3. DISCRIPTION
It was first invented by a scientist Franz von soxhelt. It was invented for
quantification of fat in milk. This is frequently used for the extraction of lipids in
chemistry and agriculture. A soxhelt extracter is lab equipment designed for
processing certain kinds of solids.
These devices allow for
Continuous treatment of a
sample with a solvent over a
period of hours or days to
extract compound of interest.
Typically, a soxhelt extraction is
only required where the
desired compound has a
limited solubility In a solvent ,
and the impurity is Insoluble
in that solvent
4. COMPONENTS
stirrer bar
still pot(the still pot should not be overfilled and the volume of solvent in
the still pot should be 3 to 4 times the volume of the soxhelt chamber)
Distillation path
Thimble
Solid
Siphon top
Siphon exit
Expansion adapter
Condenser
Cooling water in
Cooling water out
5. PROCEDURE
Normally a solid material containing some of the desired compound is placed inside
a thimble made from thick filter paper, which is loaded into the main chamber of
the soxhelt extractor. The soxhelt extractor is placed onto a flask containing the
extraction solvent. The soxhelt is then equipped with a condenser. The solvent is
heated to reflux. The solvent vapour travels up a distilation arm, and floods into
the chamber housing the thimble of solid. The condensor ensures that any solvent
vapour cools, and drips back down into the
Chamber housing the solid material. The chamber
containignthe solid material slowly fills with warm
solvent. Some of the desired compound will then
dissolve in the warm solvent. When the soxhelt
chamber is almost full, the chamber is automatically
empitied by a siphon side arm, with the solvent
running back down to the distillation flask.
6. CONTI….
This cycle may be allowed to repeat many times, over hours a day.
During each cycle a portion of the non-volatile compounds dissolves
in the solvent.
After many cycles the desired compound is concentrated in the
distillation flask . The advantage of this system is that instead of
many portions of warm solvent being passed through a sample, just
on batch of solvent is recycled. After extraction the solvent is
removed , typically by means of a rotary Evaporator, yielding the
extracted compound. The non-soluble portion of the extracted solid
remains in the thimble, and is usually discarded
7. MEDICINAL PLANTS COMPOUNDS EXTRACTED BY HOT
CONTINUOUS EXTRACTION
1)ss Cnidoscolus aconitifolius (Chaya or tree spinach)
Family : Euphorbiaceae
Leaf Extract
Preparation
About 3 kg of the air-dried leaf was
extracted in a soxhlet extractor at 30°C
using methanol for 5 h. The extract was
concentrated under reduced pressure in
vacuum at 35°C for 30 min using a rotary
evaporator. The resulting residue called
C. aconitifolius leaf extract (CA) was
transferred to a hot air oven where it
was dried to a constant weight at 40°C.
8. CONTI….
The yield of the preparation was 10.5% (w/w). Prior to the
experiments, CA was dissolved in corn oil overnight and was
administered to the animals according to their weights.
Function
Protects against Hepatic Damage Induced by Chronic Ethanol
Administration in Wistar Rats
9. CONTI…
2) Ziziphus oenoplia (Jackal jujube)
Family:- Rhamnaceae
Leaf Extracts
Preparation
The shade dried, coarsely powdered leaf material
was extracted successively with petroleum ether,
ethyl acetate and ethanol using Soxhlet apparatus
by continuous hot percolation method. The aqueous
extract was prepared by cold maceration technique.
Then the extracts were collected, concentrated
using rotary vacuum evaporator.
11. CONTI…
3) Murraya koenigii Linn.(Murraya koenigii)
Family :- Rutaceae
extracts of dried leaves
Preparation
We have collected aqueous extract of M. koenigii through
Soxhlet apparatus by hot continuous extraction
method. The use of commercially available
Soxhlet apparatus is a convenient way to prepare
crude plant extract.
The dried and powered drug was packed. Soxhlet
apparatus is an automatic, continuous method that
does not require further manipulation. This method
is not time-consuming, as, for a standard-sized
sample (50 g), extraction time is 48 h. The yield of the
aqueous extract was 9.52%. The extract was stored in
refrigerator until further studies.
12. This method is not time-consuming, as, for a standard-
sized
sample (50 g), extraction time is 48 h. The yield of
the
aqueous extract was 9.52%. The extract was stored in
refrigerator until further studies.
Function
Act as Anti-inflammatory and analgesic
13. CONTI…
4) Acacia nilotica L.
Leaves
Preparation
The powdered plant leaves (5 g) were successively extracted with
hexane,
chloroform, and ethyl acetate. The extraction was
done hot continuous soxhlet extraction
method. The extracts were stored at -4 °C till
further uses. Chloroform extract was used for
the present study. 2.3
GC-MS analysis
Function
GC-MS analysis of chloroform extract
of Acacia nilotica L. leaves
14. 5) Leptadenia reticulata (Jiwanti, dodi)
Family :- Asclpiadaceae
Leaves
Preparation:
Leptadenia reticulata [W&A] leaves were collected from the forest of
kalakatu, Tirunelveli District, India. Taxonomic identification was made
from botanical survey of medicinal plants,
Siddha Unit, Government of India,
Palayamkottai and authenticated by
Botanist Chelladurai with the voucher specimen
No (CCRAS-168/2011).
15. Voucher specimen has been prepared and preserved in the
Department of Pharmacy, Annamalai University for the future
reference. Fresh plant leaves were shade dried at room temperature
ground into fine powder and stored in airtight containers. ground
into fine powder and stored in airtight containers. Then extracted
(amount 500 g) with solvents of increasing ethanol, for 72 hours
with each solvent, by continuous hot extraction using the soxhlet
apparatus at a preserved in a desiccator until used for further studies.
Function
Act as Phytochemical Composition and in vitro
Antimicrobial, Antioxidant
