1. Induced breeding of Tinfoil Barb
(Barbonymus schwanenfeldii) (Bleeker, 1854)
using Ovaprim .
By
E.D.M. Epasinghe, A.M.A.N. Adikari, T.A.D.W. Karunaratne,
H.M.P. Kithsiri, V. Pahalawattarachchi
2. Tinfoil Barb [TFB]
§ Globally popular aquarium Cyprinid
§ Native to Southeast Asia
§ No authentic records on its natural
spawning in captive condition in Sri
Lanka
Objective of breeding TFB
§ Inducing the spawning of captive reared TFB using
Ovaprim
§ Introducing less time consuming and fruitful breeding
method for commercial scale fish breeders in Sri Lanka
3. Materials and methods
Inducing agent - Ovaprim
— Uses to induce ovulation and
spermiation in fish
— A synthetic GnRH [1 mL
contains 20 µg of GnRH + 10
mg Domperidon]
— Ready to inject product
liquid
4. Selection of brood fish
— Selected randomly based on
their external features.
— Distended abdominal region-
— Normal abdominal region-
— Sedated fish in 65 mg/L TMS
— Females were subjected to Intra
Ovarian Biopsy.
— Males were checked for
secretion of milt
Materials and methods
5. Confirmation of fish maturity,
— 18 females with Migratory stage GV
— 18 males Trickled milt when a slight
pressure was applied at the vent
Administration of Ovaprim within
minimum possible period of time
— Injected single dose
— IM injection to the dorsal musculature
Materials and methods
Avg: BW
0.23 ± 0.01
kg
7. Dose
mL/kg of
BW
0.20 0.30 0.40 0.50 0.60 Control
Un injected
0.10 0.15 0.20 0.25 0.30 Un injected
The injected TFBs were kept at 100 L glass tank with preconditioned
water aerating water continuously
Sex ration 1:1 male to female
Each experimental group comprised of three replicates.
Materials and methods
Hormone doses
Breeding
performance
Ovulation time (hrs)
Fertility rate (%)
In addition,
data
collected on
Post experimental mortality rate of brooders
Water quality parameters in the experimental tanks
8. Identification of Ovulation time
— In order to identify the correct ovulating time - after 3
hrs by the injection
— Females vent was slightly pressed very carefully to facilitate
come out eggs- repeated in every half an hour
— Did not try by force
— Expected free flow of eggs after completion of ovulation
— Collecting of milt
— Just after confirmed the complete ovulation in females
— Males were stripped first
— Fertilization
— Dry method was followed to fertilize eggs
— Fertility rate
— Sub sample of eggs checked under the microscope to identify the
first cleavage stage of embryonic development.
9. Cleavage stages of embryonic development of TFB
4 Cell
16 Cell
Cleavage
Blastula
10. Results and discussion.
Dose
mL/kg
of BW
0.20 0.30 0.40 0.50 0.60 Control
Un injected
0.10 0.15 0.20 0.25 0.30
Ovulation
time/ hrs
11.00 ±
0.51a
9.20 ±
0.17b
5.43 ±
0.51c
6.10
± 0.17c
3.20 ±
0.17d
not
observed
Fertility
rate %
27.84
± 2.36a
50.5 ±
7.09b
73.33 ±
4.51c
80.67
± 5.13c
33.33 ±
9.07a
not
observed
Data was analyzed with one way ANOVA and the Tukey test in SPSS software.
Values are presented as means ± S.D.
Means in each raw with different superscripts are significantly different from
each other.
13. Results and discussion.
— In the post spawning period
— survival rate of brooders was 100%
— the range of hormone dosages - not harmful
— During the latency period temperature ranged from
27.4 0C 27.8 0C , pH was measured as 7.3, Breeding
tanks were aerated continuosly
14. Conclusion
— Recommended dosages of obtain highest fertility rates
and relatively short ovulation times are 0.4 -0.5 mlkg-1
single dosage of Ovaprim to the female and half the
dosage to the male at a same time.
Female fish should be induced when they are at the
peak of GV migratory stage (stage IV)
and,
Male should ooze sperm with gentle pressure.
15. Dose calculation
— This is a 1.0 mL cyringe.
— It has been graduated in to the 50 units.
— Therefore, a one unit is equal to 0.02 mL
— We need to adjust 1 unit = 0.005 mL of hormone
Normal saline 0.75 ml 0.25 ml
hormone
Dose
mL/kg of
BW
0.2 0.3 0.4 0.5
Recommended dose
by producer
0.6 Control
Un injected
0.1 0.15 0.2 0.25 0.3 Un injected
For 0.23 kgFor 0.23 kg
of BW /unitof BW /unit
9 14 18 23 28
4.5 7 9 12 14
16. To improve hatchability
— Introduce different hatching techniques
in order to increase the hatching rate
— Eggs are semi buoyant
Future research needs
Effect of other hormone products which contains GnRH
on TFB breeding should be monitored
WOVA.FH , Ovatide®, Ovulin® - synthetic products
![Tinfoil Barb [TFB]
§ Globally popular aquarium Cyprinid
§ Native to Southeast Asia
§ No authentic records on its natural
spawning in captive condition in Sri
Lanka
Objective of breeding TFB
§ Inducing the spawning of captive reared TFB using
Ovaprim
§ Introducing less time consuming and fruitful breeding
method for commercial scale fish breeders in Sri Lanka](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)