1. Rameshwar Tiwari
PhD
Gender: Male
DOB: 10-Feb-1985
Marital Status: Unmarried
Email ID: tiwari.rameshwar@gmail.com
Contact Number: +91 8010150114
To attain a challenging position in an organization by utilizing my technical skills & abilities for its growth and
gaining experience at each step.
RESEARCH EXPERIENCE
 Worked as Senior Research Fellow under the project entitled ‘Increasing the efficiency of microbial
production of bioethanol from agricultural biomasses funded by National Fund for Basic, Strategic and
Frontier Application Research in Agriculture (NFBSFARA) in Division of Microbiology, Indian Agricultural
research Institute, New Delhi, India from Feb 2011 to Dec 2015.
 Worked as a Project Assistant under the project entitled ‘Design and development of database and
analytical tools for microarray data on Leishmania donovani parasites’ funded by Department of
Biotechnology (DBT) in Drug Target Discovery and Development Division, Central Drug Research Institute
(CDRI-CSIR), Lucknow, Uttar Pradesh from Feb 2010 to February 2011.
 Worked as Senior Research Fellow under the project entitled ‘Application of microorganism in agriculture
and allied sector’ funded by Indian council of agriculture research (ICAR) in National Bureau of
Agriculturally Important Microorganisms (NBAIM-ICAR), Mau, Uttar Pradesh from Sept 2007 to February
2010
 Six months Master degree research project entitled “‘Molecular and structural analysis of different
putative drug target genes from Leishmania donovani’ from Central Drug Research Institute, Lucknow
(India).
ACADEMIC QUALIFICATION
 Ph.D. (Microbiology) on ‘Bioprospecting of β-glucosidase from diverse environmental niches by
culturable and unculturable approaches’ from Maharshi Dayanand University, Rohtak and Indian
Agricultural Research Institute, New Delhi
 M.Sc. (Microbiology) from Barkatullah University, Bhopal (M.P.) 2007.
 B.Sc. (Industrial Microbiology) from Barkatullah University, Bhopal (M.P.) 2005.
 Senior Secondary from Board of High School & Intermediate Education, M.P. in 2002.
 Higher Secondary from Board of High School & Intermediate Education, M.P. in 2000.
Awards
• Young Scientist Award in Molecular Microbiology and Biotechnology by the Association of Microbiologists
of India for the year 2015.
• Received best poster award for the joint poster entitled “Comparative profiling of solid state and
submerged production of glycoside hydrolases by Aspergillus niger for enzymatic degradation of
lignocellulosic biomass” by Saritha M, Tiwari R, Singh S, Adak A, Arora A, Nain L at National seminar on
1 | P a g e

2. Recent advances in agriculture, biomedical and environmental biotechnology, held at Department of
Biotechnology, Anand Engineering Collage, Agra from May 1-2, 2015.
PAPER PUBLICATIONS
Total international publication : 21
Total impact factor (Thomson reuters) : 38.05
Total citation (Google scholar) : 137
1. Tiwari R, Nain PKS, Singh S, Adak A, Saritha M, Rana S, Sharma A, Nain L. Cold active holocellulase
cocktail from Aspergillus niger SH3: process optimization for production and biomass hydrolysis. J Taiwan
Inst Chem Eng. 2015;56:57-66. (Impact Factor: 3.0)
2. Tiwari R, Shukla P, Singh S, Nain L. Novel cold active β-glucosidase from Pseudomonas lutea BG8 suitable
for simultaneous saccharification and fermentation. RSC Adv. 2014;4:58108-58115. (Impact Factor:
3.71)
3. Tiwari R, Singh S, Nain PK, Rana S, Sharma A, Pranaw K, Nain L. Harnessing the hydrolytic potential of
phytopathogenic fungus Phoma exigua ITCC 2049 for saccharification of lignocellulosic biomass. Bioresour
Technol. 2013;150:228-34. (Impact factor: 4.49)
4. Tiwari R, Rana S, Singh S, Arora A, Kaushik R, Agrawal VV, Saxena AK, Nain L. Biological delignification of
paddy straw and Parthenium sp. using a novel micromycete Myrothecium roridum LG7 for enhanced
saccharification. Bioresour Technol. 2013;135:7-11. (Impact factor: 4.49)
5. Tiwari R, Singh S, Singh N, Adak A, Rana S, Sharma A, Arora A, Nain L. Unwrapping the hydrolytic system
of the phytopathogenic fungus Phoma exigua by secretome analysis. Process Biochem. 2014;49:1630-6.
(Impact factor: 2.52)
6. Singh A, Singh DP, Tiwari R, Kumar K, Singh RV, Singh S, Prasanna R, Saxena AK, Nain L. Taxonomic and
functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavates. Microbiol Res.
2015;175:48-56. (Impact factor: 2.56)
7. Tiwari R, Pranaw K, Singh S, Nain PKS, Shukla P, Nain L. Two step statistical optimization for cold active β-
glucosidase production from Pseudomonas lutea BG8 and its application for improving saccharification of
paddy straw. Biotechnol Appl Biochem. 2015; doi: 10.1002/bab.1415. (Impact Factor: 1.36)
8. Singh S, Tiwari R, Renuse S, Pranaw K, Nain L. Proteomic analysis of Streptomyces sp. ssr-198 grown on
paddy straw. J Bas Microbiol. 2015; 55 (6):790-797. (Impact Factor: 1.82)
9. Singh S, Pranaw K, Singh B, Tiwari R, Nain L. Production, optimization and evaluation of multicomponent
holocellulase produced by Streptomyces sp. ssr-198. J Taiwan Inst Chem Eng. 2014;45:2379-86.
(Impact Factor: 3.00)
10. Kumar M, Nath Yadav A, Tiwari R, Prasanna R, Saxena AK. Evaluating the diversity of culturable
thermotolerant bacteria from four hot springs of India. J Biodivers Biopros Dev. 2014;1(127):2.
11. Pandey S, Kushwah J, Tiwari R, Kumar R, Somvanshi VS, Nain L, Saxena AK. Cloning and expression of β-
1, 4-endoglucanase gene from Bacillus subtilis isolated from soil long term irrigated with effluents of paper
and pulp mill. Microbiol Res. 2014;169(9-10):693-8. (Impact factor: 2.56)
12. Pandey S, Tiwari R, Singh S, Nain L, Saxena AK. Evaluation of β-1, 4-endoglucanases produced by bacilli
isolated from paper and pulp mill effluents irrigated soil. J Microbiol Biotechnol. 2014;24(8):1073–1080.
(Impact factor: 1.53)
2 | P a g e

3. 13. Pandiyan K, Tiwari R, Rana S, Arora A, Singh S, Saxena AK, Nain L. Comparative efficiency of different
pretreatment methods on enzymatic digestibility of Parthenium sp. World J Microbiol Biotechnol.
2014;30(1):55-64. (Impact factor: 1.78)
14. Tiwari S, Singh P, Tiwari R, Meena KK, Yandigeri M, Singh DP, Arora DK. Salt-tolerant rhizobacteria-
mediated induced tolerance in wheat (Triticum aestivum) and chemical diversity. Biol Fertil Soils.
2011;47:907-916. (Impact factor: 3.40)
15. Kumar M, Yadav AN, Tiwari R, Prasanna R, Saxena AK. Deciphering the diversity of culturable
thermotolerant bacteria from Manikaran hot springs. Ann Microbiol. 2014;64(2):741-751. (Impact factor:
0.99)
16. Saritha M, Tiwari R, Singh S, Nain PKS, Rana S, Adak A, Arora A, Nain L. Glycoside hydrolase production by
Aspergillus terreus CM20 using mixture design approach for enhanced enzymatic saccharification of alkali
pretreated paddy straw. Ind J Exp Biol. 2015. (Impact factor: 0.84)
17. Pandiyan K, Tiwari R, Singh S, Nain PK, Rana S, Arora A, Singh SB, Nain L. Optimization of enzymatic
saccharification of alkali pretreated Parthenium sp. using response surface methodology. Enzyme Res.
2014;2014:764898.
18. Saritha M, Tiwari R, Singh S, Rana S, Adak A, Sharma A, Arora A, Nain L. Bioprospecting for superior
biomass hydrolysing fungi from diverse habitats. Journal of Biodiversity, Bioprospecting and
Development. 2015; 2(2). 1000149.
19. Rana S, Adak A, Tiwari R, Sharma A, Saritha M, Singh S, Nain L. Pretreatment and Designing Energy Crops:
Technological Innovations and Prospects. Res J Microbiol. 2015;10 (12): 557-570
20. Rana S, Tiwari R, Arora A, Singh S, Kaushik R, Saxena AK, Datta SC, Nain L. Prospecting Parthenium sp.
pretreated with Trametes hirsuta, as a potential bioethanol feedstock. Biocatal Agric Biotechnol.
2013;2(2):152-158.
21. Tiwari R, Kaur J, Kumar A, Singh N. Bioinformatic analysis of Leishmania donovani long-chain fatty acid-
CoA ligase as a novel drug target. Mol Biol Int. 2011;2011:278051.
Book Chapters
 Rana S., Tiwari R., Pandiyan K., Arora A., Singh S., Saxena A.K. and Nain, L. (2013) Weedy
lignocellulosic biomass, a potential feedstock for bioethanol production: A future perspective. In: Recent
Advances in Bio-energy Research (Eds. Kumar, S. and Tayagi, S.K.), Sardar Swaran Singh National
Institute of Renewable Energy, Kapurthala, Punjab, (India). ISBN 978-81-927097-1-0. pp. 183-192.
 Amat D., Tiwari R, Arora A.,, Saxena A.K. and Lata (2013) Bioprospecting plant pathogen Xanthomonas
axonopodis pv. punicae for production of cellulases and xylanases for lignocellulosic biomass hydrolysis. In:
Recent Advances in Bio-energy Research (Eds. Kumar, S. and Tayagi, S.K.), Sardar Swaran Singh National
Institute of Renewable Energy, Kapurthala, Punjab, (India). ISBN 978-81-927097-1-0. pp. 144-159.
Popular Article
 Tiwari R, Rana S, Singh S, Arora A, Saxena AK and Nain L (2013) Myrothecium roridum LG7 delignifies
paddy straw. ICAR News 19(4):7.
SEMINARS & CONFERENCES
3 | P a g e

4. 1. Tiwari R, Singh S, Lata (2015) Phoma exigua: An arsenal of hydrolytic enzyme system for the
saccharification of lignocellulosic biomass. National Seminar on Recent Trends in Science & Technology to
be held at ISC Bhopal Chapter, 3031 March 2015. (Oral Presentation)
2. Tiwari R, Singh S, Lata (2015) Phoma exigua: An arsenal of hydrolytic enzyme system for the
saccharification of lignocellulosic biomass. In: National Seminar on Recent Trends in Science &
Technology held at Indian Science Congress Bhopal Chapter, 30-31 March 2015. (Oral presentation) pp
40-41.
3. Singh S, Pranaw, K., Singh, B., Tiwari R and Nain, L. (2014) Production, statistical optimization and
characterization of multicomponent holocellulase produced by Streptomyces sp. ssr-198 isolated from
desert soil. Paper presented in 84th
Annual Session of the National Academy of Sciences India
(NASI) held at JNV University, Jodhpur, Rajasthan (India) from 5-6th
Dec. 2014. (Oral presentation)
4. Rana S, Tiwari R, Adak A, Sharma A, Saritha M., Singh S, Lata. (2015) Bio Delignification Ability of
Bird’s Nest Fungus Cyathus Sterecoreus for Parthenium Sp. Biomass. Paper presented in 56th
Annual
conference of Association of Microbiologists of India (AMI-2015) from December 7-10, 2015 held at JNU,
New Delhi. PP. BPBE12 (Poster).
5. Priya S, Tiwari R, Rana S, Singh S, Saritha M, Arora A, Lata. (2015) Saccharification of Bio-pretreated
Paddy Straw with Indigenous Enzymes and Fermentation with Saccharomyces Cerevisiae LN1 under
Optimized Conditions. Paper presented in 56th
Annual conference of Association of Microbiologists of India
(AMI-2015) from December 7-10, 2015 held at JNU, New Delhi. PP. BPBE78 (Poster).
6. Adak A, Tiwari R, Sharma S, Singh S, Lata. (2015) Assessment of the Pretreatment Conditions for
Bioconversion of Parthenium Sp., A Weedy Lignocellulosic into Fermentable Sugar. Paper presented in
56th
Annual conference of Association of Microbiologists of India (AMI-2015) from December 7-10, 2015
held at JNU, New Delhi. PP. BPBE107 (Poster).
7. Tiwari R, Singh S, Shukla P, Lata. (2015) Bioprospecting of Cold Active β-glucosidase from
Pseudomonas Lutea BG8 Isolated from Himalayan Cold Desert. Paper presented in 56th
Annual conference
of Association of Microbiologists of India (AMI-2015) from December 7-10, 2015 held at JNU, New Delhi.
PP. EMP90 (Poster).
8. Saritha M, Tiwari R, Singh S, Arora A, Lata. (2015) Purification and Kinetic Characterization of
Endoglucanase Isoforms Produced By an Aspergillus terreus Isolate. Paper presented in 56th
Annual
conference of Association of Microbiologists of India (AMI-2015) from December 7-10, 2015 held at JNU,
New Delhi. PP. IMP27 (Poster).
9. Sharma A, Nain L, Tiwari R, Singh S, Lata. (2015) Optimization of Saccharification Process by Using
Novel Cold Tolerant Holocellulase Cocktail For Alkali Pretreated Corn Cob. Paper presented in 56th
Annual
conference of Association of Microbiologists of India (AMI-2015) from December 7-10, 2015 held at JNU,
New Delhi. PP. IMP150 (Poster).
10. Tiwari R, Singh S, Adak A, Rana S, Sharma A, Saritha M, Lata (2015) Microbial interventions for
increasing the efficiency of second generation bio-fuel from lignocellulosic biomass. National seminar on
recent advances in agricultural, biochemical and environmental biotechnology held in Department of
Biotechnology, Anand Engineering Collage, Agra, India from May 1-2, 2015, p. 16 (Invited lecture)
4 | P a g e

5. 11. Adak A, Tiwari R, Singh S, Sharma S, Lata (2015) Valorization of Parthenium sp. For laccase production
by a novel white rot fungi Pseudolagarobasidium acaciicola LA1 in solid state fermentation. National
seminar on recent advances in agricultural, biochemical and environmental biotechnology held in
Department of Biotechnology, Anand Engineering Collage, Agra, India from May 1-2, 2015, p. 48 (Poster)
12. Saritha M, Tiwari R, Singh S, Adak A, Arora A, Lata (2015) comparative profiling of solid state and
submerged production of glycoside hydrolases by Aspergillus terreus for enzymatic degradation of
lignocellulosic biomass. National seminar on recent advances in agricultural, biochemical and
environmental biotechnology held in Department of Biotechnology, Anand Engineering Collage, Agra,
India from May 1-2, 2015, p. 49. (Poster)
13. Anuraag B, Tiwari R, Sharma A, Singh S, Prasanna R, Lata (2015) Elucidating the mode of interactions
of zinc oxide nanoparticles with agriculturally beneficial bacteria. National seminar on recent advances in
agricultural, biochemical and environmental biotechnology held in Department of Biotechnology, Anand
Engineering Collage, Agra, India from May 1-2, 2015, p. 43. (Poster)
14. Adak A, Tiwari R, Rana S, Sharma A, Singh S, Arora A and Nain L. (2014) Delignification of paddy straw
and Parthenium sp. with a novel agaricomycete Pseudolagarobasidium acaciicola LA1 for improved
saccharification and simultaneous enzyme production. 55th Annual Conference of Association of
Microbiologists of India-EMMT-2014 held at Tamil Nadu Agricultural University, Coimbatore, India from
November 12-14, 2015 (Abstract) pp. IM95.
15. Saritha M, Tiwari R, Singh S, Nain PKS, Rana S, Adak A, Arora A and Nain L. (2014) Mixture design
approach to enhance glycoside hydrolases production by Aspergillus terreus CM20 for biorefinery
applications. 55th Annual Conference of Association of Microbiologists of India-EMMT-2014 held at Tamil
Nadu Agricultural University, Coimbatore, India from November 12-14, 2015 (Abstract) pp. IM126.
16. Tiwari R, Adak A, Shukla P, Lata. (2013) prospecting psychrotolerant bacteria from Kargil for cold
adaptive cellulases. 54th
Annual conference of Association of Microbiologists of India. International
symposium on frontier discoveries and innovations in microbiology and its interdisciplinary relevance held
at Maharshi Dayanand University, Rohtak, Haryana, India from November 17-20, 2013 (Abstract) pp.
520.
17. Adak A, Tiwari R, Rana S, Singh S, Arora A, Lata (2013) Secretome analysis of phytopathogenic fungus
Phoma exigua ITCC 2049 for saccharification of lignocellulosic biomass. 54th
Annual conference of
Association of Microbiologists of India. International symposium on frontier discoveries and innovations in
microbiology and its interdisciplinary relevance held at Maharshi Dayanand University, Rohtak, Haryana,
India from November 17-20, 2013 (Abstract) pp. 523.
18. Tiwari R, Rana S, Singh S, Arora A, Kaushik R, Saxena AK, Lata (2012) Prospecting a novel
micromycete M. roridum LG7 as a pretreatment option for biological delignification of paddy straw and
carrot grass. 53rd
annual International conference of Association of Microbiologists of India. International
conference on Microbial World: Recent Innovations and Future Trends held at KIIT University,
Bhubaneshwar, Odisha, India from November 22-25, 2012 (Abstract) pp. 98.
19. Tiwari R, Rana S, Arora A, Singh S, Saxena AK, Lata (2011) Evaluation of phytopathogenic fungus
Phoma exigua as an efficient strain for saccharification of
agricultural biomass. 52nd
annual conference of Association of Microbiologists of India, International
5 | P a g e

6. conference on microbial biotechnology and sustainable development held at Panjab University,
Chandigarh, India from November 3-6, 2011. (Abstract) pp. 186.
20. Rana S, Tiwari R, Arora A, Singh S, Saxena AK, Lata (2011) Comparative efficiency of white rot and
coprophilus fungi for hydrolysis of lignocellulosics. 52nd
annual conference of Association of Microbiologists
of India, International conference on microbial biotechnology and sustainable development held at Panjab
University, Chandigarh, India from November 3-6, 2011. (Abstract) pp. 67.
21. Tiwari S, Tiwari R, Saxena AK (2009) Field evaluation of bacterial consortia to alleviate salt stress for
growth and yield of wheat. 50th
Annual conference Association of Microbiologist of India on third golden
era of microbiology held at National Chemical Laboratory, Pune from December 15-18, 2009 (poster
abstract) pp. 69-70.
22. Tiwari R, Tiwari S, Singh P, Saxena AK, Arora DK (2008) Biochemical and molecular characterization of
salt-tolerant bacteria from Sambhar Lake, Rajasthan. 49th
annual conference of Association of
Microbiologist in India, International symposium on microbial Biotechnology: Diversity, Genomics and
Metagenomics held at Department of zoology, University of Delhi, New Delhi, India. December 18-20,
2008 (Abstract) pp. MD112.
23. Tiwari R, Rana S, Singh S, Arora A, Kaushik R, Saxena AK and Lata. Prospecting a novel Micromycetes
M. roridum as a pretreatment option for enhanced saccharification of paddy straw and carrot grass. Paper
presented in 2nd
National conference on Recent Advances in Bio-energy Research held at SSS-National
Institute of Renewable Energy (An Autonomous Institute of Ministry of New and Renewable Energy),
Kapurthala, Punjab, India from December 7-8, 2012.
24. Rana S, Tiwari R, Arora A, Singh S, Saxena AK and Lata. Perrenial weed Parthenium sp. preteated with
selected white rot fungus Trametes hirsuta, as a potential feedstock for bioethanol production. 2nd
National conference on Recent Advances in Bio-energy Research held at SSS-National Institute of
Renewable Energy (An Autonomous Institute of Ministry of New and Renewable Energy), Kapurthala,
Punjab, India from December 7-8, 2012.
25. Rana S, Tiwari R, Kaushik R, Singh S, Arora A, Saxena AK and Lata. Evaluation of selected white rot
fungi assisted biological pretreatment of carrot grass undergoing enzymatic hydrolysis by cellulase.
National seminar on Biotechnological intervention for the benefits of mankind held at Anand Engineering
College, Agra, India from February 2-4, 2012.
26. Tiwari R, Rana S, Singh S, Arora A, Saxena AK and Lata. Screening of plant pathogenic fungi for their
potential to produce hydrolytic enzymes facilitating the enhanced saccharification of lignocellulosic
biomass. National seminar on Biotechnological intervention for the benefits of mankind held at Anand
Engineering College, Agra, India from February 2-4, 2012.
27. Rana S, Tiwari R, Kaushik R, Singh S, Arora A, Saxena AK and Lata. Evaluation of selected white rot
fungi assisted biological pretreatment of carrot grass undergoing enzymatic hydrolysis by cellulose.
National seminar on Biotechnological intervention for the benefits of mankind held at Anand Engineering
College, Agra, India from February 2-4, 2012.
28. Tiwari S, Singh P, Tiwari R, Meena KK, Yandigeri M, Singh DP and Arora DK. Chemical diversity and
induced systemic tolerance in wheat (Triticum aestivum) rhizosphere with promotion of plant growth
enhanced by salt tolerant rhizobacteria. National Symposium on Harnessing Biodiversity for th Biological
Control of Crop Pests, Bangalore. May 25, 2011.
6 | P a g e

7. 29. Tiwari R, Rana S, Singh S, Arora A, Saxena AK and Lata. Screening of plant pathogenic fungi for their
potential to produce hydrolytic enzymes facilitating the enhanced saccharification of lignocellulosic
biomass. National seminar on Biotechnological intervention for the benefits of mankind held at Anand
Engineering College, Agra, India from February 2-4, 2012.
30. Tiwari R, Tiwari S, Singh P, Saxena AK Field evaluation of bacterial consortia to alleviate salt stress for
growth and yield of wheat. Poster presented in First Asian PGPR congress for sustainable agriculture.
Acharya NG Ranga Agricultural University Rajendranagar, Hyderabad, India. June 21‐24, 2009.
31. Das S, Singh K, Tiwari R, Singh N. Long chain fatty acyl CoA synthetase: A novel drug target for
chemotherapy against L. donovani. 4th
world conference on Leishmaniasis. Central Drug Research
Institute, Lucknow, India (Indian Chapter of WorldLeish) at Lucknow from February 3-7, 2009.
CONFERENCE proceedings
1. Singh S, Renuse S, Tiwari R and Nain L. (2015) Proteomic analysis of secretome of Streptomyces sp.
ssr-198 grown on paddy straw. In : Proceedings of 2nd
International Conference on Bioenergy
Environment and Sustainable Technologies (BEST 2015) held at Arunai Engineering College,
Tiruvannamalai, Tamilnadu (India) from January 28-31, 2015 (oral) pp109-110.
2. Tiwari R, Rana S, Singh S, Arora A, Kaushik R, Saxena AK and Lata (2012) Biological pretreatment:
Imperative step towards efficient production of second generation bioethanol. In : Proceedings of the
National seminar on Biotechnological Interventions for the benefits of mankind held at Department of
Biotechnology, Anand Engineering collage, Agra from February 2-4, 2012.
Sequences submitted to NCBI Gen Bank:
S. No. Sequence description Number of sequences
submitted to NCBI
1 Leishmania donovani Ld 2001, whole genome shotgun sequencing
project (ALJU01000000)
1
2. 16S ribosomal RNA gene, partial sequence 183
3. Uncultured bacterium clone 16S ribosomal RNA gene from
metagenome, partial sequence
153
4. Fungal and yeast 18S ribosomal RNA gene, partial sequence;
internal transcribed spacer 1, 5.8S ribosomal RNA gene, and
internal transcribed spacer 2, complete sequence; and 28S
ribosomal RNA gene, partial sequence
53
5. Endo-beta-1,4-glucanase gene from isolated strains and
metagenome, complete and partial CDS
30
6. β-glucosidase gene from metagenome, partial CDS 60
Total 480
WORKSHOP & TRAINING
7 | P a g e

8. • Workshop on “Renovating Bacterial Taxonomy with Bioinformatics” at Department of Zoology,
University of Delhi from May 14-15, 2015.
• National Training program on “Molecular and Serological Detection of Plant Viruses” at National
Bureau of Agriculturally Important Microorganisms (ICAR) from Feb-24 to Mar-1, 2008.
• Certificate course on Bio-informatics From Maulana Azad National Institute of Technology, Bhopal
from May-2 to Jun-1, 2005.
EXPOSURE TO COMPUTER
 Waters® Empower™ software for data integration and analysis of HPLC data.
 Minitab 16.2.1 and Design Expert 8.0.7.1 software for designing and analysis of the experiments.
 MASCOT search engine (Matrix Science,Boston, MA, USA) for mass fingerprinting of MS/MS spectra.
 MeV v4.9 (MultiExperiment Viewer) software as a microarray data analysis tool for clustering,
visualization classification, statistical and biological theme discovery.
 MEGA 5.0 (Molecular Evolutionary Genetics Analysis) was used to calculate evolutionary distances and
phylogenetic dendrogram construction by multiple sequence alignments using ClustalW version 1.8.
 NTSYSpc version 2.0 for cluster analysis.
 Windows (XP/Vista,7.0)
 Internet
 MS Office
TECHNICAL SKILLS & ABILITIES
Proteomics and enzyme technology
 Qualitative and quantitative estimation of different glycosyl hydrolases and other enzymes.
 Statistical design of experiments (Taguchi design, Response surface methodology and experimental
mixture design) for improving the microbial production of enzymes by optimizing different biochemical
and physical parameters.
 Enzyme studies related to kinetics, characterization and stability.
 One dimensional and two dimensional polyacrylamide gel electrophoresis for evaluation and
identification of differentially expressed proteins and other proteomic applications. Zymography
technique was performed for detection of cellulases and xylanases enzymes.
 Secretome profiling of Phoma exigua ITCC2049 and Streptomyces sp. ssr-198 by liquid chromatography
coupled tandem mass spectrometry (LC–MS/MS) based proteomics approach.
 Partial protein purification by ammonium sulphate and organic acid precipitation.
 Purification of recombinant protein (N- terminal His6 tag) by affinity chromatography using a Ni2+
-IDA
Hi-trap chelating sepharose coloumn by using FPLC (ÄKTAprime plus system prim view 5.0, Amersham
Biosciences).
Microbial Biotechnology
8 | P a g e

9.  Isolation and molecular identification of microbes (Bacteria, Fungi and Yeast) from different
environmental niches. Total 249 microbes were identified through ribosomal gene sequencing and
submitted to Genbank.
 Unculturable microbial community analysis by constructing 16S rRNA gene sequencing based plasmid
library construction. Total 79 partial 16S sequences have been submitted to Genbank.
 Mining of biocatalysts (Endo-beta-1,4-glucanase and beta-glucosidase) from metagenome by
Fosmid/plasmid library construction and sequence based primer designing.
 Cloning, expression and purification of genes from Bacillus subtilis and Leishmania donovani by
recombinant DNA technology in bacterial expression system.
 DNA fingerprinting by using Random Amplified Polymorphic DNA (RAPD) and Restriction Fragment
Length Polymorphism (RFLP) analysis.
Analytical Biochemistry
 Carbon utilization pattern of microbes were performed by Phynotypic microarray (Biolog, Inc., Hayward,
CA).
 Detection of carbohydrates, alcohols phenolics, and quercetin by using High performance liquid
chromatography (HPLC) system.
 Analysis of alcohols through Gas chromatography
 Structural and biochemical modification studies by Scanning Electron Microscope (SEM), scanning
electron microscopy coupled with energy dispersive X-ray analysis (SEM-EDXA), Fourier transformed
infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
Process Development
 Development of cellulase enzyme cocktail from pscychrotolerant Aspergillus niger SH3, Phytopathogenic
fungus Phoma exigua ITCC 2049, Pseudomonas lutea BG8 and Streptomyces sp. ssr-198 for efficient
saccharification of pretreated lignocellulosic biomass.
 Biological delignification of paddy straw and Parthenium for second generation biofuel production by a
new lignolytic micromycete fungus Myrothecium roridum LG7 isolated from decayed food.
 Development of salt tolerant microbial consortia for enhanced plant growth promotion of wheat in the
saline stressed soil.
Referees
 Dr. Lata
9 | P a g e

10. Principal Scientist
Division of Microbiology
Indian Agriculture Research Institute, New Delhi-110012
E-mail: latarajat@yahoo.co.in
 Dr. A.K. Saxena
Director
National Bureau of Agriculturally Important Microorganisms (NBAIM)
Kushmaur, Post Box. No. 6
Mau Nath Bhanjan-275103
E-mail: saxena461@yahoo.com
 Dr. S. K. Khare
Professor of Biochemistry
Enzyme and Microbial Biochemistry Lab
Department of Chemistry
Indian Institute of Technology, New Delhi-110016
E mail: skhare@rocketmail.com
skkhare@chemistry.iitd.ac.in
I hereby declare that all the information intimated above is true to best of my knowledge
(Rameshwar Tiwari)
10 | P a g e