1. MicroLiterLANALYTICALSUPPLIES,INC.
888-232-7840 • Try the new www.MicroLiter.com • 770-932-6565
PO Box 808, Suwanee, GA 30024
Master Card / Visa & American Express Corporate Procurement Card accepted
InLessThan4Hours
ITSPCanDevelopAScaledDownMethodWith
OptimizedAnalyticalParameters
Step 1. Blend Solvents - The first macro set blends two solvents
beginning with 100% in position one decreasing to 0% in position 11.
Likewise Solvent two starts with 0% in position 1 and ends with 100%
in position 11.
MicroLiter has developed a 96 well microplate
and a set of macros that automates method
development based on application notes
provided by most sorbent manufacturers. It is
also an excellent tool for scaling down methods
you belive can increase the productivity of your
lab while at the same time reducing the costs
of solvents, raw sample requirements and
hazardous waste disposal. Here’s how:
Solvent
Solvent
Raw
Solvent
Sample Load
Solvent
Raw
ITSP
Wash Solvent Blend Elution Solvent Blend
As illustrated the sample begins to elute at approximately 37% MeOH.
At approximately 70% Methanol the sample elution is complete and
both parameters are set. One other note: One for the chromatograms
was produced using an ITSP device twice proving in situations where
chain-of custody are not factors, the device can be reused.
Step 2. Solvent
Strength - This
macro simply loads
the device with
sample at incre-
ments of 10µL until
break through
occurs. In this case the blue food coloring begins to elute between 30
and 40µL.
Steps 3 and 4 Sample Breakthrough and Sample Load Capacity -
These steps are performs to either individually or simple step 4 by
itself. If the sample is relatively clean direct injection without a wash
can offer specific data for Sample Break Through. If the sample is
dirty it could damage the analytica column so the device may require
washing before and step offer offers this option. These macros
illustrate the 10mg C8 sorbent had no issues loading the sample
within the requirements of the assay.
Join us for a One Day Seminar on Tuesday at Pittcon for Detailed Instruction On Set-up and Operation of ITSP.
The Morning session details conversion of your PAL to ITSP including
a detailed explanation of the ITSP device, installation of the ITSP
Hardware Kit and specific macros that assist in robust performance .
The Afternoon session details programming, method development,
conversion to a fixed macro and integration into analytical instrument
operating systems. Space is limited so call today to reserve your space.