The document provides information about an upcoming ASE Symposium on August 16th where students will present their summer research accomplishments through oral presentations and posters. It outlines the schedule of events, which includes introductions, presentations, lunch, and a networking session. Students are given tips and guidelines for preparing their oral presentations, posters, summary reports, and thank you letters to share at the symposium. They are encouraged to practice their talks and rehearse their "pitch" for the poster session. Support resources are also listed to help students succeed at the symposium.

1. Showcase your accomplishments!
Stacey Johnson, ASETeacher Monitor
Preparing for the ASE Symposium,
Friday, August 16th at UP

2. prepare * practice * prepare * practice
What Happens AtThe Symposium?
• Introductions and Keynote Speaker
• Oral Presentations
• Lunch and Recognitions
• Poster Presentations
• Networking

3. 2019 ASE Symposium

4. • Dress
• Oral Presentations
• SymposiumTitle &
Registration
• Poster
• ASE Summary
• ThankYou Letter
saturdayacademy.org/ase-symposium
What’s coming up?

5. Oral Presentations
• Sessions have 3-4 presentations
• 10-30 attendees per session
• Each talk is 12 minutes
• Plus 3 minutes for questions
• Joint talks allowed (doubles time)
https://www.youtube.com/watch?v=S5c1susCPAE

6. • Model a speaker you like
• Pose a question or tell a story
• Use simple terms & clean design
• Think about your audience
CyaberAcademy: ContentPreparing & Giving your Presentation

7. CyaberAcademy: Content
https://www.ted.com/talks/camille_seaman_photos_from_a_storm_chaser
Presentation Style
Enjoyable?
Feedback?
Questions?

8. Thinking about Good Presentations
• Prepare an outline
• Use story, build background
• Think about your title

9. • “We’ve GotYour Rack: Discovering Engineering at
Yakima Products Inc.”
• “Risky Business”
• “An Analysis of the Conductivity of Encap Polymer
AdhesivesThrough Impedance Spectroscopy”
• “Recovery and Digitization of HistoricTide Data“
Register online by July 22nd
SymposiumTitle & Registration: examples

10. CyaberAcademy: Content
saturdayacademy.org/current-interns
MakingYour Poster – Details
• Follow ASE poster rules
• Check out the handbook…
“Preparing for the ASE Symposium”

11. CyaberAcademy: ContentMakingYour Poster - Displays
• Two posters will
hang on a stand
with binder clips.
• Do NOT use
cardboard or foam
core board
 3 ft   3 ft 

12. CyaberAcademy: ContentExample Format
 Start Now!

13. CyaberAcademy: ContentPoster Activity
• Look at the following posters
• Focus on first impressions
 What do you like about these posters?
 What is distracting or hard to read about
these posters?

14. Screening Synthetic Analogs of Pactamycin for Anti-Cancer
Properties in Melanoma
Asha Pantula1, Evan Carpenter2, Monet Miller2, Gitali Ganguli-Indra2,3,4, Arup Indra2,3,4,5
Background
Materials
Results
Future Developments
Acknowledgments
• Melanoma: One of the most
dangerous and treatment
resistant human cancers (1).
Before treatment 15 weeks of Vemurafenib (treatment) 32 weeks of
Vemurafenib
Need: To find a potent yet still
efficient therapy(2).
• Pactamycin: a natural
compound with many
anticancer properties and
high cytotoxicity levels (4).
• Synthetic analogs have been
produced, hoping to lower
toxicity levels (3,4).
 Worked with A375 line of human melanoma cells
 Added analogs of Pactamycin and Vehicle (DMSO)
1. TM026 is a positive control
2. TM117 and TM118: different analogs of Pactamycin
 Screened compounds through high throughput characterization
Added cell titer glow reagent which measures ATP
(cell energy) and gives ATP count
 Bright-field microscopy to identify impact on morphology
 Immunocytochemistry to observe impact on proliferation
• Analogs showing anti-
cancer properties (TM026,
TM122, and TM123) will be
tested on normal human
melanocytes, to test toxicity
levels.
• There will also be new
research on the mechanism
of action of these
compounds as well as the
morphology of the cells.
• TM026 showed strong anti-
cancer properties against
melanoma like previously
shown in head and neck
squamous cell carcinoma
cells
• TM122, and TM123 showed
strong anti-cancer traits
against metastatic
melanoma.
• TM117, TM118, TM119,
TM120, TM121, TM124, and
TM125 analogs had no
impact on the growth of the
metastatic melanoma,
A375 line of Melanoma
containing BRAF (V600E)
mutation, analogs of Pactamycin,
Growth Media (DMEM),Vehicle
(DMSO), Cell Titer Glo Assay kit
Methods
Figure A: Graph shows
that TM026 has an IC-50
value of 3.045 uM . Neither
TM117 nor TM118 showed
any anti-cancer activity in
A375 cell line.
I would like to thank Saturday
Academy for the opportunity, the
College of Pharmacy at OSU,
and all the members of the Indra
lab for their help and support.
Objective
Figure B: TM122, TM123
and TM026 all showed
anti-cancer activity against
melanoma cells.
References
1Nikolaou, Vasiliki A. et al. Melanoma: New Insights and New Therapies." J Invest Dermatol.
2012 Mar;132(3 Pt 2):854-63.
2Wagle, Nikhil, et al. "Dissecting Therapeutic Resistance to RAF Inhibition in Melanoma by
Tumor Genomic Profiling." Journal of Clinical Onocology 29.22 (2011): 3085-096.
3Lu W, Roongsawang N, Mahmud T. Biosynthetic Studies and Genetic Engineering of
Pactamycin Analogs with Improved Selectivity toward Malarial Parasites. Chem Biol.
2011;18: 425–431. pmid:21513878
.
1Saturday Academy, 2College of Pharmacy, 3Linus Pauling Institute, Oregon State University; Corvallis, OR
4Dept. of Dermatology, 5Knight Cancer Institute, Oregon Health & Sciences University; Portland, OR
Conclusions
Figure C: Bright-field microscopy shows compound TM026’s
(right) ability to kill melanoma cells thereby reducing cell density,
and reduce cell size and shape.
Figure D: Immunocytochemistry using anti-Ki67 antibody exhibits
reduced proliferation of TM026 treated melanoma cells compared
to the vehicle control.
A.
B.
E.
Figure E: Graph shows less cells treated
with TM026 inhibit Ki67 (marker for
proliferation)
Effect of TM026 on
Proliferation
- 4 - 3 - 2 - 1 0 1 2
0
2 5
5 0
7 5
1 0 0
1 2 5
E f f e c t o f P a c t a m y c i n A n a l o g s o n A 3 7 5 l i n e
L o g D o s e C o n c e n t r a t i o n
%CellSurvival
T M 0 2 6
T M 1 1 7
T M 1 1 8
P a c t a m y c i n A n a l o g u e D o s e R e s p o n s e C u r v e i n A 3 7 5
l o g [ C o m p o u n d ] (  M )
%CellSurvival
- 4 - 3 - 2 - 1 0 1 2
0
2 5
5 0
7 5
1 0 0
1 2 5
T M 0 2 6
T M 1 1 7
T M 1 1 8
T M 1 1 9
T M 1 2 0
T M 1 2 1
T M 1 2 2
T M 1 2 3
T M 1 2 4
T M 1 2 5
U.S. Melanoma Incidence*. Digital image. Planning Cancer.
N.p., n.d. Web.
To determine if synthetic analogs of
Pactamycin have anti-cancer
properties and less toxicity levels
against human metastatic melanoma.
Poster 1:

15. CyaberAcademy: Content
• Symposium prep = HOMEWORK
• Review poster and presentation with mentor(s)
• Practice your talk and timing
• Practice some more…
• …then, practice again
• Rehearse but don’t recite
.
Tips for Presentation Success
Poster 2:

16. CyaberAcademy: Content
• Stand to the side of poster – not in front
• Remember the tips from the presentation video
• Remain at your poster entire session
• Prepare your “pitch” ahead of time…
Tips for Poster Session

17. CyaberAcademy: ContentSupport for Symposium Success
• ASE Symposium Handbook
• Teacher Monitor
• Mentors

18. CyaberAcademy: ContentSummary Report
In your words…
• Your work and contributions over
the summer.
• Likely to share content with
poster and talk.
Submit Online Wed., Aug. 21st

19. CyaberAcademy: ContentThankYou Letters: Expressing Gratitude
Personalized & Specific
“ Your patience and sense of humor made the
long hours seem short”
Sincere & Heartfelt
“Seeing you and other scientists enthusiastic
about science has inspired my own interest.”
Gratitude & Appreciation
“I appreciate you giving me this amazing
opportunity and mentoring me!”

21.  Discuss title and content with mentor ASAP
 Register by Mon., Jul. 22nd
 ASE will email you a list of thank you letter
recipients by end of July
 Email your letters to yourTeacher Monitor by
Friday., Aug. 9th
 Bring signedThankYou Letters to Symposium on
Fri., Aug. 16th
 Submit Summary by Wed., Aug. 21st
Next Steps

22. … follow your curiosity!
Questions???