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CHAPTER 4
ACTIVITY 4,2 Plant transport systems
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Prelab activity (key concepts)
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vCE Units 7&2 Student Activv Manual A 2006
$ ithout the permission of the publisher
Nelson Australia Pty Ltd

2. t
lntrod uction
Vater is an essential requiremenr for photos'nthesis
ro occur. As photosynthesis mostly occurs ,
Ieaves of plants, warer and subsrances dissohled in the
in it, move ,h.o.rgi vascular plants in the xylem,
the roots to the lea'es' Xriem consists ofhollorv from a
cells oftracheids and vessels. Sugars,
in the form ofthe
disaccharide sucrose' and other nurrients move
in the phloem. The phloem is made up of sieve a
companion cells and
cells.
Pu rpose
To examine ho*'*'arer is transported through
a celery stalk and leaves.
Materials
Each group rvill require:
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fJ a srick ofcelery that has been standing in red food dye
or eosin for several hours, cut2 cmfrom its
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base
[l single-edged razor blade e
fl microscope, srereo microscope or hand iens
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fl microscope slides
fl mounted needles (optional)
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fl millimetre ruler' transverse and longitudinal stem
se-ions of Helianthu.r (for Extension Activities).
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Procedure e
fl R.rnou" rhe celery from the coloured solution.
U Examine the stalk and leaves for spread of the
dye; holding them up to the light may help.
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the areas where the dye is concentrated. Draw observe
'Observations' below.
the distribuiion of die in ,n. rlri" eigure
4.2 under e
fl cut thin (t mm) transverse sections across the stem and branch (and leaf e
if possible). Arrange the
sections onto a microscope slide.
D E*amine the sections ,,,,d.. loru po'er and make
diagrams shorving the distribution of the
dye.
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D C.,t anorher fresh 1-2 mm piece of stem.
fl utt the razor blade to cut out a small piece of
rhe area coloured by the dye and put this
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on a
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microscope slide.
ur. a couple of razor brades or mounted needles ro rease the piece 4
of tissue apart.
E aaa a coverslip, using a drop of water if the tissue
is becoming dry. I
c ur. low power, th.., high po-., to find 'spirai' or 'coils,. Drari,
th"se in Figure 4.3.
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Ohservations +
Figure 4.2 Distribution of dye in celery leaf. e
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Nelson BioloEt/ vcE lJnits 1&2 student Activity
Manuat @ 2006 Nerson Austraria pty Ltd.
Not to be reproduced without the permission
of the publLher.
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3. Figure 4.3 Spirals/coils found in vascular tissue of celery.
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c The spirals have functions equivalent to those of the spirals of cartilage around the trachea.
7hat are their functions? €
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Conclusion e
Refer back to the purpose of this activin'- Have you achieved this purpose? Write a conclusion based on
what you have learnt by undertaking rhis activiry. e
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