Pharmacology (effects of drugs on the ciliary motility of forg oesophagus)

1. Pharmacology practical
(Effects of drugs on the ciliary motility of forg oesophagus)
By : Osama Al-Zahrani
@OSAMA_Z96

2. Introduction :
- Cholinergic drugs causes contraction of cilia leading to increased movements.
- Anticholinergics drugs causes paralysis of cilia leading to decreased movements.
Objective :
- To find out the action of certain drugs on the ciliary motility of frog oesophagus.
Materials :
1- Frog.
2- Poppy seeds.
3- Frog wooden board.
4- Stop watch
5- Drugs & Solution :
A) Acetylcholine 10% : Cholinergic agonist.
B) Physostigmine 10% : Anti-choline esterase inhibitor.
C) Atropine 0.1% : Non-selective muscarinic agonist, or Cholinergic antagonist.
D) Frog Ringer : Nutrient solution To keep the tissue viable.

3. Materials pictures
Frog wooden board Poppy seeds
In Situ experiment

4. Experiment results
MeanReading 3Reading 2Reading 1DrugSerial
32 s33 s29 s34 sRinger (Control)1
19 s17 s18 s22 sAcetylcholine2
14 s14 s14 s14 sPhysostigmine3
46 s51 s46 s43 sAtropine4

5. 0
5
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Experiment results
Ringer (Control) Acetylcholine Physostigmine Atropine

6. Interpretation of the results
- The most effective drug is Physostigmine,
because the drug take short time to increase the movement.
- The order of the drugs depending on the shortest time as following :
1- Physostigmine
2- Acetylcholine
3- Atropine