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High Pressure Processing of Food
(Non-thermal treatment)
Presented by:
Praween Nishad
Ph.D. I Year
Department of Process Food Engineering
INDIAN AGRICULTURAL RESEARCH INSTITUTE, NEW DELHI
High Pressure Processing (HPP)
HPP is also termed as
Hyperbaric pressure
Ultra high pressure
High hydrostatic pressure
Pascalization
Inactivate
harmful
pathogen,
microorganis
m
Commonly
400-600
MPa
used
Food is subjected to elevated pressures
(upto 900 MPa) with or without the
addition of heat.
Kadam et al., 2012)
Biological,
no additives
Safe treatment
in final
packaging
Minimum
changes in
taste, color
and flavor
Clean,
no waste,
no trash
More shelf life,
better distribution
Advantages
More efficient
method
Revolution
1994
(Jams & Jellies)
1899
Japanese
Scientist Hite
(milk 680
MPa for 10
min)
1914
Fruits & Vegetables
1996
Europe & US
2001
Australia
Commercialized in world wide
HISTORY
Source: Thakur & Nelson, 1998; , Lerasle et al. 2012
42%
7 %
1 %
32%
14%
4 %
32 %
27 %
16%
14%
11
%
Fruits & Vegetables
Meat
Seafood
Juice & Beverages
Other
Global High Pressure Processing Market
The global High Pressure Processing market is projected to reach USD 1.1 billion by the end
of 2023.
Source: https://blog.hiperbaric.com/en/global-trends-in-hpp-for-2019
Process Principles
Iso-static or Pascal’s law Principle of Le Chatelier & microscopic reordering
Source: Frakas & Hoover, 2000
First High Pressure Food Processing Equipment
is the first company to design HPP
equipment & its work at pressures
up to 700 MPa.
Source: Hite, B 1899; Palou E 1997
Direct compression
Indirect compression
How To Generate High Pressure
Source: Mertens 1995
How To Process
Source: Caner et al., 2004; Bermúdez & Cánovas, 2011
100 200 300 400 500 600 700 800 900
5
10
15
Pressure (MPa)
Dwell
time
(min.)
Pasteurization
Sterilization
Process Parameters
Source: Sizer et al., 2002; Borda et al 2013
Milk & milk
products
Meat products
Fruits &
vegetable juices
100 200 300 400 500 600 700 800 900
5
20
30
40
10
Pressure (MPa)
Holding
time
(min)
HPP Products & Process Parameters
Source; Verma, DK 2020
300-400 MPa
> 1000 MPa
Molds
Yeasts
Parasites
Bacterial
spores
Source: Knorr 1995; Georget et. al., 2015
At 50 MPa – Inhibit protein synthesis
< 250 MPa – damage to cell membrane
internal cell structure
>250 MPa – induce irreversible protein denaturation,
rupture to cell membrane &
excretion on internal membrane
How to inactivate micro-organism
Flexible packaging (>15% volume contraction)
Tear strength, puncture resistance and surface smoothness
Required air tight, rounded & reinforced edges.
Minimal head space.
Often flexible pouches or bottles are inserted in secondary cardboard.
What kind of packaging works well with HPP
Disadvantages
High capital cost of equipment
Require very high pressure for
inactivation of enzymes & bacterial
spores
Most of the pressure processed
foods need low temperature
storage
Issues & challenges in HPP of food
The main difficulty in monitoring due to lack of data on thermo-physical
properties under pressure.
More research is needed to evaluate pressure uniformity within pressure vessels
of larger volume.
The assumption that all foods follow the iso-static rule is also not well accepted.
Change in P transmitting fluid T as a result of compression heating and
subsequent heat transfer should be considered in process modeling.
The change in density at the geometric centre of food may experience different
pressure.
Case Study - 1
Materials and Methods
Harvested & Stored at 2°C Washed & Manually peeled Packed in LDPE (80μm)
Independent variables
100 MPa 10
min
15
min
5
min
200 MPa 10
min
15
min
5
min
300 MPa 10
min
15
min
5
min
Texture Analyzer
Refractometer
Colorimeter
Voss, (1992), Mahajan & Goswami (2004), Ranganna, (2007)
Equipments used for analysis
Results and Discussions
Effect on the color parameters of litchi fruit
0
1
2
3
4
5
6
5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min
100 MPa 200 MPa 300 MPa
Color difference
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
5 min 10 min15 min 5 min 10 min15 min 5 min 10 min15 min
100 MPa 200 MPa 300 MPa
Browning index
44
46
48
50
52
54
56
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Lightness
0
1
2
3
4
5
6
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Chroma
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Hue
Figure . Changes in ΔE and BI of HP-treated litchi during refrigerated storage.
Figure . Changes in DL and AA of HP-treated litchi during storage.
Figure . Changes in pH and TSS of HP-treated litchi during refrigerated storage
0.0
0.5
1.0
1.5
2.0
2.5
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Gumminess (N)
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Chewiness (N)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Firmness (N)
0.28
0.29
0.30
0.31
0.32
0.33
0.34
0.35
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Cohesiveness
0.88
0.89
0.90
0.91
0.92
0.93
0.94
0.95
0.96
0.97
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Springiness
Effect on the texture profile of litchi fruit
0.00
1.00
2.00
3.00
4.00
5.00
6.00
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Aerobic mesophiles
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Psychrotrophs
5
min
10
min
15
min
5
min
10
min
15
min
5
min
10
min
15
min
UT 100 MPa 200 MPa 300 MPa
Yeast & Mold
Effect on the microbial counts of litchi fruit
Figure . Changes in microbial count HP-treated litchi during storage.
Parameters Day of storage Estimated shelf life
Untreated 17 12
Pressure (MPa) Dwell time (min)
100
5 12 9
10 17 12
15 22 17
200
5 22 17
10 22 17
15 27 22
300
5 22 17
10 42 32
15 42 32
Table: Effect on HPP treatment on shelf life of litchi fruit
Case Study - 2
Materials and Methods
20%
20%
20%
15%
5%
19.9%
Process parameters
350 MPa 5
min
450 MPa 5
min
600 MPa 3
min
85°C 7
min
HPP Combination Thermal treatment
Best
Comparing
PPO: - Polyphenol oxidase; POD: - Peroxidase; PME: -
Pectinmethylesterase.
Table: Effect of the treatments (HPP vs. MH) on the enzymatic activity
of smoothies kept at 4°C for one day after treatment
Result and Discussions
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Aerobic mesophillic
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Psychrophillic
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Yeast & Molds
Effect on the microbial counts
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Polyphenol oxidase (%)
0.0
20.0
40.0
60.0
80.0
100.0
120.0
140.0
160.0
180.0
200.0
1D 7D 14D21D28D 1D 7D 14D21D28D
HPP MH
Peroxidase (%)
0.0
0.1
0.1
0.2
0.2
0.3
0.3
0.4
0.4
0.5
0.5
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Pectin methyl esterase (μ/g)
Effect on the enzymatic activity
33.2
33.4
33.6
33.8
34.0
34.2
34.4
34.6
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Ligthness
0
5
10
15
20
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Chroma
60.0
62.0
64.0
66.0
68.0
70.0
72.0
74.0
76.0
78.0
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Hue
0.00
0.02
0.04
0.06
0.08
0.10
0.12
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Browning index
Effect on the color parameters
Effect on the physical properties
0
5
10
15
20
25
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Soluble solids (brix)
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Insoluble solids (g/100g)
0.0
0.5
1.0
1.5
2.0
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Abs. viscosity (cP)
0
10
20
30
40
50
60
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Transmittance (%)
0
500
1000
1500
2000
2500
3000
3500
1D 7D 14D 21D 28D 1D 7D 14D 21D 28D
HPP MH
Turbidity (NTU)
HPP was found to be effective in stabilizing the color, structural, biochemical and
microbiological changes.
Shelf life was extended 2 – 3 times as compared to untreated.
Pressurized smoothies show earlier clarification and oxidation, so, it will be
necessary to carefully select raw materials.
Improve pressurization conditions to achieve a better stabilization during further
chilled storage.
Chemical and structural changes related to microbial activity were minimized during
storage.
Conclusions
1. B. H. Hite, "The effect of pressure in the reservation of milk,” Bull. West Virginia Univ. Agric. Exper. Stn., vol. 58, pp. 15-35, 1899.
2. E. Palou, Non-thermal preservation of foods vol. 82. New York: CRC Press, 1997.
3. Adriana Hurtado, Maria Dolors Guàrdia, Pierre Picouet, Anna Jofré, Sancho Bañón, José María Ros, Shelf‐life extension of
multi‐vegetables smoothies by high‐pressure processing compared with thermal treatment. Part I: Microbial and enzyme inhibition,
antioxidant status, and physical stability. J Food Process Preserv. 2019;00:e14139.
4. Neelima Kaushik, Barjinder P Kaur and P Srinivasa Rao. Application of high pressure processing for shelf life extension of litchi
fruits (Litchi chinensis cv. Bombai) during refrigerated storage. Food Science and Technology International, 1–15, 2013.
5. https://blog.hiperbaric.com/en/global-trends-in-hpp-for-2019
6. DK Verma, M Thakur, J. Kumar, PP Srivastav, ARS Al-hilphy, AR. Patel & HAR Suleria. High Pressure Processing (HPP):
Fundamental Concepts, Emerging Scope, and Food Application. 2020.
7. Hsiao-Wen Huang , Sz-Jie Wu , Jen-Kai Lu , Yuan-Tay Shyu , Chung-Yi Wang . Current status and future trends of high-pressure
processing in food industry. Food Control 72 (2017) 1-8
8. Zuzana Smeets Kristkova , Delia Grace (ILRI) Marijke Kuiper. (Wageningen Economic Research) The economics of food safety in
India – a rapid assessment. 2017
References
Thank you

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High pressure processing of foods

  • 1. High Pressure Processing of Food (Non-thermal treatment) Presented by: Praween Nishad Ph.D. I Year Department of Process Food Engineering INDIAN AGRICULTURAL RESEARCH INSTITUTE, NEW DELHI
  • 2. High Pressure Processing (HPP) HPP is also termed as Hyperbaric pressure Ultra high pressure High hydrostatic pressure Pascalization Inactivate harmful pathogen, microorganis m Commonly 400-600 MPa used Food is subjected to elevated pressures (upto 900 MPa) with or without the addition of heat. Kadam et al., 2012)
  • 3. Biological, no additives Safe treatment in final packaging Minimum changes in taste, color and flavor Clean, no waste, no trash More shelf life, better distribution Advantages More efficient method
  • 4. Revolution 1994 (Jams & Jellies) 1899 Japanese Scientist Hite (milk 680 MPa for 10 min) 1914 Fruits & Vegetables 1996 Europe & US 2001 Australia Commercialized in world wide HISTORY Source: Thakur & Nelson, 1998; , Lerasle et al. 2012
  • 5. 42% 7 % 1 % 32% 14% 4 % 32 % 27 % 16% 14% 11 % Fruits & Vegetables Meat Seafood Juice & Beverages Other Global High Pressure Processing Market The global High Pressure Processing market is projected to reach USD 1.1 billion by the end of 2023. Source: https://blog.hiperbaric.com/en/global-trends-in-hpp-for-2019
  • 6. Process Principles Iso-static or Pascal’s law Principle of Le Chatelier & microscopic reordering Source: Frakas & Hoover, 2000
  • 7. First High Pressure Food Processing Equipment is the first company to design HPP equipment & its work at pressures up to 700 MPa. Source: Hite, B 1899; Palou E 1997
  • 8. Direct compression Indirect compression How To Generate High Pressure Source: Mertens 1995
  • 9. How To Process Source: Caner et al., 2004; Bermúdez & Cánovas, 2011
  • 10. 100 200 300 400 500 600 700 800 900 5 10 15 Pressure (MPa) Dwell time (min.) Pasteurization Sterilization Process Parameters Source: Sizer et al., 2002; Borda et al 2013
  • 11. Milk & milk products Meat products Fruits & vegetable juices 100 200 300 400 500 600 700 800 900 5 20 30 40 10 Pressure (MPa) Holding time (min) HPP Products & Process Parameters Source; Verma, DK 2020
  • 12. 300-400 MPa > 1000 MPa Molds Yeasts Parasites Bacterial spores Source: Knorr 1995; Georget et. al., 2015 At 50 MPa – Inhibit protein synthesis < 250 MPa – damage to cell membrane internal cell structure >250 MPa – induce irreversible protein denaturation, rupture to cell membrane & excretion on internal membrane How to inactivate micro-organism
  • 13. Flexible packaging (>15% volume contraction) Tear strength, puncture resistance and surface smoothness Required air tight, rounded & reinforced edges. Minimal head space. Often flexible pouches or bottles are inserted in secondary cardboard. What kind of packaging works well with HPP
  • 14. Disadvantages High capital cost of equipment Require very high pressure for inactivation of enzymes & bacterial spores Most of the pressure processed foods need low temperature storage
  • 15. Issues & challenges in HPP of food The main difficulty in monitoring due to lack of data on thermo-physical properties under pressure. More research is needed to evaluate pressure uniformity within pressure vessels of larger volume. The assumption that all foods follow the iso-static rule is also not well accepted. Change in P transmitting fluid T as a result of compression heating and subsequent heat transfer should be considered in process modeling. The change in density at the geometric centre of food may experience different pressure.
  • 17. Materials and Methods Harvested & Stored at 2°C Washed & Manually peeled Packed in LDPE (80μm)
  • 18. Independent variables 100 MPa 10 min 15 min 5 min 200 MPa 10 min 15 min 5 min 300 MPa 10 min 15 min 5 min
  • 19. Texture Analyzer Refractometer Colorimeter Voss, (1992), Mahajan & Goswami (2004), Ranganna, (2007) Equipments used for analysis
  • 21. Effect on the color parameters of litchi fruit 0 1 2 3 4 5 6 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min 100 MPa 200 MPa 300 MPa Color difference 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 5 min 10 min15 min 5 min 10 min15 min 5 min 10 min15 min 100 MPa 200 MPa 300 MPa Browning index 44 46 48 50 52 54 56 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Lightness 0 1 2 3 4 5 6 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Chroma 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Hue
  • 22. Figure . Changes in ΔE and BI of HP-treated litchi during refrigerated storage.
  • 23. Figure . Changes in DL and AA of HP-treated litchi during storage.
  • 24. Figure . Changes in pH and TSS of HP-treated litchi during refrigerated storage
  • 25. 0.0 0.5 1.0 1.5 2.0 2.5 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Gumminess (N) 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Chewiness (N) 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Firmness (N) 0.28 0.29 0.30 0.31 0.32 0.33 0.34 0.35 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Cohesiveness 0.88 0.89 0.90 0.91 0.92 0.93 0.94 0.95 0.96 0.97 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Springiness Effect on the texture profile of litchi fruit
  • 26. 0.00 1.00 2.00 3.00 4.00 5.00 6.00 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Aerobic mesophiles 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Psychrotrophs 5 min 10 min 15 min 5 min 10 min 15 min 5 min 10 min 15 min UT 100 MPa 200 MPa 300 MPa Yeast & Mold Effect on the microbial counts of litchi fruit
  • 27. Figure . Changes in microbial count HP-treated litchi during storage.
  • 28. Parameters Day of storage Estimated shelf life Untreated 17 12 Pressure (MPa) Dwell time (min) 100 5 12 9 10 17 12 15 22 17 200 5 22 17 10 22 17 15 27 22 300 5 22 17 10 42 32 15 42 32 Table: Effect on HPP treatment on shelf life of litchi fruit
  • 31. Process parameters 350 MPa 5 min 450 MPa 5 min 600 MPa 3 min 85°C 7 min HPP Combination Thermal treatment Best Comparing
  • 32. PPO: - Polyphenol oxidase; POD: - Peroxidase; PME: - Pectinmethylesterase. Table: Effect of the treatments (HPP vs. MH) on the enzymatic activity of smoothies kept at 4°C for one day after treatment Result and Discussions
  • 33. 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Aerobic mesophillic 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Psychrophillic 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Yeast & Molds Effect on the microbial counts
  • 34. 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Polyphenol oxidase (%) 0.0 20.0 40.0 60.0 80.0 100.0 120.0 140.0 160.0 180.0 200.0 1D 7D 14D21D28D 1D 7D 14D21D28D HPP MH Peroxidase (%) 0.0 0.1 0.1 0.2 0.2 0.3 0.3 0.4 0.4 0.5 0.5 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Pectin methyl esterase (μ/g) Effect on the enzymatic activity
  • 35. 33.2 33.4 33.6 33.8 34.0 34.2 34.4 34.6 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Ligthness 0 5 10 15 20 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Chroma 60.0 62.0 64.0 66.0 68.0 70.0 72.0 74.0 76.0 78.0 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Hue 0.00 0.02 0.04 0.06 0.08 0.10 0.12 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Browning index Effect on the color parameters
  • 36. Effect on the physical properties 0 5 10 15 20 25 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Soluble solids (brix) 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Insoluble solids (g/100g) 0.0 0.5 1.0 1.5 2.0 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Abs. viscosity (cP) 0 10 20 30 40 50 60 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Transmittance (%) 0 500 1000 1500 2000 2500 3000 3500 1D 7D 14D 21D 28D 1D 7D 14D 21D 28D HPP MH Turbidity (NTU)
  • 37. HPP was found to be effective in stabilizing the color, structural, biochemical and microbiological changes. Shelf life was extended 2 – 3 times as compared to untreated. Pressurized smoothies show earlier clarification and oxidation, so, it will be necessary to carefully select raw materials. Improve pressurization conditions to achieve a better stabilization during further chilled storage. Chemical and structural changes related to microbial activity were minimized during storage. Conclusions
  • 38. 1. B. H. Hite, "The effect of pressure in the reservation of milk,” Bull. West Virginia Univ. Agric. Exper. Stn., vol. 58, pp. 15-35, 1899. 2. E. Palou, Non-thermal preservation of foods vol. 82. New York: CRC Press, 1997. 3. Adriana Hurtado, Maria Dolors Guàrdia, Pierre Picouet, Anna Jofré, Sancho Bañón, José María Ros, Shelf‐life extension of multi‐vegetables smoothies by high‐pressure processing compared with thermal treatment. Part I: Microbial and enzyme inhibition, antioxidant status, and physical stability. J Food Process Preserv. 2019;00:e14139. 4. Neelima Kaushik, Barjinder P Kaur and P Srinivasa Rao. Application of high pressure processing for shelf life extension of litchi fruits (Litchi chinensis cv. Bombai) during refrigerated storage. Food Science and Technology International, 1–15, 2013. 5. https://blog.hiperbaric.com/en/global-trends-in-hpp-for-2019 6. DK Verma, M Thakur, J. Kumar, PP Srivastav, ARS Al-hilphy, AR. Patel & HAR Suleria. High Pressure Processing (HPP): Fundamental Concepts, Emerging Scope, and Food Application. 2020. 7. Hsiao-Wen Huang , Sz-Jie Wu , Jen-Kai Lu , Yuan-Tay Shyu , Chung-Yi Wang . Current status and future trends of high-pressure processing in food industry. Food Control 72 (2017) 1-8 8. Zuzana Smeets Kristkova , Delia Grace (ILRI) Marijke Kuiper. (Wageningen Economic Research) The economics of food safety in India – a rapid assessment. 2017 References