Creating and Analyzing Definitive Screening Designs
Phylum sporozoa or acomplexa
1. Phylum Sporozoa or Acomplexa
Classification
General Characteristics:
Obligate, intracellular protozoa
Distinct from other protozoa because they lack motor
organelles (cilia and flagella), except for the male gametes
during the sexual phase
Complicated life cycle, including sexual and asexual stages
Sexual- Sporogony/ Sporogonic Cycle
Asexual- Schizogony/ Schizogonic Cycle
They undergo schizogony/merogony (multiple fission/budding-production of merozoites),
gametogony (production of male and female gametes), and sporogony ( sexual stage-
production of sporozoites)
The sporozoites have apical complex which is specific to sporozoans, this structure is used for
attachment to the host.
Parasitic Species:
a. Sarcocystis species
Characteristics:
Includes the species Sarcocystis hominis and Sarcocystis suihominis
Causes sarcocystosis (muscular and intestinal) in humans
Hosts:
S. hominis
Intermediate host: cattle
Definitive host: humans
S. suihominis
Intermediate host: pigs
Definitive host: humans
Acquired through ingestion of infected uncooked / undercooked meat
Occurs worldwide but is common in areas where live stocks are raise
Most cases are reported in Asian tropics and subtropics
The prevalence of intestinal sarcocystosis is apparently low and is rarely associated with
illness
Phylum
Sporozoa/
Apicomplexia
Class Coccidia
Order
Eucoccidiida
Suborder
Eimeriina
Genus
Sarcocystis
Genus
Toxoplasma
Suborder
Haemosporina
Genus
Plasmodium
Subclass
Piroplasmea
Order
Piroplamida
Genus Babesia
2. Life Cycle:
The cysts with bradyzoites (infectice stage) ingested by humans will rupture in their intestines
releasing the bradyzoites, these bradyzoites will differentiate into microgamete and macrogametes.
The microgametes will fertilize the macrogametes resulting to oocysts. These oocysts will then be
shed from the host in the feces. If these oocysts will be ingested by the intermediate hosts ( pigs and
cattle) it will rupture in their blood vessels releasing sporozoites. These sporozoites will invade the
muscle tissues of the intermediate hosts and develops into cysts containing bradyzoites which will
then be passed into humans once ingested.
Symptoms:
1. Muscular sarcocystosis
Myositis
Dyspnea
Wheezing associated with eosinophilia
2. Intestinal sarcosystosis
Nausea
Stomach pain
Diarrhea
Diagnosis:
Fecal flotation method
IFA and ELISA
Treatment:
Albendazole
Metronidazole
Cotrimoxazole
Corticosteroid
b. Toxoplasma gondii
Characteristics:
Hosts:
Definitive hosts: domestic cat and other felines
Intermediate hosts: humans and mammals
Causes toxoplasmosis
Epidemiology:
Transmission: food borne, zoonotic, transplacental, organ transplant
95% of the world has been infected with toxoplosama
Infection is often highest in areas of the world that have hot, humid climates and lower
altitudes.
3. Life Cycle:
Sporocysts are shed from the feces of cats. These sporocsyts will undergo sporolation in the
environment which will contaminate foods and water. If contaminated foods and/or water in ingested
by livestock and humans it will undergo schizogony resulting into tachyzoites. Tachyzoites will invade
the different organs of the body including the brain and will develop into bradyzoites (cysts).
Tachyzoites can be transferred to another person through blood transfusion
Tachyzoits can overcome the placental barrier in pregnant women and develop into
bradyzoites (cysts) in the fetus’ brain.
Symptoms:
Immunocompetent – asymptomatic, (acute) cervical lymphadenopathy or flu-like illness
Immunodeficeint- retinochoroiditis, pneumonitis, or other systemic disease.
Patients with AIDS- intracerebral mass lessions
Congenital infection can result in abortion, still birth, encephalitis, chorioretinitis, and
hepatosplenomegaly
Diagnosis:
Observation of parasites in patient specimens, such as bronchoalveolar lavage material from
immunocompromised patients, or lymph node biopsy.
Isolation of parasites from blood or other body fluids, by intraperitoneal inoculation into mice or
tissue culture.
Detection of parasite genetic material by PCR, especially in detecting congenital infections in
utero.
Serologic testing is the routine method of diagnosis.
c. Plasmodium species
Characteristics:
Malaria is the dominant protozoan disease
From the Italian "mal' aria," meaning "bad air
All are transmitted primarily by the female Anopheles mosquito; also shared needles, blood
transfusions, and mother to child
Hosts:
Definitive host- Anopheles mosquito
Intermediate host- Human
500 million new cases/year (most in Africa)
Most frequent victims are children and young adults; ~ 2 million die/year
Species involved:
4. P. falciparum
P. Vivax
P. malariae
P. ovale
Life Cycle:
Exo-eryhtrocytic cycle (human)- when an infected anopheles mosquite bits a human,
sporozoite (infective stage) is injected into the host. The sporozoite will then invade the host’s
liver cells and undergo shizogony (multiple fission) which forms shizonts in the hepatic cells,
once the schizonts rupture it will release merozoites. These merozoites invade the red blood
cells. Others reinvade the liver cells resulting into another exo-erythrocytic cyle, some of them
remain dormant in the liver cells (hypnozoites) which will become active later on.
Erythrocytic cycle (human)- the merozoites that invade the RBC’s will form trophozoit/ring
stage (feeding stage- they feed on the hemoglobin of the RBC’s) and will undergo another
schizogony (multiple fission) which forms schizonts, if this schizonts will rupture (associated
with paroxysm experienced by the patient) it will release merozoites which will reinvade the red
blood cells initiating another erythrocytic cycle.
Gametogony- some of the trophoziotes will form gametocysts which will differentiate
into microgamete and macrogamete. These undergo no further development until taken by the
mosquito.
Sporogonic Cycle (mosquito)- If another female Anopheles mosquito bites an infected person it
sucks blood containing the different stages of malarial parasites. All stages other than the
gametes are digested in the stomach of the mosquito. Inside the mosquito the microgamete
will fertilized the macrogamets resulting in the formation of ookinete. The ookinetes will
transform into oocysts, the oocysts will then undergo meiosis. If these cysts will rupture it will
release sporozites that migrates to the salivary gland of the mosquito.
Epidemiology:
Geographical distribution - malaria is present worldwide in tropical and subtropical areas.
Relapse versus Recrudesence - P. vivax is, traditionally, the “relapsing” malaria. All others can
recrudesce (“bloom”) due to incomplete therapy. Prevention - detect and treat infected
individuals; mosquito control.
Immunity - incomplete immunity follows infection. Some persons get reinfected over and over.
o Sickle cell trait - the malaria parasite is not successful at utilizing the “S” haemoglobin.
This trait does not confer immunity to infection, but does offer resistance to infection.
o Duffy factor - represents the “portal of entry” antigen for P. vivax. Persons without the
factor are immune to this species (but not the others).
1. Plasmodium falciparum
Found worldwide in tropical and subtropical areas.
5. No selectivity in host erythrocytes (invades old and young RBC’s)
The infected red blood cells do not enlarge and become distorted
The trophozoite/ring is often seen in the host cells at the very edge or periphery of cell
membrane
Maurer’s dots are observed (reddish granules)
Peripheral blood smears characteristically contain only young and forms and crescent shaped
gametocytes
Can cause severe malaria because it multiples rapidly in the blood, and can thus cause severe
blood loss (anemia).
The infected parasites can clog small blood vessels. When this occurs in the brain, cerebral
malaria results, a complication that can be fatal.
2. Plasmodium vivax
Found mostly in Asia, Latin America, and in some parts of Africa.
Most prevalent human malaria parasite
Selective (young immature erythrocytes)
Infected RBC’s are usually enlarged and contains numerous schuffner’s dots (pinkish
granules)
The trophozoite is ring shaped but amoeboid in appearance
The gametocytes are round
Have dormant liver stages ("hypnozoites") that can activate and invade the blood ("relapse")
several months or years after the infecting mosquito bite.
3. Plasmodium malariae
Found worldwide, is the only human malaria parasite species that has a quartan cycle (three-
day cycle). (The three other species have a tertian, two-day cycle.)
Can infect only mature erythrocytes with relatively rigid cell membrane
The parasite’s shape must conform to the size and shape of the cell, this requirement
produces no RBC enlargement or distortion resulting in host cells’ “dark and band form” as well
as a very compact dark staining forms
In some chronically infected patients P. malariae can cause serious complications such as the
nephrotic syndrome.
4. Plasmodium Ovale
Found mostly in Africa (especially West Africa) and the islands of the western Pacific.
Selectivity for young, pliable erythrocytes
The host cell becomes enlarged and distorted, usually in an oval form
The infected cell border is commonly fimbriated or ragged
Shuffner’s dots appear as pale pinkish granules
In comparison:
6. Uncomplicated Malaria (malaria attack lasts 6-10 hours)
Stages:
A cold stage (sensation of cold, shivering)
A hot stage (fever, headaches, vomiting; seizures in young children)
And finally a sweating stage (sweats, return to normal temperature, tiredness).
Symptoms:
Fever
Chills
Sweats
Headaches
Nausea and vomiting
Body aches
General malaise
Mild jaundice
Enlargement of the liver
Increased respiratory rate
Severe Malaria (occurs when infections are complicated by serious organ failures or abnormalities in
the patient's blood or metabolism)
Cerebral malaria, with abnormal behavior, impairment of consciousness, seizures, coma, or
other neurologic abnormalities
Severe anemia due to hemolysis (destruction of the red blood cells)
Hemoglobinuria (hemoglobin in the urine) due to hemolysis
Acute respiratory distress syndrome (ARDS), an inflammatory reaction in the lungs that
inhibits oxygen exchange, which may occur even after the parasite counts have decreased in
response to treatment
Abnormalities in blood coagulation
Low blood pressure caused by cardiovascular collapse
Acute kidney failure
Hyperparasitemia, where more than 5% of the red blood cells are infected by malaria
parasites
Metabolic acidosis (excessive acidity in the blood and tissue fluids), often in association with
hypoglycemia
Hypoglycemia (low blood glucose). Hypoglycemia may also occur in pregnant women with
uncomplicated malaria, or after treatment with quinine.
Other Manifestations:
Neurologic defects may occasionally persist following cerebral malaria, especially in children.
Such defects include trouble with movements (ataxia), palsies, speech difficulties, deafness,
and blindness.
Recurrent infections with P. falciparum may result in severe anemia.
Malaria during pregnancy (especially P. falciparum) may cause severe disease in the
mother, and may lead to premature delivery or delivery of a low-birth-weight baby.
On rare occasions, P. vivax malaria can cause rupture of the spleen.
Nephrotic syndrome (a chronic, severe kidney disease) can result from chronic or repeated
infections with P. malariae.
Hyperreactive malarial splenomegaly (also called "tropical splenomegaly syndrome") occurs
infrequently and is attributed to an abnormal immune response to repeated malarial infections.
The disease is marked by a very enlarged spleen and liver, abnormal immunologic findings,
anemia, and a susceptibility to other infections (such as skin or respiratory infections).
Diagnosis:
Microscopy
PCR
IFA
Serological Test
RDT
7. Treatments:
chloroquine
atovaquone-proguanil
artemether-lumefantrine
mefloquine
quinine
quinidine
doxycycline (used in combination with
quinine)
clindamycin (used in combination with
quinine)
primaquine is active against the dormant
parasite liver forms (hypnozites) and
prevents relapses
d. Babesia sp.
Babesia bigemina /Babesia microfti
Causes babesiosis
Occurs in the US
Infects and destroys red blood cells
Transmitted by ticks (under nymph stage)
Transmission: bite of infected tick, blood transfusion, and congenital transmission
Hosts:
Definitive host –tick
Intermediate host- humans
Life Cycle:
During a blood meal, a Babesia-infected tick introduces sporozoites into the mouse host.
Sporozoites enter erythrocytes and undergo asexual reproduction (budding). In the blood, some
parasites differentiate into male and female gametes. The definitive host is the tick. Once ingested by
an appropriate tick, gametes unite and undergo a sporogonic cycle resulting in sporozoites.
Humans enter the cycle when bitten by infected ticks. During a blood meal, a Babesia-infected tick
introduces sporozoites into the human host. Sporozoites enter erythrocytes and undergo asexual
replication (budding).Humans usually are dead-end hosts. However, human-to-human transmission is
well recognized to occur via contaminated blood transfusions.
Symptoms:
presence of hemolytic anemia and
nonspecific flu-like symptoms (e.g.,
fever, chills, body aches, weakness,
fatigue).
Splenomegaly
Hepatomegaly
Jaundice
Severe cases
Thrombocytopenia
Myocardial infarction
Renal failure
Altered mental status
Death.
8. Diagnosis:
Microscopy
Serological test
Treatment:
atovaquone PLUS azithromycin
clindamycin PLUS quinine
Phylum Ciliophora
General Characteristics:
Largest free living multi-cellular organism
Have two kinds of nuclei, a large macronucleus and a smaller micronucleus
Have cilia that are similar to but shorter than flagella
The cilia are arranged in precise rows on the cell. They are moved in unison to propel the cell
through its environment and to bring food particles to the mouth
Reproduce asexually by mitosis and sexually by conjugation
The only ciliate is a human parasite in Balantidium coli, The causative agent of a rare type of
dysentery- balantidine dysentery (infection rate is 1%)
a. Balantidium coli
Found in the intestinal tract of arthropods and some vertebrates, including mammals
Pathogen of humans, pigs, and monkeys
Coarse cilia line the peristomal area
Macronucleus is typically elongated and kidney shaped, while the micronucleus is spherical
The trophozoite inhabits the cecum, and colon of humans and is the largest protozoan parasite
to humans
Infection occurs when contaminated water or food is ingested
Excystation occurs in the small intestine and encystation occurs in the large intestine, it may
also occur outside the host
Life Cycle:
Symptoms:
Asymptomatic
Persistent diarrhea
Occasionally dysentery
Abdominal pain
Weight loss