This third webinar in the series of Adverse Outcome Pathways (AOPs) was organised on Thursday 30 January 2020. It focused on the core information and telecommunication technologies applications that were built to support AOP development, management and dissemination. Tools and technical tips to facilitate scientific knowledge assembling and evaluations were also presented. The AOP framework implements a collaborative and innovative approach for collecting mechanistic knowledge from various sources that can eventually support chemical safety assessment. Clemens Wittwehr from the European Commission Joint Research Centre provided an introduction on AOP-KB.
4. The AOP community of practice in reality
Where do I find AOPs in real life?
What is an AOP in real life?
What can I do with an AOP in real
life?
Where do I get more
information?
5. https://aopkb.oecd.org/
https://www.effectopedia.org/
https://aopwiki.org/
Third Party Tools
http://apps.cytoscape.org/apps/aopxplorer
Intermediate Effects Database
(IEDB)
• Entry level module for
evaluating an AOP’s scientific
evidence
• Supports OECD review of AOPs
• Default go-to module for all
qualitative AOPs
• Main entry point for the AOP-KB
• Search engine for all AOPs
Houses the status of all AOPs & links to the official copies
• Allows browsing of review reports
• Captures quantitative
information and models
• Provides standard visual
representation of AOPs
and associated test
methods
The AOP Knowledge Base
On the Web this Summer 2020!
6. The AOP community of practice in reality
Where do I find AOPs in real life?
In the AOP Knowledge Base
Wiki!
https://aopwiki.org
What is an AOP in real life?
What can I do with an AOP in real
life?
Where do I get more
information?
8. The AOP community of practice in reality
Where do I find AOPs in real life?
In the AOP Knowledge Base
Wiki!
https://aopwiki.org
What is an AOP in real life?
A document in the Wiki
What can I do with an AOP in real
life?
Where do I get more
information?
9. AOP Page
Section 1 – Title
Section 2 – Authors
Section 3 - Status
Section 4 – Abstract
Background (Optional)
Section 5a – Summary of the AOP
MIE
KEs
AO
Key Event Relationships
Applicability domain(s) of the AOP
Life-stage
Taxonomic
Sex
Section 7 – Overall Assessment of the AOP
Modified Bradford Hill Considerations
KE Pages
• Description
• Measurement/ detection
• Taxonomic applicability
AO Page
• Description
• Measurement/ detection
• Taxonomic applicability
• Regulatory relevance
KER Pages
• Title
• Description
• Biological plausibility
• Empirical support
• Inconsistencies and
uncertainties
• Quantitative
understanding
Linkage table
MIE Page
• Description
• Measurement/ detection
• Taxonomic applicability
• Evidence for chemical
initiation
Chemical initiator(s)
Section 8 – Considerations for Potential Applications of the AOP
AOP Wiki documents
10. The AOP community of practice in reality
Where do I find AOPs in real life?
In the AOP Knowledge Base
Wiki!
https://aopwiki.org
What is an AOP in real life?
A document in the Wiki
What can I do with an AOP in real
life?
View online and/or Print
Comment
Edit
Review
Where do I get more
information?
11. • Open to anyone, no account requiredRead access
• Create account, no approvals requiredCommenting
• Create account (same as above)
• Submit brief developer
application for approval
Author/write access
• Help ensure consistency with published principles and OECD guidelines
• Monitor the use of vocabulary (ontologies) to facilitate re-use and the creation
of networks
Gardener (experienced
AOP developers/ wiki
users)
Accessing the AOP Wiki
https://aopwiki.org/
http://www.saaop.org/
• AOP peer review
• Volunteers needed!!!Reviewer
12. The AOP community of practice in reality
Where do I find AOPs in real life?
In the AOP Knowledge Base
Wiki!
https://aopwiki.org
What is an AOP in real life?
A document in the Wiki
What can I do with an AOP in real
life?
View online and/or Print
Comment
Edit
Review
Where do I get more
information?
On the OECD website!
Just google "OECD AOPs"
13. AOP
AOP
AOP
AOP
AOP
AOPAOP
AOP
AOP
AOP
AOP AOP
AOP
AOP
AOP AOP
AOP
AOP AOPAOP
AOP
SAAOP Entrance Gate
AOP-KB write access via SAAOP
AOPs from the scientific community
OECD Entrance Gate
AOP-KB write access via SAAOP
AOPs get directly on the OECD work program
AOP
EAGMST check
Closer look at selected AOPs
Support given to authors
First reality check
External Review
External review organised by TFHA/WNT
The AOP lifecycle
15. AOP-KB in context
Test Methods
Adverse Outcome
Pathways
Undergoes test
OHT 201
Chemical
triggers
doesnottrigger
triggers
doesnottrigger
inconclusiveFor example
Ives C, Campia I, Wang RL, Wittwehr C, Edwards S. Creating a Structured AOP
Knowledgebase via Ontology-Based Annotations. Appl In Vitro Toxicol. 2017 Dec
1;3(4):298-311. doi: 10.1089/aivt.2017.0017
Currently
being
implemented