A presentation on the Stem Cells 21 - IntelliHealthPlus medical center in Bangkok, Thailand. Information on Umbilical cord mesenchymal stem cells and Cd34+ cells, also the companies Ultrasound adipose stem cell separation.

1. STEMCELLS21: WHO WE ARE

2. StemCells21 is a Biotech company based in Bangkok, Thailand. The
company was established in 2008, founded by Mr. Paul Collier, with
the first Regenerative Medicine facility & lab opening its doors in
2011. StemCells21 offers:
• Premium Regenerative Medicine @ IntellliHealth+
• Allogenic Stem Cells Supply & Technology
• Autologous Stem Cells in-house laboratory services
• Product/Protocol R&D: Serum & Essentials
STEMCELLS21: THE COMPANY

3. IntelliHealth+ centres were established to offer patients stem cell
based treatment programs, in 5* modern facilities. When patients
undergo a treatment program at IH+, an appropriate stem cell
source is selected and a supportive therapy program is designed.
Stem Cell Sources: Umbilical Cord, Peripheral Blood or Adipose.
Supportive Therapies: Peptides/Growth factors, Bio-photon laser
treatments, Oxygen therapies and Nutrition/Enzyme/Hormones.
STEMCELLS21: THE FACILITIES

4. STEMCELLS21: THE FACILITIES

5. STEMCELLS21: THE FACILITIES

6. STEMCELLS21: STEM CELLS
What Are Stem Cells?
Stem cells are biological cells that can an self renew to produce
more stem cells and differentiate into specialised cell types.

7. Self Renewal:
Differentiation:
STEMCELLS21: STEM CELLS

8. STEMCELLS21: STEM CELLS
How stem cells work
These cells are capable of performing three important functions with
unique abilities:
- Plasticity: Potential to change into other cell types like nerve cells
- Homing: To travel to the site of tissue damage or under stimulation
- Engraftment: To unite with other tissues

9. Umbilical Cord-Derived Stem Cells
STEMCELLS21: STEM CELLS

10. Umbilical Cord-Derived Stem Cells
STEMCELLS21: STEM CELLS

11. Corporate Policies: ISO 14001:2004
BSC Safety Audit: British Safety Council
Clinical Research Services: National GLP, GLP
CAP (College of American Pathologies)
Regenerative Medicine: AABB (Accredited Cord
Blood Facility)
Molecular Medicine: CAP, NABL, CLIA
STEMCELLS21: CELL LAB

12. Mesenchymal & CD34+ Cells:
Fully characterised, early passage stem cells are
available ready for use in cryo-storage.
All products undergo extensive testing including
characterisation studies. This gives detailed
information on genetic stability, gene expression, and
differentiation potential.
STEMCELLS21: MSC & CD34+

13. Source: human umbilical cord-derived
▪Mesenchymal stem cells are multipotent stem cells
that can differentiate into a number of cell types
including osteoblasts, chondrocytes, myocytes,
adipocytes, neurons, etc.
▪These can have immense applications in
regenerative medicine.
STEMCELLS21: MSC

14. ▪Early passage: maximizing stable life-span
▪Spindle morphology consistent with bone marrow
MSCs
▪Fully characterised with a panel of cell surface
markers
STEMCELLS21: MSC

15. ▪Passage #: P2 or P3
▪Source: Donated human umbilical cords
▪Microbiological tests: Maternal blood and cord blood tested
negative for HIV I&II, HCV, CMV IgG/IgM, syphilis IgG/IgM
antibodies and Hepatitis B surface antigen. Free of bacterial
and fungal contamination.
▪Morphology: Adherent cells having spindle morphology
▪Representice surface markers: CD31-, vWF- , CD29+,
CD44+, CD14-, SSEA4+, HLADR_, HLA ABC+, CD73+,
CD105+, CD24-
▪Endotoxin Content by LAL Test <10 EU/ml
▪pH of the Medium 7.4 ±0.2
▪Average Pre-Freeze Viability >90%
▪Average Purity >90%
STEMCELLS21: MSC

16. ▪Source: human umbilical cord blood-derived
▪CD34 cells are lymphohematopoietic stem and
progenitor cells, scaleable up to 10-folds in culture,
and capable of maintaining long-term
hematopoiesis.
▪They can be used for a variety of purposes
including reconstitution of myeloablated marrow,
neurological and angiogeneic applications.
STEMCELLS21: CD34+

17. ▪Early passage: maximizing stable life-span
▪Good morphology: non-adherent,translucent and
round cells
▪Fully characterised with a panel of cell surface
markers
STEMCELLS21: CD34+

18. ▪Passage #: P1 or P2
▪Source: Human umbilical cord blood of donated umbilical
cords.
▪Microbiological tests: Maternal blood and cord blood tested
negative for HIV I&II, HCV, CMV IgG/IgM, syphilis IgG/IgM
antibodies and Hepatitis B surface antigen. Free of bacterial
and fungal contamination.
▪Morphology: Non adherent translucent round cells
▪Representive surface markers: CD33+, CD38+, CD34+
▪Endotoxin Content by LAL Test <10 EU/ml
▪pH of the Medium 7.4 ±0.2
▪Average Pre-Freeze Viability >90%
▪Average Purity >90%
STEMCELLS21: CD34+

19. Autologous Adipose Tissue-derived
Stromal Vascular Fraction
STEMCELLS21: ADIPOSE SVF

20. STEMCELLS21: ADIPOSE SVF

21. How it Works:
Our technology process yields SVF which is a functionally
diverse population of cells that is believed to be synergistic
and able to communicate with other cells in their local
environment.
Ultrasonic Cavitation Separation process is:
• Clean/Safe – no animal products or
enzymes
• Efficient– complete time 30 - 40
minutes with cell counting
• High number of SVF
• Extra cellular matrix present which
aids in the regenerative process
• Can be applied to a variety of
diseases
STEMCELLS21: ADIPOSE SVF

22. STEMCELLS21: ADIPOSE SVF
Prior to our process, the cell membrane's are clearly defined
Contents of the Adipose cell begin to emulsify.
Emulsifications of the fats and water content to occur.
Homogenization of the Adipose tissue has occurred!

23. ▪Derived from Blood Vessels in adipose (fat) tissue
▪Capillaries are primary source (see inset below)
▪Same-day clinical procedure
▪Easy to harvest fat
▪High cell counts
Blood vessels
Adipose (fat) cells
Capillaries
The isolated SVF contains:
▪ Adult Stem Cells
▪ Endothelial Cells
▪ Fibroblast Cells
▪ Pericytes
▪ Red Blood Cells
▪ White Blood Cells
… and much more
STEMCELLS21: ADIPOSE SVF

24. Varma et al, Stem Cells and Development 2007:91-104 (freshly isolated adipose). Repeated by
Collas et al and Yoshimura et al.
CD90+ positive cells after ultrasonic cavitation
STEMCELLS21: ADIPOSE SVF

25. Fraser JK, Wulur I. Fat tissue: an underappreciated
source of stem cells for biotechnology. Trends in
Biotechnology. 2006;24:150-154.
Dapi staining of lipoaspirate. Dapi nuclear stain
highlights the capillaries containing MSC’s
STEMCELLS21: ADIPOSE SVF

26. Extracellular matrix (ECM)
• Basement membrane (type 4 reticular collagen)
• Interstitial matrix (Type 1 collagen)
ECM regulates cell function and is a reservoir of growth
factors. Triggering protease activity releases growth factors
ECM controls growth, wound healing and fibrosis; is able to
block the immune reaction of inflammation and scarring to
allow replacement tissue to grow in the absence of scarring.
STEMCELLS21: ADIPOSE SVF

27. STEMCELLS21: ADIPOSE SVF

28. 0.4-1 Million Cells per gram of adipose
15–30 % Dead Cells
Digests ECM
More adipose required (more time)
Animal or bacterial product
8-16 Million Cells per gram of adipose
5-15 % Dead Cells
Preserves ECM
Less adipose required (less time)
Clean and safe
Ultrasonic Cavitation Proprietary Technology
! Collagenase Enzyme
STEMCELLS21: ADIPOSE SVF

29. Autologous EPCs & APCs:
Endothelial Progenitor Cells & Angiogenic Precursor Cells:
Prepared from patients whole blood cultured for specific cell type EPCs & APCs for
regeneration of vascular.
Key areas of treatment: Vascular disease

30. Immunotherapy Cell Therapies:
Autologous natural killer cells (NK), Cytokine induced killer cells (CIK) & Dendritic
(DC) vaccines derived from the Patients blood. Delivered in multiple infusion
programs.
Key areas of treatment: Cancers, Viral Infections and Immune system disorders.

31. Fibroblasts:
1) Autologous cultured fibroblasts for wound healing and cosmetic skin rejuvenation
2) Neo-natal ready available fibroblasts for wound healing and cosmetic skin
rejuvenation
Plasma-Derived:
PRP preparations for a variety of conditions: Skin Rejuvenation, Orthopaedic,
Muskuloskelatal Pain