Nexterion Spot is a twofold concentrated spotting solution that can be modified to create different solutions by adding detergents. Nexterion Spot I is identical to the base Nexterion Spot solution, while Nexterion Spot II and III are prepared by adding cetyltrimethylammoniumbromide or Triton X-100 respectively to Nexterion Spot in concentrations of 0.02%. Nexterion Spot AM I contains 6xSSC with 3 M betaine instead of Nexterion Spot, and Nexterion Spot AM II is prepared by adding betaine to Nexterion Spot. The detergent concentration can be varied between 0.01-0.1% for modifying spot size.

1. Preparation of Spotting Solutions based on
Nexterion Spot
Former Product Now
available
Preparation of twofold (2x)
solution
Remark
Nexterion Spot I
(QMT Spotting Solution I)
Nexterion
Spot
use as supplied Nexterion Spot is
identical to Nexterion
Spot I
Nexterion Spot II
(QMT Spotting Solution II)
no add 100 µl 2% Cetyl-
trimethylammoniumbromid to
10 ml Nexterion Spot
final concentration of
detergent is 0.02% in
the 2x solution
Nexterion Spot III
(QMT Spotting Solution III)
no add 20 µl 10% Triton X100 to
10 ml Nexterion Spot
final concentration of
detergent is 0.02% in
the 2x solution
Nexterion Spot AM I
(QMT Spotting Solution IV)
no 6xSSC with 3 M betaine
Nexterion Spot AM II
(QMT Spotting Solution V)
no add 10 ml 6 M betaine to
10 ml Nexterion Spot
General remarks:
Nexterion Spot is supplied as a twofold (2x) concentrated solution.
The former Nexterion Spot II and III and AM II are also 2x concentrated and are prepared
directly from the 2x Nexterion Spot.
For further modification of Nexterion Spot to adjust spot size, the concentration of detergent
may be varied between 0.01 and 0.1% in the 2x solution. Other detergents as sarcosyl,
Tween or SDS may be used too (within same concentration range).
For Technical Assistance or Ordering Inquiries, please contact:
Europe / Asia – Pacific: USA / Canada
Microarray Solutions Microarray Solutions
SCHOTT Jenaer
Glas GmbH SCHOTT North America, Inc.
Otto-Schott-Str. 13 555 Taxter Road
07745 Jena Elmsford, NY 10523
Germany USA
Phone: +49-3641-681-91969 Phone: 914-831-2251
Fax: +49-3641-681-970 Fax: 914-831-2353
E-mail: coatedsubstrate@schott.com E-mail: coatedsubstrate@us.schott.com
© October 2004