1. PROFESSIONAL EXPERIENCEPROFESSIONAL EXPERIENCE
Aug 2007-May 2015: Research scientist, V-region discovery and engineering group (VRDE),
UCB (Slough, UK)
 Antibodies humanisation and murinisation campaign, for therapeutic projects : in-silico
humanisation, cloning in vectors, expression in mammalian cells, data analysis for
selection of best candidates from various assays
 Additional role from Oct 2013 to May 2015 in the HS&E team as Lab steward and
organisation of various HS&E campaigns
June 2004-August 2007: Research associate, molecular biology group, GLYCART Biotechnology
(Zurich, Switzerland, company acquired by ROCHE in Jan 2006)
 Development of antibody expression vectors and transient expression evaluation in mammalian cells
 Production and purification of several His-tag proteins for immunization purposes and testing of antisera
Sept 2003-May 2004: Research associate, NOVARTIS (Horsham, UK)
 Identification of novel therapeutic targets for the development of asthma treatments
SKILLSSKILLS
 Molecular & Cellular biology: cloning, PCR & mutagenesis, DNA/RNA purification, culture and
transformation/transfection of bacteria and mammalian cells, isolation and cloning of antibodies from
hybridoma, use of molecular biology softwares
 Biochemistry: ELISA, Octet, SDS-Page, western-blotting, His-tag protein purification,
immunofluorescence, immunoprecipitation, metabolic labelling
 Languages : native French, fluent in English, basic knowledges in Italian and German
EDUCATIONEDUCATION
Sep 2000-Sep 2003 : Master's Degree in Biotechnology (E.S.I.L : École Supérieure d’Ingénieur de
Luminy, Aix-Marseille University, France)
• Active member of the student association Prov’ing (2002&2003) : member of the organizing committee of the scientific
seminar “Diagnostic in Biotechnology”, a two-days meeting and debate between students, academic researchers
and private companies
Sep 1998-Sep 2000 : Brevet de Technicien Supérieur en Biotechnologie; equivalent to 2-years
technical degree in Biotechnology (Lycée Technique Supérieur Marie-Curie, Marseille, France)
TRAINING PERIODSTRAINING PERIODS
Feb-Aug 2003: Research Associate, Novartis (Horsham, UK): Master's degree final year internship
• Generation of a customized Baculovirus-mediated Drosophila S2 cells protein expression system
Feb-March 2002: Research technician, Immunology Center of Marseille-Luminy (Research Unit INSERM-CNRS,
France)
• Study of VPREB3 protein (protein involved in B cells maturation) → Characterization of monoclonal antibodies and a
transfected lymphocyte cell line
1999-2000: Research technician at the INSERM Unit 464 - Ion Channels Neurobiology ( Marseille Faculty of
Medicine,, France)
• Jan-Feb 2000: In vivo reconstitution of synaptotagmin/voltage-dependent sodium channel interaction
→ Generation of transfected cell lines expressing the protein of interest and interaction characterization
• June 1999: Voltage-dependent sodium channel neuronal expression study
→ Plasmidic expression vectors generation, culture of neuronal cells extracted from rat brain
Karine HERVÉ
(+1) 778-877-7584
kween1081@yahoo.fr

2. Patents & PublicationPatents & Publication
 Publication:
A mixture of functionally oligoclonal humanized monoclonal antibodies that neutralize clostridium difficle
TcdA and TcdB with high levels of in vitro potency shows protection in a hamster infection model.
Davies NL1, Compson JE, Mackenzie B, O'Dowd VL, Oxbrow AK, Heads JT, Turner A, Sarkar K, Dugdale SL, Jairaj M,
Christodoulou L, Knight DE, Cross AS, Hervé KJ, Tyson KL, Hailu H, Doyle CB, Ellis M, Kriek M, Cox M, Page MJ, Moore AR,
Lightwood DJ, Humphreys DP. Clin Vaccine Immunol. 2013 Mar;20(3):377-90
 Patents:
WO2013/038156
NEUTRALISING ANTIBODIES TO THE MAJOR EXOTOXINS TCDA AND TCDB OF CLOSTRIDIUM DIFFICILE
WO2015/028455 A1
Anti CSF1-R ANTIBODIES AND BINDING FRAGMENTS