1. How to use Genstat for mean
comparision?
Prepared By
Krishna Dhakal
M.Sc.Ag
Genetics and Plant Breeding
Faculty of Agriculture
Agriculture and Forestry University
Rampur, Chitwan, Nepal
Final work: March 13, 2017
2. A simple guideline
First of all you make sure that your data is correctly entered in excel
sheet
Select and copy the data from the excel sheet (make sure you select
and copy all the data)
Bring the icon of Genstat on your desktop
Now, open your Genstat desktop icon
Go to “tools”---”Option”---”menu”---choose “show multiple mean
comparison on ANOVA menu” and click “OK”
Click on spread…..New….. then “from clipboard”
A small window of new spreadsheet will appear, Click “yes” on
“column name on first row” , that will show the heading of your data
table
7. To load data with its headings click on
“yes” of the “column names in first row”
8. Continued….
Now, in the left side of your Screen data will be seen and in right side
small window will appear where you have to fix which one is factor
and which one is the variables and click “OK”
Replication, block, varieties etc are factors and plant height, yield, etc
are variables
you can denote it by double clicking on the shown options
When the work is done, a red colored exclamation mark (!) appears on
the concerned subject, if you wrongly fixed the factors, you can again
change it by clicking in the concerned subject and correct it
12. For single factor comparision
You have now fixed the factors, its time for mean comparison
Go to “stat” click on “analysis of variance” choose “General” and
choose the design of your experiment, load “Y” variate and treatment
and click “Run”
To view the results, go to “window” click on “output” and see the
results
18. For two factor mean comparison
(RCBD)
The process is similar to one factor mean comparison
The difference occurs while choosing Design of experiment, and other
options according to your interest
When you choose two way RCBD then the small window of analysis of
variance would appear as follows
21. Continue…..
In the above slide you can see “option” in the right side of “run”, you
can use that for desirable setting of your analysis
For example: you would like to obtain CV, standard errors of means
and other too based on the requirement for your analysis
Also you can go to “multiple comparison” option that is available in
ANOVA options and set the desirable changes, the ANOVA options is
shown in the below slide
24. Continued……
From multiple comparison options you can choose either variety or
environment or variety and environment interaction as a treatment to
compare the means.
Also you can choose whether you want the results of means comparison
in ascending or descending order.
You can also choose the type of test you wanted to choose.
After choosing, click on “OK” in both multiple comparison and ANOVA
options mini window
Click “Run” and then observe the output clicking “window” and
selecting “output”
26. Split plot design
The similar is the procedure like in two factorial RCBD
The only difference occurs while choosing the Design of experiment also
the desired treatment interaction should be given that is interaction may
be main plot treatment Vs sub plot treatment or subplot Vs main plot.
Here in example either environment or treatment can be taken as main
plot and the variety as sub plot treatment or variety as main plot or
environment as subplot treatment.
Accordingly the treatment interaction will be env × var or var × env
Click “Run” and then observe the output clicking “window” and
selecting “output”
28. Strip plot design
The similar is the procedure like in split plot design
The only difference occurs while choosing the Design of experiment
and the interaction between row and column should be designed
That means interaction treatment may be between row and column or
column and row
Click “Run” and then observe the output clicking “window” and
selecting “output”
