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Babol7-3-15
Histology
Doc. Alma Maria Daguay M.D.
Spermatogenesis and
Spermiogenesis
 The process whereby haploid gametes (reproductive sex
cells) are created
 Spermatogenesis in males
 Oogenesis in females
Gametogenesis
 Begins at puberty
 Proliferation of stem and progenitor cells called
spermatogonia
 Dark ovoid nuclei
 Act as stem cell and divide to form new stem cells
Spermatogenesis
1. Type A spermatogonia: clonal divisions and interconnected as
a syncytium
2. Type B spermatogonia: spherical and pale nuclei
3. Each type B produce two cells called primary spermatocytes
4. First meiotic division:produce smaller cells called secondary
spermatocyte
5. Secondary spermatocyte separates the chromatids;produce
two haploid cells called spermatids
6. Spermatids undergo morphological
differentiation;spermatozoa
Summary of Spermatogenesis
ABPSSS
SG
PS
M
F
SC
ES
LS
SS
LC
 Cell differentiation in which spermatids become sperm
 Flattening of nucleus
 Formation of acrosome
 Growth of tail
 Reorganization of mitochondria
 Shedding of unnecessary cytoplasm
Spermiogenesis
 Proacrosomal vesicles coalesce as a single acrosomal cap
 Centrioles migrate and act as basal body
Golgi Phase
 Acrosomal cap spreads over half of the nucleus
 Contain acrosin
Cap phase
 Head remains embedded in the sertoli cell
 Growing axoneme extends to the tubule
 Nuclei become elongated and condensed
 Mitochondria migrate through actin filaments called
manchette to form the middle piece
Acrosome phase
 Unneeded cytoplasm is shed as residual body
 Intercellular bridges are lost
Maturation phase
 Establishing blood testis barrier
Sertoli cells
An update on sperm retrieval techniques
for azoospermic males
Non ejaculated sperm is aspirated from the
epididymis and testis of the patient using
surgical method
Update
 Open Epididymal Fine-Needle Aspiration
 Testicular Sperm Extraction (TESE)
 Single Seminiferous Tubule Biopsy
 Percutaneous Epididymal Sperm Aspiration (PESA)
 Testicular Sperm Aspiration (TESA) and Testicular Fine-
Needle Aspiration (TEFNA)
 Microsurgical Epididymal Sperm Aspiration (MESA)

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Spermatogenesis and spermiogenesis

  • 1. Babol7-3-15 Histology Doc. Alma Maria Daguay M.D. Spermatogenesis and Spermiogenesis
  • 2.  The process whereby haploid gametes (reproductive sex cells) are created  Spermatogenesis in males  Oogenesis in females Gametogenesis
  • 3.  Begins at puberty  Proliferation of stem and progenitor cells called spermatogonia  Dark ovoid nuclei  Act as stem cell and divide to form new stem cells Spermatogenesis
  • 4. 1. Type A spermatogonia: clonal divisions and interconnected as a syncytium 2. Type B spermatogonia: spherical and pale nuclei 3. Each type B produce two cells called primary spermatocytes 4. First meiotic division:produce smaller cells called secondary spermatocyte 5. Secondary spermatocyte separates the chromatids;produce two haploid cells called spermatids 6. Spermatids undergo morphological differentiation;spermatozoa Summary of Spermatogenesis ABPSSS
  • 5.
  • 7.  Cell differentiation in which spermatids become sperm  Flattening of nucleus  Formation of acrosome  Growth of tail  Reorganization of mitochondria  Shedding of unnecessary cytoplasm Spermiogenesis
  • 8.  Proacrosomal vesicles coalesce as a single acrosomal cap  Centrioles migrate and act as basal body Golgi Phase
  • 9.  Acrosomal cap spreads over half of the nucleus  Contain acrosin Cap phase
  • 10.  Head remains embedded in the sertoli cell  Growing axoneme extends to the tubule  Nuclei become elongated and condensed  Mitochondria migrate through actin filaments called manchette to form the middle piece Acrosome phase
  • 11.  Unneeded cytoplasm is shed as residual body  Intercellular bridges are lost Maturation phase
  • 12.
  • 13.
  • 14.  Establishing blood testis barrier Sertoli cells
  • 15. An update on sperm retrieval techniques for azoospermic males Non ejaculated sperm is aspirated from the epididymis and testis of the patient using surgical method Update
  • 16.  Open Epididymal Fine-Needle Aspiration  Testicular Sperm Extraction (TESE)  Single Seminiferous Tubule Biopsy  Percutaneous Epididymal Sperm Aspiration (PESA)  Testicular Sperm Aspiration (TESA) and Testicular Fine- Needle Aspiration (TEFNA)  Microsurgical Epididymal Sperm Aspiration (MESA)