21;
454
(7207):
1000-­‐4.
17. Jonathon
N.
Winnay
and
Gary
D.
Hammer
(2006)
ACTH-­‐mediated
signaling
cascades
coordinate
a
cyclic
pa?ern
of
SF-­‐1-­‐dependent
transcripJonal
acJvaJon.
Mol.
Endocrinol.
(2006)
Jan;
20(1):147-­‐66.
18. Winnay,
J.N.,
Xu,
J.,
O’Malley,
B.
and
Hammer,
G.D.
(2006)
SRC-­‐1-­‐deficient
mice
exhibit
altered
endocrine
funcJon
at
mulJple
levels
of
the
hypothalamic-­‐pituitary-­‐adrenal
axis.
Endocrinology
Mar;
147(3):
1322-­‐32.
19. Kulkarni,
R.N.,
Jhala,
U.S.,
Winnay,
J.N.,
Krawjewski,
S.,
MonJminy,
M.R.
and
Kahn,
C.R.
(2004)
PDX-­‐1
Haploinsufficiency
Limits
the
compensatory
Islet
Hyperplasia
that
Occurs
in
Response
to
Insulin
Resistance.
J.
Clin.
Invest.
Sept.;
114(6):
828-­‐36
20. Ross,
S.
E.,
Radomska,
H.
S.,
Wu,
B.,Zhang,
P.,
Winnay,
J.N.,
Bajnok,
L.,
Wright,
W.,
Schaufele,
F.,
Tenen,
D.
G.
and
MacDougald,
O.
A.
(2004)
PhosphorylaJon
of
C/EBPα
inhibits
granulopoiesis.
Mol.
Cell.
Biol.
24(2):
675-­‐86.
21. Gummow,
B.M.,
Winnay,
J.N.
and
Hammer,
G.D.
(2003)
Convergence
of
Wnt
Signaling
and
SF-­‐1
on
TranscripJon
of
the
Rat
Inhibin
alpha
Gene.
J.
Biol.
Chem.
278:26572-­‐26579.
22. Kulkarni,
R.N.,
Almind,
K.,
Goren,
J.H.,
Winnay,
J.N.,
Ueki,
K.
and
Kahn,
C.R.
(2003)
Impact
of
GeneJc
Background
on
Development
of
Hyperinsulinemia
and
Diabetes
in
Insulin
Receptor/Insulin
Receptor
Substrate-­‐1
Double
Heterozygous
Mice.
Diabetes
52(6):
1528-­‐1534.
23. Mauvais-­‐Jarvis,
F.,
Virkamaki,
A.,
Michael,
M.D.,
Winnay,
J.N.,
Zisman,
A.,
Kulkarni,
R.H.
and
Kahn,
C.R.
(2000)
A
Model
to
Explore
the
InteracJon
Between
Muscle
Insulin
Resistance
and
β-­‐cell
DysfuncJon
in
the
Development
of
Type
2
Diabetes.
Diabetes
49:2126-­‐2134.
24. Winnay
J.N.,
Bruning
J.C.,
and
Kahn
C.R.
(2000)
Gab-­‐1
Mediated
IGF-­‐1
Receptor
Signaling
in
the
Absence
of
Tyrosine
PhosphorylaJon.
J.
Biol.
Chem.
275:10545-­‐10550.
25. Kulkarni
R,
Winnay
J.N.,
Daniels
M.,
Bruning
J.C.,
Flier
S.,
Hanahan
D.,
and
Kahn
C.R.
(1999)
Heterogeneous
Glucose-­‐
SJmulated
Insulin
SecreJon
in
IRS-­‐1
Deficient
Mouse
Islets
and
Clonal
β-­‐cell
Lines.
J.
Clin.
Invest.
104:R69-­‐R75.
26. Kulkarni
R.,
Bruning
J.C.,
Winnay
J.N.,
PosJc
C.,
Magnuson
M.,
and
Kahn
C.R.
(1999)
Tissue-­‐Specific
Knockout
of
the
Insulin
Receptor
in
PancreaJc
β-­‐cells
Creates
an
Insulin
Secretory
Defect
Similar
to
that
in
Type
2
Diabetes.
Cell
96:329-­‐339.
27. Bruning
J.C.*,
Michael
M.D.*,
Winnay
J.N.*,
Tatsuya
H.,
Horsch
D.,
Accili
D.,
Goodyear
L.J.,
and
Kahn
C.R.
(1998)
A
Muscle-­‐
Specific
Insulin
Receptor
Knockout
Exhibits
Features
of
the
Metabolic
Syndrome
of
NIDDM
without
Altering
Glucose
Tolerance.
Mol.
Cell
2:559-­‐569.
28. Bruning
J.C.,
Winnay
J.N.,
Bonner-­‐Weir
S.,
Taylor
S.I.,
Accili
D.,
and
Kahn
C.R.
(1996)
Development
of
a
Novel
Polygenic
Model
for
NIDDM
in
Mice
Heterozygous
for
Insulin
Receptor
and
IRS-­‐1
Null
Alleles.
Cell
88:561-­‐572.
29. Bruning
J.C.,
Winnay
J.N.,
Cheatham
B.,
and
Kahn
C.R.
(1996)
DifferenJal
Signaling
by
IRS-­‐1
and
IRS-­‐2
in
IRS-­‐1
Deficient
Cells.
Mol.
Cell.
Biol.
17:1513-­‐1521.
*These
authors
contributed
equally
to
this
work.
Reviews/Book
Chapters
1. Jonathon
N.
Winnay
and
C.
Ronald
Kahn
(2011)
PI
3-­‐Kinase
Regulatory
Subunits
as
Regulators
of
the
Unfolded
Protein
Response.
Methods
Enzymol.
490:147-­‐58.
2. Bruning,
J.C.,
Winnay,
J.N.,
Bonner-­‐Weir,
S.,
Accilli,
D.,
Kahn,
C.R.
(1996)
CreaJng
Polygenic
Models
of
Insulin
Resistance
and
Diabetes
Mellitus.
J.
of
Invest.
Med.
44:A258.