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E coli, Klebsiella  and  Enterobacter Prof M.I.N. Matee School of Medicine MUCHS
Escherichia coli ,[object Object],[object Object],[object Object]
Morphology and culture characteristics ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Antigenic structure ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
E. coli  toxins ,[object Object],[object Object],[object Object],[object Object],[object Object]
E. coli  toxins ,[object Object]
Urinary tract infections ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object]
Enterotoxigenic  E. coli   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
Escherichia coli O157:H7 ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Treatment of  E.coli  related diarrhoea   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Pneumonia ,[object Object],[object Object],[object Object]
Klebsiella spp
Morphology and culture characteristics ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
K pneumoniae   and  K oxytoca   Hospital acquired infections due to  K rhinoscleromatis  produces rhinoscleroma, condition with destructive granuloma of the nose and pharynx . K ozenae – progressive atrophy of nasal mucous membrane
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object]
Diagnosis of Bacterial Infection culture on plates or in broth  identification by biochemical or serological tests on pure growth from single colony microscopy Decolorise  Counterstain Stain unstained or stained with e.g. Gram stain   sensitivities Serodiagnosis DNA technologies by disc diffusion methods, breakpoints or MICs
Gram negative rods
Lactose fermentor on MCA
Lactose positive  Klebsiella  sp
E. coli
 
Indole production ,[object Object],[object Object],Negative Positive
[object Object],[object Object]
A=Acidic K=Alkaline - - + - - GAS  + + - - - HYDROGEN SULFIDE  A A A A - BUTT  K K A A - SLANT  TUBE 5  TUBE 4  TUBE 3  TUBE 2  TUBE 1 (UNINOCULATED) INTERPRETATION OF TUBES ABOVE
ONPG test ,[object Object],[object Object],[object Object],Positive  Negative
Methyl Red ,[object Object],[object Object],[object Object]
Voges-Proskauer ,[object Object],[object Object],[object Object],[object Object]
MR/VP continued ,[object Object],[object Object],[object Object],Methyl Red:  left – and right + VP:  left + and right –
IMViC test ,[object Object],[object Object],[object Object],[object Object],[object Object]
Indole (IMViC tests) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Methyl Red (MR) (IMViC tests) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Voges – Proskauer (VP) (IMViC tests) ,[object Object],[object Object],[object Object],[object Object],[object Object]
Citrate utilization ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Positive  Negative
Treatment of enteric bacteria ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
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E coli, klebsiella, enterobacter lecture notes

  • 1. E coli, Klebsiella and Enterobacter Prof M.I.N. Matee School of Medicine MUCHS
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
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  • 9.
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  • 20.
  • 21.
  • 22.
  • 23.
  • 25.
  • 26.
  • 27. K pneumoniae and K oxytoca Hospital acquired infections due to K rhinoscleromatis produces rhinoscleroma, condition with destructive granuloma of the nose and pharynx . K ozenae – progressive atrophy of nasal mucous membrane
  • 28.
  • 29.
  • 30.
  • 31. Diagnosis of Bacterial Infection culture on plates or in broth identification by biochemical or serological tests on pure growth from single colony microscopy Decolorise Counterstain Stain unstained or stained with e.g. Gram stain sensitivities Serodiagnosis DNA technologies by disc diffusion methods, breakpoints or MICs
  • 34. Lactose positive Klebsiella sp
  • 36.  
  • 37.
  • 38.
  • 39. A=Acidic K=Alkaline - - + - - GAS + + - - - HYDROGEN SULFIDE A A A A - BUTT K K A A - SLANT TUBE 5 TUBE 4 TUBE 3 TUBE 2 TUBE 1 (UNINOCULATED) INTERPRETATION OF TUBES ABOVE
  • 40.
  • 41.
  • 42.
  • 43.
  • 44.
  • 45.
  • 46.
  • 47.
  • 48.
  • 49.
  • 50.  
  • 51.  

Notes de l'éditeur

  1. EMB is both selective and differential