1. Purifier® Logic® Class II Biological Safety Cabinet

2. COPYRIGHT NOTICE
The material included in this presentation is
copyright of Labconco Corporation.
Reproduction or use of the slides is not
permitted without express permission from
Labconco Corporation.

3. Fume Hood or Biological Safety Cabinet?
Fume Hoods - Enclosures that capture,
contain and remove chemical fumes
and vapors.
Biological Safety Cabinets -
Enclosures that capture, contain and
remove biohazardous aerosols.

4. Purifier® Logic® Class II Biological Safety Cabinet
Optional 254 nm UV lamp Contain-Air Negative Pressure
Channel
Bright, glare-free fluorescent
lighting
Innovative LCD information center
display
Fully-closing, counterbalanced
tempered safety glass sash
Easy-to-clean touchpad controls
Large 21.7” sash opening height Reserve-Air Secondary Airflow
Slots
Leak-tight Type 304 stainless steel
Curved stainless steel inlet grille
liner
Type 304 stainless steel dished Accessory Base Stand (sold
work surface with lift out knobs separately)

5. Purifier® Logic® Biosafety Cabinet
Being Green is being responsible
Labconco is a member of the United
States Green Building Council (USGBC).
Three Labconco product lines contribute to
LEED® points:
Purifier® Logic® Biosafety Cabinets
FlaskScrubber® and SteamScrubber® Laboratory
Glassware Washers
Protector® XStream® Laboratory Fume Hoods

6. Purifier® Logic® Motor
Intelligence Built In
New motor technology
• Electronically Commutated Motor (ECM)
• Over 3 million in use
Customer Advantages
• Quieter
• Cooler Operation
• Energy savings of 60%
• More reliable for correct airflow maintenance
• Continuously outputs information to be
displayed and triggers alarms when out of
parameters

7. Purifier® Logic® Biosafety Cabinet
Why is it cooler and quieter?
• The ECM is more energy
efficient ~ less energy is wasted
as heat

8. Purifier® Logic® Biosafety Cabinet
Intelligence Built In
How do we know it is running okay?
• The motor is programmed to maintain constant
airflow as the filter loads, no sensors needed
How does that work?
• Each BSC is tested at various airflows and
volumes
• Software uses data to generate a motor-blower
curve programmed into each motor
• The motor remembers the speeds and torques
required as filter pressures change

9. Purifier® Logic® Biosafety Cabinet
How do we know the ECM is running correctly?
• The ECM continuously outputs information to be displayed and
triggers an airflow alert when the motor speed changes suddenly
How do we know to change the filters?
• Motor initial and maximum speeds are calculated and filter life
remaining is figured

10. Purifier® Logic® Biosafety Cabinet
The conventional motor used in biosafety cabinets is a Permanent Split
Capacitor (PSC) motor. It is known as an induction motor.
Labconco Delta (pre-2008)
NuAire LabGard
ESCO Labculture
ECM PSC motor
Permanent magnet on the rotor Magnetic field must be induced
More efficient Generates a lot of waste heat
Performance feedback No feedback

11. Purifier® Logic® Biosafety Cabinet
How the Logic ECM is better
Heat is the #1 killer of motors because it reduces the life of the
bearing/bushing lubricant. Because the PSC runs hotter, it will fail faster than
an ECM.
The ECM uses hardened ball bearings on the rotor-most PSC motors use
simple bushings.
Cooler operation and bearings in the ECM means a much longer lifespan for
the ECM…rated to 50,000 hours.

12. Purifier® Logic® Biosafety Cabinet
Logic ECM energy comparison
The ECM saves significant energy compared to other motor
technologies used in biosafety cabinets.
Power Consumption (Watts)*
ECM PSC 3-Phase
4' BSC** 290 582 480
6' BSC** 490 1440 804
*At 120V
** 10" sash height

13. Purifier® Logic® Biosafety Cabinet
LCD Information Center
Line-of-sight on inside wall
Filter status
Cabinet status
Error messages
Icons indicate features on/off
Interval or “stop watch” timer
Fluorescent/UV Hour meter with alarm

14. Purifier® Logic® Biosafety Cabinet
LCD Information Center
Status area
• “OK”
• “Sash is too high”
• “Airflow Alert”
• “System Error”
Data area
• Displays the filter remaining,
inflow and downflow velocities
Icon Area
• Displays icons of active features

15. Purifier® Logic® Biosafety Cabinet
Icons
Normal blower operation
Night-Smart blower operation
Fluorescent light
UV light
Timer
Security Lock
Mute

16. Purifier® Logic® Biosafety Cabinet
Simple interior design
• Easy to clean
• Electrical duplex up and
out of work surface area
• Accessories keep work
area uncluttered
• Improved lighting
Dual fluorescent lamps
instead of one

17. Purifier® Logic® Biosafety Cabinet
Simple interior design
• No thumb screws
• Lifting knobs on both ends
• Work surface support to assist for
removal
• Towel catch/Pre-filter retained
Towel Catch
without fasteners
• Smooth work surface
Work Surface Support

18. Purifier Logic® Biosafety Cabinet
Sash Changes
• Viewing height increased 1.75"
• Angled sash with no sash handle
across the bottom edge for better
viewing
• Formed handles attached to sash
for easy gripping

19. Purifier® Logic® Biosafety Cabinet
Dimensions
Lower exterior height
• Approx. 3/4" lower overall
Greater interior height
• Approx. 2" taller in the front
Increased viewing height

20. Purifier® Logic® Accessories
Improved Filter Options
• Accept ULPA filters
• Optional pre-filters
• Optional carbon post-filters on 4' - 6' models
Stick-on Foam
Stick-
Prefilter
Carbon Filtered Exhaust

21. Purifier® Logic® Biosafety Cabinets
Accessories
Telescoping Base Stand
Adjusts in 1 inch increments from 27"
to 34.5"
NSF-listed and ADA-compliant
Durable epoxy coating
Available with fixed leveling feet or toe-
locking polyurethane casters
Seismic models available

22. Purifier® Logic® Biosafety Cabinets
Accessories
Hydraulic Lift Base Stand
Built-in electric or manual hydraulic lift
Height is adjustable from 25.5" to 33.5"
Supports loads up to 1000 pounds
Available in 3', 4', 5' and 6' widths
NSF-listed and ADA-compliant
Left: Manual Hydraulic Lift Base
Stand is shown with Caster Kit
installed.

23. Purifier® Logic® Biosafety Cabinets
Accessories
Optional Wire Shelves (stainless steel snap-in)
Optional Cord/Tubing management hooks

24. Purifier® Logic® Biosafety Cabinet
Accessories
Stainless Steel Turntable
Vacuum Aspiration Pump

25. Purifier® Logic® Biosafety Cabinet
Accessories
Airflow Sensor Kit (not shown)
• Kit allows for installation of airflow sensors in the field
• Optional sensor can be factory installed
• Recognizes sensor and displays inflow (after calibration)
IV Bar supports intravenous solution bottles and bags
UV Light Kits include one 254 nm UV lamp
Service Fixture

26. Purifier® Logic® Biosafety Cabinet
Accessories
Logic® Cart
Provides convenient supply storage
Fits easily under the Purifier Logic
Class II Safety Cabinets mounted on Base
Stand
2" casters provide mobility

27. Purifier® Logic® Biosafety Cabinet
Accessories
Ergonomic Chair
• 6-way articulating seat
• Removable armrests
Adjustable Footrest
• Elevate feet and permits angle
repositioning while in use

28. Purifier® Logic® Biosafety Cabinet
Accessories
Support Shelves
• Provide work surface to support remote
equipment
• Epoxy-coated steel shelf mounts to left-side
frame of base stand
Laptop Computer Arm
• Securely supports laptop computer
• Articulating arm raises, lowers and pivots to
a steady position

29. Purifier® Logic® Agency Approvals
NSF Standard 49 Listed
• With carbon post-filter and pre-filter
installed
• With accessory shelves, hooks, and IV bar
in place
ETL, ETL-C and CE mark
Accessory Base stand tip tested by NSF

30. Working in Biological Safety Cabinets
Planning
Thoroughly understand procedures and
equipment required before beginning work
and arrange for minimal disruptions
Have disinfectant and spill cleanup
materials prepared

31. Working in Biological Safety Cabinets
Start Up
Turn off UV light, open sash to its proper
height, and turn on cabinet lights and blower
Check grille for obstructions and let the
cabinet operate for 5 minutes
Wash hands and arms thoroughly with
disinfectant soap; wear a long sleeved lab
coat and gloves
Use eye protection

32. Working in Biological Safety Cabinets
Wipe-Down
Wipe down all interior surfaces of the
work area with a solution of 70%
ethanol or other suitable disinfectant

33. Working in Biological Safety Cabinets
Loading
Load only the materials needed. Do not
overload the cabinet or obstruct the
grille. Keep large objects separated.
Lower the sash until it is in its proper
position. Allow the unit to operate for
2 to 3 minutes to purge any airborne
contaminants.

34. Working in Biological Safety Cabinets
Work Techniques
Keep materials at least 4" inside the sash opening
and perform all contaminated operations as far to
the rear of the work area as possible.
Segregate clean and contaminated materials.
Arrange materials to minimize movement of
contaminated materials into clean areas. Keep all
contaminated material in the rear of the work area.
Avoid excessive movement of arms or materials
through the front opening during operation.

35. Working in Biological Safety Cabinets
Work Techniques
Use proper aseptic technique.
Avoid techniques that disrupt airflow patterns in
the cabinet, such as an open flame.
If there is a spill or splatter during use, all objects
must be decontaminated before removal. Thoroughly
disinfect the interior surfaces of the cabinet while it is
still in operation.

36. Working in Biological Safety Cabinets
Final Purging
After completing work, allow the cabinet
to operate for 2 to 3 minutes undisturbed
to purge airborne contaminants from the
work area.

37. Working in Biological Safety Cabinets
Wipe-Down
Periodically lift the work surface and
clean underneath it. Clean the towel catch.
Dispose of rubber gloves and have lab
coat properly laundered. Wash arms and
hands thoroughly with germicidal soap.
Wipe down all interior surfaces of the
work area with a suitable disinfectant.

38. Working in Biological Safety Cabinets
Shutdown
Turn off the fluorescent light and
cabinet blower, close the sash and turn
on the UV light if appropriate.

39. Cabinet Maintenance
User Performed Maintenance
Replace fluorescent and UV lamps annually
Reset circuit breakers
Airflow smoke test
Establish a smoke split
• Mark work surface where smoke split occurs
• May use smoke stick or dry ice to determine
where split occurs
• User should work behind smoke split

40. Cabinet Maintenance
Certification
A validation of cabinet integrity and
performance
Performed by a qualified
independent technician
Performed when the cabinet is newly
installed, annually thereafter, or if the
cabinet is moved to a new location

41. Cabinet Maintenance
Certification Tests
HEPA filter leak test
Inflow and downflow verification
Airflow smoke pattern test
Electrical safety
Vibration, sound and lighting intensity
tests (optional comfort tests)

42. Cabinet Maintenance
What to Look For in a Certifier
Accredited by an independent organization
(NSF, AFCA, CETA)
Has attended cabinet manufacturer certifier
training course
Membership in professional organizations
Experience with the cabinet type and model
References
Written estimates before work is begun

43. Operating Procedures
Myths, Lies and Gobbledygook
"I've got to use a Bunsen burner in my Biosafety Cabinet..."
During operation, the flame of a burner is very disruptive to the airflow patterns of the cabinet, and may
actually increase the dispersion of aerosols throughout the work area.
If the flame of the burner is too large, the excessive heat may melt the adhesive
holding the HEPA filter together or literally burn holes in the filter media. An
unattended burner may blow out. In a Type A1 or A2 cabinet, the gas may reach
explosive concentrations.
Use alternate methods, such as electric incinerators, or disposable inoculating loops.
Don’t flame bottle mouths; the work area of the biosafety cabinet should be a sterile
environment, if used properly.

44. Operating Procedures
Myths, Lies and Gobbledygook
"I can use a biological safety cabinet as if it were a fume hood..."
Do NOT use a biosafety cabinet like a fume hood!
The Fume Hood is designed to remove noxious or toxic fumes and aerosols away from the
operator. It should be constructed of materials that are inert to a wide variety of chemical agents.
The Biosafety Cabinet's primary purpose is to protect the operator, environment, and often the
product from biohazardous contaminants. The biosafety cabinet and its HEPA filters are constructed
of materials that are inert to the chemicals used in connection with biological research, but may be
damaged by some of the more corrosive chemicals commonly used in fume hoods.

45. Operating Procedures
Myths, Lies and Gobbledygook
"If I work in a biohazard cabinet, I don't have
to be as careful with my technique."
Wrong. The biosafety cabinet will provide Personnel and
Product protection only if used properly. Aseptic technique
must be practiced at all times while working in a Biohazard
Cabinet.

46. Operating Procedures
Myths, Lies and Gobbledygook
"If I use the UV light, do I need to decontaminate the work area?"
Yes. The UV light is only an adjunct, to minimize
contamination of the work area when the cabinet
is not in use. UV light has practically no
penetrating power, and will not kill microbes
protected by dust, dirt, or organic material.
The best method to prevent contamination in the
cabinet is regular decontamination of the work
area surfaces, before and after each work
session.

47. Operating Procedures
Myths, Lies and Gobbledygook
"Can I put a centrifuge in the biosafety cabinet? "
You shouldn’t. Large objects placed in the work area will impede the
airflow, reducing the efficiency of the cabinet. Electrical appliances
like centrifuges, blenders, etc. often have cooling fans that can
disrupt the cabinet’s airflow. It’s better to use sealed safety cups in
the centrifuge that are loaded and unloaded in the safety cabinet.

48. Operating Procedures
Myths, Lies and Gobbledygook
"A Type B cabinet will give better protection than a Type A."
Not Necessarily. If you’re talking about protection from
volatile toxic chemicals, yes; if your talking about
protection from biohazardous aerosols, no.
Assuming the units being considered are both NSF
listed, they were both subjected to the same biological
challenge testing. Any claims of superior containment
should be documented.

49. Operating Procedures
Myths, Lies and Gobbledygook
"All biohazard cabinets should operate continuously.”
In some applications, such as working with cytotoxic drugs, it is
recommended the cabinet operate continuously, to prevent the escape of
toxic material.
In most applications, operating the cabinet continuously will only shorten
the life of the HEPA filters.

50. Operating Procedures
Myths, Lies and Gobbledygook
"If I leave the biosafety cabinet operating
continuously, it will clean the air to Class 5
conditions.”
Assuming you have an air-tight room, with no ventilation system,
and no activity in it, then a recirculating Type A cabinet might
clean the air to Class 5 levels. Unfortunately, as soon as the user
entered the room, particle-laden air would re-contaminate the
room.
All this will really do is shorten the life of the HEPA filters.

51. Operating Procedures
Myths, Lies and Gobbledygook
"I’ve heard that you should replace HEPA filters annually.”
Not necessarily, HEPA filters should be replaced when the pressure differential
across them becomes excessive. The Purifier Logic Filter Life gauge will alert the
user when the HEPA filters should be replaced.
The two biggest factors in filter life are the level of contaminants in the room air,
and how many hours the cabinet operates.
Arbitrarily replacing HEPA filters on a timetable, without regard to loading may be
unnecessarily expensive and wasteful.

52. Labconco Corporation
8811 Prospect Ave.
Kansas City, MO 64132
1-800-821-5525
816-333-8811
www.labconco.com

53. www.labconco.com